Simultaneous hydrolysis of various protein-rich industrial wastes by a naturally evolved protease from tannery wastewater microbiota

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L-1 were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.

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Whole Conversion of Agro-Industrial Wastes Rich in Galactose-Based Carbohydrates into Lipid Using Oleaginous Yeast Aureobasidium Namibiae

10.21203/rs.3.rs-400653/v1 ◽

2021 ◽

Author(s):

Zhi-Peng Wang ◽

Jing-Run Ye ◽

Yan Ma ◽

Xin-Yue Zhang ◽

Hai-ying Wang ◽

...

Keyword(s):

Oleaginous Yeast ◽

Raw Materials ◽

Industrial Wastes ◽

Lactose Hydrolysis ◽

Efficient Utilization ◽

Oleaginous Yeasts ◽

Soy Molasses ◽

First Time ◽

Hydrolysis Of ◽

Direct Hydrolysis

Abstract BackgroundRaw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. ResultsIn this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. ConclusionsThis is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess

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Enzymatic hydrolysis of cellulose from agricultural and industrial wastes

Biological Wastes ◽

10.1016/0269-7483(88)90092-4 ◽

1988 ◽

Vol 25 (4) ◽

pp. 309-313 ◽

Cited By ~ 2

Author(s):

S.L. Gulati ◽

A.C. Gaur

Keyword(s):

Enzymatic Hydrolysis ◽

Industrial Wastes ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

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Microbial assisted industrially important multiple enzymes from fish processing waste: purification, characterization and application for the simultaneous hydrolysis of lipid and protein molecules

RSC Advances ◽

10.1039/c6ra11867d ◽

2016 ◽

Vol 6 (96) ◽

pp. 93602-93620 ◽

Cited By ~ 9

Author(s):

S. Hepziba Suganthi ◽

K. Ramani

Keyword(s):

Alkaline Lipase ◽

Fish Processing ◽

Processing Waste ◽

Protein Molecules ◽

Protein Rich ◽

Hydrolysis Of

Fish processing waste (FPW) was evaluated as the substrate for the concomitant production of industrially important alkaline lipase and protease byStreptomyces thermolineatusfor the hydrolysis of lipid and protein rich FPW.

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Whole conversion of agro-industrial wastes rich in galactose-based carbohydrates into lipid using oleaginous yeast Aureobasidium namibiae

Biotechnology for Biofuels ◽

10.1186/s13068-021-02031-8 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Zhi-Peng Wang ◽

Xin-Yue Zhang ◽

Yan Ma ◽

Jing-Run Ye ◽

Jing Jiang ◽

...

Keyword(s):

Oleaginous Yeast ◽

Raw Materials ◽

Lipid Production ◽

Industrial Wastes ◽

Lactose Hydrolysis ◽

Oleaginous Yeasts ◽

Soy Molasses ◽

First Time ◽

Hydrolysis Of ◽

Direct Hydrolysis

Abstract Background Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. Results In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. Changes of transcriptional levels indicated GalB and GalC were key α-galactosidases, and GalG was key β-galactosidase. In 10 L fermentor, lipid production from SM and WP achieved 6.45 g/L and 6.13 g/L, respectively. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. Conclusions This is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess. Graphic abstract

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Recombinant DNA technology as a tool for improving production of polyhydroxyalkanoates by the natural producers

Nova Biotechnologica et Chimica ◽

10.36547/nbc.v19i2.765 ◽

2020 ◽

Vol 19 (2) ◽

pp. 124-137

Author(s):

Daniela Chmelová ◽

Barbora Legerská ◽

Miroslav Ondrejovič

Keyword(s):

Culture Medium ◽

Recombinant Dna ◽

Low Cost ◽

Dry Weight ◽

Industrial Wastes ◽

Recombinant Dna Technology ◽

Protective Mechanisms ◽

Over Expression ◽

Pha Production ◽

Hydrolysis Of

Polyhydroxyalkanoates (PHAs) are a group of the biodegradable polyesters, and represent an alternative to conventionally used petroleum-based plastics resistant to biodegradation. The production is not cost-competitive compared to conventional plastics, although, there are several bacterial producers capable of PHA accumulating up to 80 % of their cells dry weight using low-cost substrates. PHA production can be improved by transferring specific enzymes or entire metabolic pathways from the most efficient producers to other natural producers. Therefore, the review is focused on genetic modification of bacterial producers, namely the genera Cupriavidus, Pseudomonas, Halomonas, Aeromonas and Bacillus, for efficient industrial production of PHAs. Recombinant PHA producer can use non-traditional substrates like agro-industrial wastes, namely whey, lignocellulose or glycerol. It is possible to influence the shape and size of the producer's cell by over-expression or knockout of selected genes or to affect its preference for a specific component of a culture medium by modulation of a producer's basal metabolism. The costs of PHA production still be reduced by simplifying the downstream process by enzymatic hydrolysis of selected parts of the cell or blocking the protective mechanisms of the cell against its autolysis caused by the ionic strength of the solution.

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Amylase-Producing Fungi and Bacteria Associated with Some Food Processing Wastes

Nigerian Journal of Biotechnology ◽

10.4314/njb.v38i1.9 ◽

2021 ◽

Vol 38 (1) ◽

pp. 74-82

Author(s):

T. Okunwaye ◽

P.O. Uadia ◽

B.O. Okogbenin ◽

E.A. Okogbenin ◽

D.C. Onyia ◽

...

Keyword(s):

Amylase Activity ◽

Bacterial Species ◽

Bacterial Count ◽

Industrial Wastes ◽

Bacterial Strains ◽

Glycosidic Bonds ◽

Processing Wastes ◽

Food Processing Wastes ◽

Hydrolysis Of ◽

Coconut Meat

Amylases are enzymes that catalyze the hydrolysis of glycosidic bonds present in starch to release simple sugars. They are one of the most important enzymes in numerous commercial processes. In this investigation, fungal and bacterial strains from the following agro-industrial wastes were isolated and screened for amylolytic ability: soil from oil palm plantation, shea seed, date fruit, coconut meat, cassava effluent, cassava peel, cassava tubers, yam and potato tubers, starch medium, parboiled water from noodles and rice. The results revealed the presence of Geotrichum, Aspergillus, Penicillium, Trichoderma, Rhizopus and Fusarium spp. Five major genera of bacterial species namely Corynebacterium, Pseudomonas, Lactobacillus, Micrococcus and Bacillus were isolated and screened for amylase activity. Cassava soil had the highest heterotrophic bacterial count of 5.7 x105cfu/g and coconut meat waste had the lowest heterotrophic bacterial count of 1.3 x105cfu/g. All isolated microorganisms had the amylolytic ability. The fungal isolates had higher amylase activity when compared with the bacterial isolates. This investigation reveals organisms with high amylase activity.

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