Transcriptomic analysis of MA-10 tumor Leydig cells treated with adipose derived hormones adiponectin and resistin

Testicular biopsies were obtained from six young male subjects (age range 24-33) who complained of infertility and who had clinical evidence of oligospermia. This was confirmed on histological examination which showed a broad spectrum from profound hypospermatogenesis to relatively normal appearing germinal epithelium. Thickening of the tubular walls was noted in half of the cases and slight peritubular fibrosis in one. The Leydig cells were reported as normal or unremarkable.Transmission electron microscopy showed that the thickening of the supporting tissue of the germinal epithelium was caused more by an increase in the thickness of the layers of the lamina propria than of the tubular wall itself. The changes in the basement membrane of the tubular wall consisted mostly of a greater degree of infolding into the tubule and some reduplication which gave rise to a multilayered appearance.

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The structure of the gills of the axolotl, Ambystoma mexicanum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128407 ◽

1987 ◽

Vol 45 ◽

pp. 824-825

Author(s):

Mohinder S. Jarial

Keyword(s):

Leydig Cells ◽

Secretory Granules ◽

Light And Electron Microscopy ◽

Cell Types ◽

Ambystoma Mexicanum ◽

Basal Cells ◽

Granular Cells ◽

Gill Filaments ◽

Mitotic Figures ◽

Apical Membranes

The axolotl is a strictly aquatic salamander in which the larval external gills are retained throughout life. The external gills of the adult axolotl have been studied by light and electron microscopy for ultrastructural evidence of ionic transport. The thin epidermis of the gill filaments and gill stems is composed of 3 cell types: granular cells, the basal cells and a sparce population of intervening Leydig cells. The gill epidermis is devoid of muscles, and no mitotic figures were observed in any of its cells.The granular cells cover the gill surface as a continuous layer (Fig. 1, G) and contain secretory granules of different forms, located apically (Figs.1, 2, SG). Some granules are found intimately associated with the apical membrane while others fuse with it and release their contents onto the external surface (Fig. 3). The apical membranes of the granular cells exhibit microvilli which are covered by a PAS+ fuzzy coat, termed “glycocalyx” (Fig. 2, MV).

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Smooth endoplasmic reticulum in perfusion and immersion-fixed Leydig cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100157954 ◽

1990 ◽

Vol 48 (3) ◽

pp. 82-83

Author(s):

R.T.F. Bernard ◽

R.H.M. Cross

Keyword(s):

Endoplasmic Reticulum ◽

Electron Microscope ◽

Steroid Hormones ◽

Transmission Electron Microscope ◽

Leydig Cells ◽

Smooth Endoplasmic Reticulum ◽

Random Mixture ◽

Transmission Electron

Smooth endoplasmic reticulum (SER) is involved in the biosynthesis of steroid hormones, and changes in the organisation and abundance of this organelle are regularly used as indicators of changes in the level of steroidogenesis. SER is typically arranged as a meshwork of anastomosing tubules which, with the transmission electron microscope, appear as a random mixture of cross, oblique and longitudinal sections. Less commonly the SER appears as swollen vesicles and it is generally suggested that this is an artefact caused during immersion fixation or during immersion of poorly-perfused tissue.During a previous study of the Leydig cells of a seasonally reproducing bat, in which tissue was fixed by immersion, we noted that tubular SER and vesicular SER often occured in adjacent cells and sometimes in the same cell, and that the abundance of the two types of SER changed seasonally. We came to doubt the widelyheld dogma that vesicular SER was an artefact of immersion fixation and set out to test the hypothesis that the method of fixation does not modify the ultrastructure of the SER.

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"STEROID-CELL" ANTIBODY IN ENDOCRINE DISEASES

Acta Endocrinologica ◽

10.1530/acta.0.0760729 ◽

1974 ◽

Vol 76 (4) ◽

pp. 729-740 ◽

Cited By ~ 15

Author(s):

Peter Kamp ◽

Per Platz ◽

Jørn Nerup

Keyword(s):

Leydig Cells ◽

Addison’S Disease ◽

Addison's Disease ◽

Hormone Production ◽

Cortical Cells ◽

Endocrine Diseases ◽

Cell Antibody ◽

Indirect Immunofluorescence Technique ◽

Males And Females ◽

Adrenal Cortical Cells

ABSTRACT By means of an indirect immunofluorescence technique, sera from 116 patients with Addison's disease, an equal number of age and sex matched controls and 97 patients with other endocrine diseases were examined for the occurrence of antibody to steroid-producing cells in ovary, testis and adrenal cortex. Fluorescent staining was observed in the theca cells of growing follicles, the theca lutein cells, testicular Leydig cells and adrenal cortical cells, i. e. cells which contain enzyme systems used in steroid hormone production. The "steroid-cell" antibody was present in 24 % of the patients with idiopathic Addison's disease, equally frequent in males and females, and in 17 % of the patients with tuberculous Addison's disease, but was rarely found in controls, including patients with other endocrine diseases. Female hypergonadotrophic hypogonadism made an exception, since the "steroid-cell" antibody was found in about half the cases with this condition.

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