Immunosuppression during severe acute pancreatitis is associated with a dramatic shift in intestinal microbiota composition and infected necrosis

Pancreatology ◽  
2020 ◽  
Vol 20 ◽  
pp. S24
Author(s):  
M. Sendler ◽  
A. Wilden ◽  
J. Glaubitz ◽  
F. Frost ◽  
F. Weiss ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Severe Acute Pancreatitis ◽  
Intestinal Microbiota ◽  
Microbiota Composition ◽  
Infected Necrosis ◽  
Dramatic Shift

scholarly journals Saikosaponin A-Induced Gut Microbiota Changes Attenuate Severe Acute Pancreatitis through the Activation of Keap1/Nrf2-ARE Antioxidant Signaling

2020 ◽  
Vol 2020 ◽  
pp. 1-19
Author(s):  
Jing Li ◽  
Jinfeng Han ◽  
Juan Lv ◽  
Shiji Wang ◽  
Lai Qu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Pancreatitis ◽  
Gut Microbiota ◽  
Severe Acute Pancreatitis ◽  
Rat Model ◽  
Inflammatory Responses ◽  
Standard Diet ◽  
Microbiota Composition ◽  
Serum Lipase ◽  
Saikosaponin A

Objective. Severe acute pancreatitis (SAP) is a serious and life-threatening disease associated with multiple organ failure and a high mortality rate and is accompanied by distinct oxidative stress and inflammatory responses. Saikosaponin A has strong antioxidant properties and can affect the composition of gut microbiota. We sought to determine the effects of Saikosaponin A interventions on SAP by investigating the changes of gut microbiota and related antioxidant signaling. Methods. A SAP model was established in Sprague-Dawley (SD) rats through the injection of sodium taurocholate into the biliopancreatic duct and confirmed by elevated levels of serum lipase and amylase. The model was fed a standard diet either with saline solution or with Saikosaponin A. Fecal microbiota transplantation (FMT) from Saikosaponin A-induced rats into the rat model was performed to test the effects of gut microbiota. The composition of gut microbiota was analyzed by using 16S rRNA gene sequencing. We measured apoptotic status, inflammatory biomarkers, and Keap1-Nrf2-ARE ((Kelch-like ECH-associated protein 1) nuclear factor erythroid 2-related factor 2-antioxidant response element) antioxidant signaling. Results. Saikosaponin A intervention attenuated SAP lesions and reduced the levels of serum amylase and lipase, oxidative stress, and inflammatory responses by reducing pathological scores and affecting the serum level of oxidative and inflammatory factors. Meanwhile, the expression of Keap1-Nrf2-ARE was increased. Saikosaponin A intervention improved microbiota composition by increasing the relative abundance of Lactobacillus and Prevotella species. FMT resulted in similar results as those caused by the Saikosaponin A intervention, suggesting Saikosaponin A may exert its function via the improvement of gut microbiota composition. Conclusions. Saikosaponin A-induced gut microbiota changes attenuate SAP progression in the rat model and may be a potential natural drug for adjuvant treatment of SAP. Further work is needed to clear up the points.

Conservative management of infected necrosis complicating severe acute pancreatitis

2003 ◽  
Vol 98 (1) ◽  
pp. 98-103 ◽  
Author(s):  
Douglas G. Adler ◽  
Suresh T. Chari ◽  
Tamela J. Dahl ◽  
Michael B. Farnell ◽  
Randall K. Pearson
Keyword(s):  
Acute Pancreatitis ◽  
Severe Acute Pancreatitis ◽  
Conservative Management ◽  
Infected Necrosis

Soluble triggering receptor expressed on myeloid cells in severe acute pancreatitis: a biological marker of infected necrosis

2011 ◽  
Vol 38 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Zheng Lu ◽  
Yan Liu ◽  
Yuan-hang Dong ◽  
Xian-bao Zhan ◽  
Yi-qi Du ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Severe Acute Pancreatitis ◽  
Myeloid Cells ◽  
Biological Marker ◽  
Infected Necrosis
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