Gestational diabetes mellitus in in-vitro fertilization pregnancies – Clinical and placental histological characteristics

Gestational diabetes mellitus (GDM) may be related to intestinal mucosal damage and inflammation-induced dysbiosis of secretory IgA (SIgA) coated microbiota. SIgA coated L. reuteri can reduce the level of inflammation of GDM in vitro.

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miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus

Mediators of Inflammation ◽

10.1155/2019/4851214 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9

Author(s):

Pingping Wang ◽

Zengfang Wang ◽

Guojie Liu ◽

Chengwen Jin ◽

Quan Zhang ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Macrophage Polarization ◽

Vital Role ◽

Luciferase Reporter ◽

M2 Macrophage ◽

And Migration

MicroRNA (miRNA) has been widely suggested to play a vital role of in the pathogenesis of gestational diabetes mellitus (GDM). We have previously demonstrated that miR-657 can regulate macrophage inflammatory response in GDM. However, the role of miR-657 on M1/M2 macrophage polarization in GDM pathogenesis is not clear yet. This study is aimed at elucidating this issue and identifying novel potential GDM therapeutic targets based on miRNA network. miR-657 is found to be upregulated in placental macrophages demonstrated by real-time PCR, which can enhance macrophage proliferation and migration in vitro. Luciferase reporter assay shows the evidence that FAM46C is a target of miR-657. In addition, miR-657 can promote macrophage polarization toward the M1 phenotype by downregulating FAM46C in macrophages. The present study strongly suggests miR-657 is involved in GDM pathogenesis by regulating macrophage proliferation, migration, and polarization via targeting FAM46C. miR-657/FAM46C may serve as promising targets for GDM diagnosis and treatment.

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Anti‐Diabetic, Anti‐Inflammatory, and Anti‐Oxidant Effects of Naringenin in an In Vitro Human Model and an In Vivo Murine Model of Gestational Diabetes Mellitus

Molecular Nutrition & Food Research ◽

10.1002/mnfr.201900224 ◽

2019 ◽

Vol 63 (19) ◽

pp. 1900224 ◽

Cited By ~ 13

Author(s):

Caitlyn Nguyen‐Ngo ◽

Jane C Willcox ◽

Martha Lappas

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Murine Model ◽

Human Model ◽

Anti Oxidant ◽

Anti Inflammatory

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Influence of glucose on the in vitro production of IL-6 from peripheral blood cells of patients with and without gestational diabetes mellitus

Placenta ◽

10.1016/j.placenta.2015.01.446 ◽

2015 ◽

Vol 36 (4) ◽

pp. 488

Author(s):

T.F. Lobo ◽

K.P.T. Pendeloski ◽

R. Mattar ◽

M.R. Torloni ◽

B.Z. Souza ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Peripheral Blood ◽

Blood Cells ◽

Peripheral Blood Cells ◽

In Vitro Production ◽

Vitro Production

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miR-132 serves as a diagnostic biomarker in gestational diabetes mellitus and its regulatory effect on trophoblast cell viability

Diagnostic Pathology ◽

10.1186/s13000-019-0899-9 ◽

2019 ◽

Vol 14 (1) ◽

Cited By ~ 2

Author(s):

Xuegui Zhou ◽

Cuiping Xiang ◽

Xiaoxia Zheng

Keyword(s):

Diabetes Mellitus ◽

Cell Proliferation ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cell Function ◽

Fasting Blood Glucose ◽

Trophoblast Cell ◽

Operating Characteristics ◽

Diagnostic Potential

Abstract Background Gestational diabetes mellitus (GDM) leads to poor pregnancy outcomes. Strategies that improve trophoblast cell function are important methods for GDM treatment. This study aimed to investigate the expression and diagnostic potential of microRNA-132 (miR-132) in GDM patients, and further analyzed the effects of miR-132 on HTR-8/SVneo cell proliferation. Methods Quantitative real-time PCR was applied to estimate the expression of miR-132. A receiver operating characteristics curve (ROC) analysis was performed to evaluate the diagnostic value of serum miR-132 in GDM patients. In vitro regulation of miR-132 in trophoblast cell HTR-8/SVneo was achieved by cell transfection, and the effects of miR-132 on cell proliferation were assessed using CCK-8 assay. Results Expression of miR-132 was decreased in serum and placenta tissues in GDM patients compared with the healthy women. A negative correlation was found between the serum miR-132 levels and fasting blood glucose of the GDM patients. A ROC curve shown the serum miR-132 had considerable diagnostic accuracy with an area under the curve (AUC) of 0.898. High glucose (HG) treatment induced an inhibition in HTR-8/SVneo cell proliferation and the expression of miR-132. The overexpression of miR-132 in HTR-8/SVneo cells could markedly rescued the HG - induced suppressed cell proliferation. Conclusion All the data of this study revealed the reduced expression of miR-132 in serum and placenta tissues of GDM, and serum miR-132 serves a candidate biomarker in the diagnosis of GDM. miR-132 may act a protective role against GDM via enhancing the trophoblast cell proliferation.

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Case Report on Primary Infertility with Hypothyroidism and Diabetes Mellitus

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i58b34202 ◽

2021 ◽

pp. 263-266

Author(s):

Thool Bali ◽

Lokhande Shalini ◽

A. Lalawmpuii ◽

Ambule Kalyani ◽

Shendre Vaishnavi ◽

...

Keyword(s):

Diabetes Mellitus ◽

In Vitro Fertilization ◽

Sperm Count ◽

Vitro Fertilization ◽

Primary Infertility ◽

History Of ◽

Irregular Menses ◽

One Year ◽

Loss Of Appetite

Introduction: Infertility is the inability, by natural means, of an animal to reproduce. Typically, adult species are not in their normal state of health. A woman who is unable to conceive well will define infertility as unable to bear a full-term pregnancy. Because of any ejaculating disease, and any declining sperm count, men are directly liable for 30-40% infertility. The WHO estimates the overall prevalence of primary infertility in India at 3.9% and 16.8%. Fertility estimates differ widely between India and 3.7% in Utter Pradesh and Maharashtra. Case Presentation: On 9/12/2020, a 38-year-old female came for In Vitro Fertilization with a known case of primary infertility in AVBR Hospital, Wardha. Her complaint was inability to conceive for 4 years, irregular menses, headache, sleep disturbance, loss of appetite. She was admitted for in-vitro fertilization therapy for the 2nd cycle. She had a history of hypothyroidism for 8 years for which she has been taking Thyrox 50mg OD tablet and has Diabetes Mellitus for one year since she is taking Metformin 500mg BD tablet. Instead, she has no concerns about asthma, tuberculosis, epilepsy, etc. On 9/12/2020, she underwent an embryo transfer. Diagnostic Evaluation: The diagnostic hysteroscopy was conducted at the private hospital in Amravati 2 years ago. She has been diagnosed with nullipara for 4 years as a primary infertility. She has already undergone 2 cycles of Intra Uterine Insemination (IUI) and 1 cycle of in vitro fertilization. Hysterosalpingography: Both fallopian tubes are normal & patent uterus is normal. Conclusion: There is multifactorial infertility. In both men and women, anatomy, physiology, the environment, hormones and genetics all play a role in causing infertility. Therefore, in the coming years, it is a very important problem and research in this zone is very essential.

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miR-410 enhanced hESC-derived pancreatic endoderm transplant to alleviate gestational diabetes mellitus

Journal of Molecular Endocrinology ◽

10.1530/jme-15-0100 ◽

2015 ◽

Vol 55 (3) ◽

pp. 219-229 ◽

Cited By ~ 12

Author(s):

Yang Mi ◽

Na Guo ◽

Tongqiang He ◽

Jing Ji ◽

Zhibin Li ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Embryonic Stem ◽

Late Pregnancy ◽

Β Cells ◽

Pregnant Females ◽

Pancreatic Progenitors ◽

First Time

Gestational diabetes mellitus (GDM) is a condition commonly encountered during mid to late pregnancy with pathologic manifestations including hyperglycemia, hyperinsulinemia, insulin resistance, and fetal mal-development. The deficit and dysfunction of insulin secreting β-cells are signature symptoms for GDM. Pancreatic progenitors derived from human embryonic stem cells (hESCs) were shown to be able to effectively treat diabetes in mice. In this study, we first identified that microRNA-410 (miR-410) directly targets lactate dehydrogenase A (LDHA), a gene selectively repressed in normal insulin secreting β-cells. hESCs that can be induced to express miR-410 hence keeping LDHA levels in check were then differentiatedin vitrointo pancreatic endoderm, followed by transplantation intodb/+mouse model of GDM. The transplant greatly improved glucose metabolism and reproductive outcome of the pregnant females suffering from GDM. Our findings describe for the first time the method of combining miRNA with hESCs, providing proof of concept by employing genetically modified stem cell therapy for treating GDM.

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