Effects of flow intermittency and pharmaceutical exposure on the structure and metabolism of stream biofilms

2015 ◽  
Vol 503-504 ◽  
pp. 159-170 ◽  
Author(s):  
Natàlia Corcoll ◽  
Maria Casellas ◽  
Belinda Huerta ◽  
Helena Guasch ◽  
Vicenç Acuña ◽  
...  
Keyword(s):  
Stream Biofilms ◽  
Flow Intermittency ◽  
Structure And Metabolism

scholarly journals Addendum to Tolerance Patterns in Stream Biofilms Link Complex Chemical Pollution to Ecological Impacts

2020 ◽  
Vol 55 (1) ◽  
pp. 807-807 ◽  
Author(s):  
Ahmed Tlili ◽  
Natàlia Corcoll ◽  
Åsa Arrhenius ◽  
Thomas Backhaus ◽  
Juliane Hollender ◽  
...  
Keyword(s):  
Ecological Impacts ◽  
Chemical Pollution ◽  
Complex Chemical ◽  
Stream Biofilms

scholarly journals Molecular Characterization of Ciliate Diversity in Stream Biofilms

2008 ◽  
Vol 74 (6) ◽  
pp. 1740-1747 ◽  
Author(s):  
Andrew Dopheide ◽  
Gavin Lear ◽  
Rebecca Stott ◽  
Gillian Lewis
Keyword(s):  
18S Rrna ◽  
Pcr Primers ◽  
18S Rrna Gene ◽  
Sequence Diversity ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Specific Pcr ◽  
Stream Biofilms

ABSTRACT Free-living protozoa are thought to be of fundamental importance in aquatic ecosystems, but there is limited understanding of their diversity and ecological role, particularly in surface-associated communities such as biofilms. Existing eukaryote-specific PCR primers were used to survey 18S rRNA gene sequence diversity in stream biofilms but poorly revealed protozoan diversity, demonstrating a need for protozoan-targeted primers. Group-specific PCR primers targeting 18S rRNA genes of the protozoan phylum Ciliophora were therefore designed and tested using DNA extracted from cultured protozoan isolates. The two most reliable primer combinations were applied to stream biofilm DNA, followed by cloning and sequencing analysis. Of 44 clones derived from primer set 384F/1147R, 86% were of probable ciliate origin, as were 25% of 44 clones detected by primer set 121F/1147R. A further 29% of 121F/1147R-detected clones matched sequences from the closely related phylum Apicomplexa. The highly ciliate-specific primer set 384F/1147R was subsequently used in PCRs on biofilm DNA from four streams exhibiting different levels of human impact, revealing differences in ciliate sequence diversity in samples from each site. Of a total of 240 clones, 73% were of probable ciliate origin; 54 different putative ciliate sequences were detected from throughout seven taxonomic ciliate classes. Sequences from Oligohymenophorea were most commonly detected in all samples, followed by either Spirotrichea or Phyllopharyngea. Restriction fragment length polymorphism profile-based analysis of clones suggested a potentially higher level of diversity than did sequencing. Nevertheless, newly designed PCR primers 384F/1147R were considered to provide an effective molecular basis for characterization of ciliate diversity in stream biofilms.

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