α-tocopherol prevents oxidative stress-induced proliferative dysfunction in first-trimester human placental (HTR-8/SVneo) cells

2022 ◽  
Vol 22 (1) ◽  
pp. 100602
Author(s):  
Lígia Pinto-Ribeiro ◽  
Cláudia Silva ◽  
Nelson Andrade ◽  
Fátima Martel
2015 ◽  
Vol 27 (6) ◽  
pp. 816-821 ◽  
Author(s):  
Agnieszka Żelaźniewicz ◽  
Judyta Nowak ◽  
Bogusław Pawłowski

2012 ◽  
Vol 24 (1) ◽  
pp. 153 ◽  
Author(s):  
E. B. Jeung ◽  
H. Yang

Preeclampsia is a pregnancy-specific disease characterised by de novo development of concurrent hypertension, proteinuria and oxidative stress in the placenta. In the placenta, intervillous blood flow increases after 10 weeks of gestation and results in exposure of trophoblast cells to oxygen. Hypoxia occurs during the development of placenta in the first trimester and is implicated in trophoblast differentiation. Ca2+ is a universal intracellular second messenger involved in many processes such as signal transduction, hormone secretion and programmed cell death. Human placental primary cell cultures were established from first-trimester human placentas (at 7 to 12 weeks of gestation). In this study, calcium-related proteins (CRPs; TRPV6, PMCA1, NCKX3 and CaBP-28k) were investigated at normoxia (5% CO2 in 95% air) or hypoxia (2% O2/93% N2/5%CO2) for 12 h in human placental cell line (BeWo) and human placental primary cell (hPC). We confirmed mRNA expression by real-time PCR and protein expression by Western blot analysis. The data were 2 or 3 individual experiments with triplicate samples and analysed by one-way ANOVA using Tukey's multiple comparison test. In hypoxia, the level of TRPV6 mRNA and protein was not changed, however, calcium transporters' (NCKX3, CaBP-28k) mRNA and protein expressions were significantly increased in hypoxic BeWo cell compared with control (normoxia). In addition, expression of PMCA1 mRNA and protein was decreased in hypoxic BeWo cells. In hPC, CRPs (TRPV6, PMCA1, NCKX3 and CaBP-28k) mRNA and protein expressions were significantly induced by hypoxic stress compared with control. These results, taken together, indicate that alterations of calcium transporters in hypoxic stress may be involved in calcium transport in the placenta and protection of the placental trophoblasts from the oxidative stress during the pregnancy.


Author(s):  
S. Vijaya ◽  
M. Mahalakshmi ◽  
I. Inbapriyanka

Background: Preeclampsia is a multi system disorder with placenta as the organ of origin and maternal endothelium being the organ of target.  According to recent studies, the cell free haemoglobin induces oxidative stress mediated damage to the blood placenta barrier with consequently elevated levels of HbF in maternal blood. Alpha 1 microglobulin is an endogenous protein with antioxidant property, present in elevated levels in maternal blood in response to oxidative stress. This fact forms the basis for our study. The objective of the present study was to establish association between high levels of fetal hemoglobin and alpha 1 microglobulin in plasma of pregnant women between 10 to 16 weeks of gestational age and subsequent development of preeclampsia.Methods: This was a prospective cohort study undertaken in the Department of Obstetrics and Gynaecology, ISO -KGH, between December 2016 to November 2017. A total of 100 pregnant women were included in the study after getting informed written consent. Both primigravida and multigravida, belonging to age group of 20 to 35 years (singleton/ multiple) between 10 to 16 weeks GA and with BMI between 16 to 35 kg/m2 were included in the study. A woman with Diabetes mellitus, Hypertension, Renal disease, Epilepsy and Vascular disorders were excluded from the study.Results: The cut off value for alpha 1 microglobulin was 1.86ng/ml and the cut off value of fetal haemoglobin was 1.92ng/ml above which the pregnant women develop preeclampsia.Conclusions: Higher values of fetal hemoglobin and alpha 1 microglobulin in pregnant women between 10 to 16 weeks gestational age positively correlates with development of preeclampsia in those women.


2009 ◽  
Vol 116 (5) ◽  
pp. 637-642 ◽  
Author(s):  
N Potdar ◽  
R Singh ◽  
V Mistry ◽  
MD Evans ◽  
PB Farmer ◽  
...  

2013 ◽  
Vol 32 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Hassan Boskabadi ◽  
Mahdieh Moeini ◽  
Fatemeh Tara ◽  
Shima Tavallaie ◽  
Hamidreza Saber ◽  
...  

Summary Background: Oxidative stress is thought to be a major contributor to complications during pregnancy, for example preeclampsia. However, reports regarding prooxidant-antioxidant balance in uncomplicated pregnancy are inconsistent. In this study, we aimed to compare the levels of oxidative stress in non-pregnant women with apparently normal pregnant women during the first trimester and at delivery. Methods: An assay for the determination of prooxidant-antioxidant balance (PAB) was used in this study, in which the prooxidant burden and the antioxidant capacity were measured simultaneously in a single assay. The levels of oxidative stress were determined in 85 non-pregnant and 64 primigravid pregnant women. Results: Demographic data and biochemical indices did not differ significantly between the groups. Differences between PAB values were significant based on one-way ANOVA analysis (P<0.001). Using a post hoc test, we observed a statistically significant increase in PAB values during the first trimester and last trimester (P<0.001). Conclusions: Normal pregnancy is associated with a change in the measure of redox status, as assessed by the PAB assay.


2020 ◽  
Author(s):  
Meihe LI ◽  
Minchao KANG ◽  
Peng AN ◽  
Huimin DANG ◽  
Xin XU

Abstract BackgroundThe normal function of the placenta at each time stage of pregnancy is essential to a successful outcome. Placental dysfunction and increased oxidative stress and autophagy are the cause of a series of severe pregnancy complications. Resveratrol is a potent antioxidant that has shown beneficial effects in many diseases. We aim to investigate whether excessive autophagy is associated with oxidative stress in the trophoblast oxidative stress model. Resveratrol was taken to clarify its role in excessive autophagy of placental trophoblasts. MethodsWe established an in vitro model of oxidative stress by exposing the human first-trimester extravillous trophoblast cell line HTR-8/SVneo to H2O2. Levels of autophagy-related proteins (LC3, Beclin-1, p53 and mTOR) were detected by western blot.ResultsTreatment with resveratrol significantly ameliorated H2O2-induced cytotoxicity, morphological damage, oxidative stress and autophagy. Mechanistically, resveratrol restored the levels of autophagy-related proteins including LC3-II, Beclin-1 and p53, mTOR that were dysregulated by H2O2.ConclusionsResveratrol may protect human trophoblasts against H2O2-induced oxidative stress by reducing excessive autophagy, thus ensuring the normal biological functions of trophoblasts.


Author(s):  
Eugene D. Albrecht ◽  
Jeffery S. Babischkin ◽  
Graham W. Aberdeen ◽  
Marcia G. Burch ◽  
Gerald J. Pepe

Uterine spiral artery remodeling (UAR) is essential for placental perfusion and fetal development. A defect in UAR underpins placental ischemia disorders, e.g. preeclampsia, that result in maternal systemic vascular endothelial oxidative stress and dysfunction and hypertension. We have established a model of impaired UAR by prematurely elevating maternal serum estradiol levels during the first trimester of baboon pregnancy. However, it is unknown whether this experimental paradigm is associated with maternal vascular endothelial dysfunction. Therefore, in the present study baboons were administered estradiol on days 25-59 of gestation to suppress UAR and vascular function determined in maternal skeletal muscle obtained on day 165 (term = 184 days). Maternal peripheral serum sFlt-1 levels were 3-fold higher (P <0.05) and skeletal muscle arteriolar endothelial nitric oxide synthase protein expression and luminal area, and skeletal muscle capillary density, were 30-50% lower (P < 0.01) near term in UAR suppressed baboons. Maternal skeletal muscle catalase protein levels, reflecting oxidative stress, were 75% higher (P < 0.05) and mean arterial blood pressure 28% higher (P < 0.01) in UAR defective baboons. We propose that these changes in skeletal muscle, a systemic tissue, were induced by the elevation in sFlt-1, which suppresses vascular endothelial growth factor bioavailability. In summary, maternal skeletal muscle vascular function was disrupted in a baboon model of impaired UAR. These results highlight the translational impact of this primate model and relevance to adverse conditions of human pregnancy underpinned by improper uterine artery transformation.


2018 ◽  
Vol 26 (8) ◽  
pp. 1054-1061 ◽  
Author(s):  
Andrea Busnelli ◽  
Debora Lattuada ◽  
Stefania Ferrari ◽  
Marco Reschini ◽  
Barbara Colciaghi ◽  
...  

Inflammation and oxidative stress are intrinsically linked to early poor placentation, typical of pregnancies complicated by preeclampsia associated with intrauterine growth restriction (PE-IUGR). Low mitochondrial DNA copy number (mtDNAcn) in peripheral blood constitutes a good peripheral surrogate marker of inflammation and oxidative stress. On these basis, we explored a possible correlation between mtDNAcn in peripheral blood in the first trimester of pregnancy and the PE-IUGR onset. To shed light on this issue, we setup a nested case–control study from a prospective cohort of pregnant women undergoing first-trimester aneuploidies screening. Two groups of patients affected by PE classified according to the clinical phenotype were identified: (1) patients who developed PE-IUGR and (2) patients who developed PE associated with appropriate for gestational age intrauterine fetal growth (PE-AGAf). Controls were women with a physiologic pregnancy matched to cases on the basis of age (±6 months, ratio 2:1). Mitochondrial DNA copy number was quantified using real-time polymerase chain reaction and normalized to nuclear DNA. The median (interquartile range) mtDNAcn in peripheral blood in patients with PE-IUGR (n = 12) and in patients with PE-AGAf (n = 16) was 70 (44-97) and 108 (95-145), respectively ( P = .004). Both these values were significantly lower than that detected in the control group (161[133-183], P < .001). The area under the receiver–operator curve for PE-IUGR and PE-AGAf were 0.94 (95% confidence interval [CI]: 0.88-1.00, P < .001) and 0.81 (95%CI: 0.70-0.91, P < .001), respectively. In conclusion, MtDNAcn in peripheral blood resulted significantly lower both in patients affected by PE-IUGR and in those affected by PE-AGAf when compared to controls. The accuracy of this biomarker resulted particularly good in predicting PE-IUGR.


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