Evolution of volatile compounds composition during grape berry development at the germplasm level

Phytohormones strongly influence growth, development and nutritional quality of agricultural products by modulating molecular and biochemical changes. The purpose of the present study was to investigate the influence of root restriction (RR) treatment on the dynamic changes of main phytohormones during the berry development and ripening of “Summer Black” early ripening seedless grape (Vitis vinifera × V. labrusca), and to analyze the changes in the biosynthesis and signal transduction pathways of phytohormones by transcriptomics. Enzyme-linked immunosorbent assay (ELISA) and Ultra Performance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS) were used to quantify the phytohormone levels, and RNA-Seq was used to analyze the transcript abundance. The results showed that 23 transcripts involved in the phytohormone biosynthesis and 34 transcripts involved in the signal transduction pathways were significantly changed by RR treatment. RR also increased abscisic acid, brassinosteroid, ethylene, jasmonic acid and salicylic acid levels, while decreasing auxin, cytokinin, and gibberellin contents. The results of the present study suggest that RR treatment can accelerate the grape ripening process, and specific candidate genes were identified for further functional analysis.

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Interactions between auxin and quercetin during grape berry development

Scientia Horticulturae ◽

10.1016/j.scienta.2016.04.018 ◽

2016 ◽

Vol 205 ◽

pp. 45-51

Author(s):

Mei Luo ◽

Sibao Wan ◽

Xiangyu Sun ◽

Tingting Ma ◽

Weidong Huang ◽

...

Keyword(s):

Grape Berry ◽

Berry Development

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Transcriptomic Analysis of Root Restriction Effects on Phenolic Metabolites during Grape Berry Development and Ripening

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.0c02488 ◽

2020 ◽

Vol 68 (34) ◽

pp. 9090-9099

Author(s):

Feng Leng ◽

Jinping Cao ◽

Zhiwei Ge ◽

Yue Wang ◽

Chenning Zhao ◽

...

Keyword(s):

Grape Berry ◽

Transcriptomic Analysis ◽

Berry Development ◽

Phenolic Metabolites ◽

Root Restriction

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Salicylic acid modulated flavonol biosynthesis in three key phases during grape berry development

European Food Research and Technology ◽

10.1007/s00217-013-2008-8 ◽

2013 ◽

Vol 237 (3) ◽

pp. 441-448 ◽

Cited By ~ 3

Author(s):

Fang Fang ◽

Wei-Dong Huang

Keyword(s):

Salicylic Acid ◽

Grape Berry ◽

Berry Development

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System-Level and Granger Network Analysis of Integrated Proteomic and Metabolomic Dynamics Identifies Key Points of Grape Berry Development at the Interface of Primary and Secondary Metabolism

Frontiers in Plant Science ◽

10.3389/fpls.2017.01066 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 25

Author(s):

Lei Wang ◽

Xiaoliang Sun ◽

Jakob Weiszmann ◽

Wolfram Weckwerth

Keyword(s):

Network Analysis ◽

Secondary Metabolism ◽

Grape Berry ◽

System Level ◽

Berry Development ◽

Primary And Secondary Metabolism ◽

Key Points

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Grape berry development : A review

OENO One ◽

10.20870/oeno-one.2002.36.3.970 ◽

2002 ◽

Vol 36 (3) ◽

pp. 109 ◽

Cited By ~ 13

Author(s):

Paraskevi Diakou-Verdin ◽

Jean-Pierre Carde ◽

Jean-Pierre Gaudillère ◽

François Barrieu ◽

Nathalie Ollat ◽

...

Keyword(s):

Gene Expression ◽

Sugar Metabolism ◽

Growth Period ◽

Early Growth ◽

Grape Berry ◽

Special Focus ◽

Berry Development ◽

Growth Structure ◽

Berry Size ◽

Highly Sensitive

<p style="text-align: justify;">Grape berry development is reviewed with special focus on berry growth, structure, substances imported, organic acid and sugar metabolism. Berry growth is divided into two growth periods. Berry structure and ultra structure are adapted to sink function. Exocarp cells are characterized by intensive metabolic capacities, flesh cells by a storage role. Early growth is highly sensitive to internal and external parameters. Berry size is largely defined during the first growth period. After "véraison", the berry becomes a major storage sink. Many changes occur in berry metabolism and gene expression. Genomic researches are promising to elucidate the mechanisms of berry development.</p>

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A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation

Journal of Experimental Botany ◽

10.1093/jxb/eri265 ◽

2005 ◽

Vol 56 (420) ◽

pp. 2721-2731 ◽

Cited By ~ 143

Author(s):

Sandrine Mathieu ◽

Nancy Terrier ◽

Jérôme Procureur ◽

Frédéric Bigey ◽

Ziya Günata

Keyword(s):

Vitis Vinifera ◽

Functional Characterization ◽

Grape Berry ◽

Vitis Vinifera L ◽

Carotenoid Cleavage Dioxygenase ◽

Berry Development

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Iso-Seq allows genome-independent transcriptome profiling of grape berry development

10.1101/269530 ◽

2018 ◽

Cited By ~ 1

Author(s):

Andrea Minio ◽

Mélanie Massonnet ◽

Rosa Figueroa-Balderas ◽

Amanda M. Vondras ◽

Barbara Blanco-Ulate ◽

...

Keyword(s):

Single Molecule ◽

Transcriptional Profiling ◽

Transcriptome Profiling ◽

Grape Berry ◽

Cabernet Sauvignon ◽

Berry Development ◽

Grape Berries ◽

Gene Space ◽

A Genome ◽

Computationally Intensive

AbstractTranscriptomics has been widely applied to study grape berry development. With few exceptions, transcriptomic studies in grape are performed using the available genome sequence, PN40024, as reference. However, differences in gene content among grape accessions, which contribute to phenotypic differences among cultivars, suggest that a single reference genome does not represent the species’ entire gene space. Though whole genome assembly and annotation can reveal the relatively unique or “private” gene space of any particular cultivar, transcriptome reconstruction is a more rapid, less costly, and less computationally intensive strategy to accomplish the same goal. In this study, we used single molecule-real time sequencing (Iso-Seq) to sequence full-length cDNA and reconstruct the transcriptome of Cabernet Sauvignon berries during berry ripening. In addition, Illumina short reads from ripening berries were used to error-correct low-expression isoforms and to profile isoform expression. By comparing the annotated gene space of Cabernet Sauvignon to other grape cultivars, we demonstrate that the transcriptome reference built with Iso-Seq data represents most of the expressed genes in the grape berries and includes 1,501 cultivar-specific genes. Iso-Seq produced transcriptome profiles similar to those obtained after mapping on a complete genome reference. Together, these results justify the application of Iso-Seq to identify cultivar-specific genes and build a comprehensive reference for transcriptional profiling that circumvents the necessity of a genome reference with its associated costs and computational weight.

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Influence of Light on Grape Berry Growth and Composition Varies during Fruit Development

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.121.5.869 ◽

1996 ◽

Vol 121 (5) ◽

pp. 869-874 ◽

Cited By ~ 128

Author(s):

N.K. Dokoozlian ◽

W.M. Kliewer

Keyword(s):

Fruit Development ◽

Light Exposure ◽

Grape Berry ◽

Pinot Noir ◽

Stage Iii ◽

Cabernet Sauvignon ◽

Fruit Softening ◽

Berry Development ◽

Development Stages ◽

Final Sample

Potted `Cabernet Sauvignon' and `Pinot noir' grapevines (Vitis vinifera L.) grown in a sunlit phytotron were used to study the influence of cluster light exposure during various stages of fruit development on berry growth and composition. Clusters grown without light during berry development stages I and II, stage III, or stages I, II, and III, were compared to clusters exposed to light throughout fruit development (control). The temperature of light-exposed and nonexposed fruit was similar. The weights and diameters of berries grown without light during stages I and II, or stages I, II, and III, were similar and significantly lower than those of the control. Fruit softening in both cultivars, as well as the initiation of berry coloration, was delayed when berries were grown without light during stages I and II. Following fruit softening, berries grown without light during stages I, II, and III were lower in sugar than the control. On the final sample date, `Cabernet Sauvignon' berries grown without light during stages I, II, and III were higher in malate compared to the control. `Pinot noir' berries grown without light during stages I and II, or stages I, II, and III, were lower in malate before fruit softening, and higher in malate following fruit softening, than the control. Control berries had greater skin anthocyanins and phenolics compared to the remaining treatments. Berries grown without light during stages I and II, or stage III, were greater in anthocyanins and phenolics than fruit grown without light during stages I, II, and III. Light had no effect on fruit tartrate concentration or juice pH. Light had its greatest impact on fruit development during the initial stages of berry growth. Berry growth was reduced and ripening delayed when fruit were grown without light during stages I and II. Normal fruit development was not fully restored when these fruit were exposed to light during stage III.

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