Rational design of bienzyme nanoparticles-based total cholesterol electrochemical sensors and the construction of cholesterol oxidase expression system

2021 ◽  
Vol 349 ◽  
pp. 130742
Author(s):  
Min Woo Kim ◽  
Yeong Hyeock Kim ◽  
Jyotiranjan Bal ◽  
Ruth Stephanie ◽  
Seung Hoon Baek ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Rational Design ◽  
Electrochemical Sensors ◽  
Cholesterol Oxidase ◽  
Expression System

scholarly journals Effect on lipid profile due to prolong Valproic acid intake

2021 ◽  
Vol 15 (7) ◽  
pp. 1497-1499
Author(s):  
Rao. S Aziz ◽  
Usman Saeed ◽  
Liaqat Ali ◽  
Muhammad Arshad ◽  
Roman Abbas ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Cholesterol Oxidase ◽  
Venous Blood ◽  
Case Group ◽  
Long Duration ◽  
And Control ◽  
Relationship Of

Background: Valproic acid (VA) serve as the antimigraine , anti-mental disturbances agent and antiepileptic medicine. After using va, metabolic rearrangements seen in patients that include alteration in lipoproteins levels; Aim: To discuss the effects of VA after using for long duration on total levels of cholesterol in adult. Methods: About Eighty candidates participated and they were divided into two groups namely, case group (40 candidates) and control groups (40 candidates). All the candidates were asked for collection of venous blood sample in order to determine total cholesterol serum level among them via aid of enzymatic cholesterol oxidase phenol 4-aminoantipyrine peroxidase. Results: By the aid of the logistic regression analysis, the relationship of the long-term VA treatment and the level of total cholesterol was obtained. With respect to our analysis, there is a co relation between total levels of cholestrol and long term usage of VA (P=0.003). Conclusions: In a net shell, by using VA for long duration, the total level of cholesterol in adults reduces. Keywords: Lipid profile, side effects, total cholesterol, valproic acid

Direct Fluorometric Determination of Total Cholesterol in Serum Using Derivatized Cholesterol Oxidase

2000 ◽  
Vol 54 (8) ◽  
pp. 1157-1162 ◽  
Author(s):  
Javier Galbán ◽  
José F. Sierra ◽  
José M. López Sebastian ◽  
Susana de Marcos ◽  
Juan R. Castillo
Keyword(s):  
Blood Serum ◽  
Total Cholesterol ◽  
Cholesterol Oxidase ◽  
Enzymatic Reaction ◽  
Direct Determination ◽  
Analytical Signal ◽  
Cholesterol Concentration ◽  
Serum Samples ◽  
Response Range

In this paper the use of cholesterol oxidase derivatized with a fluorescein derivative is proposed for the direct determination of total cholesterol in blood serum. The method is based on the changes in the fluorescence of the solution during the enzymatic reaction (λexe = 498 nm and λem 519 nm). A mathematical model which relates the analytical signal to the total cholesterol concentration was developed, and the model can also be used to obtain some of the thermodynamic constants of the process. The method has a linear response range up to 70 mg/L of cholesterol, a detection limit of 2.5 mg/L, and the precision was 1.0% (40 mg/L cholesterol, n = 10). The method was applied to total cholesterol determination in blood serum samples. The results were compared to those obtained by a commercial analyzer, and statistically similar results were obtained. The use of derivatized cholesterol oxidase makes it possible to simplify the methodology normally used in this type of determination (the indicator reaction is avoided and the number of reagents reduced), with the added advantage that the analytical signal is independent of the concentrations of O2 and cholesterol oxidase.

An enzymatic assay for determining free and total cholesterol in tissue.

1978 ◽  
Vol 24 (3) ◽  
pp. 433-435 ◽  
Author(s):  
J L De Hoff ◽  
L M Davidson ◽  
D Kritchevsky
Keyword(s):  
Total Cholesterol ◽  
Rat Liver ◽  
Ferric Chloride ◽  
Cholesterol Oxidase ◽  
Enzymatic Assay ◽  
Cholesterol Esterase ◽  
Enzymatic Method ◽  
Liver Lipids

Abstract We describe a method for determining free and total cholesterol in extracts of rat-liver lipids by use of a cholesterol esterase/cholesterol oxidase enzymatic kit (Boehringer Mannheim Corp). The lipids are solubilized (as micelles) directly into the aqueous reagent mixture. Results of the enzymatic method correlate well with those obtained by an acid/ferric chloride assay of digitonin precipitates of the extracts. The enzymatic assay of free and total cholesterol in tissue offers advantages of simplicity over previously used methods.

Automated enzymic measurement of total cholesterol in serum.

1978 ◽  
Vol 24 (1) ◽  
pp. 108-114 ◽  
Author(s):  
G S Rautela ◽  
R J Liedtke
Keyword(s):  
Hydrogen Peroxide ◽  
Total Cholesterol ◽  
Response Surface ◽  
Computer Analysis ◽  
Free Cholesterol ◽  
Cholesterol Oxidase ◽  
Cholesterol Esters ◽  
Direct Proportion ◽  
Analytical Test ◽  
Response Surface Designs

Abstract We describe a completely automated enzymic system for measuring total cholesterol in serum. All reagents are contained in an analytical test pack and the test is performed on Du Pont's Automatic Clinical Analyzer (aca), which mixes the sample (20 microliter) and reagents and performs the necessary absorbance measurements and calculations. In the procedure, cholesterol oxidase oxidizes free cholesterol. The oxidation step produces cholest-4-en-3-one and hydrochloride peroxidesterase hydrolyzes cholesterol esters and cholesterol in direct proportion to the amount of cholesterol present. N,N-Diethylaniline hydrochloride and 4-aminoantipyrine react with the hydrogen peroxide to produce a quinoneimine dye (lambda max = 553 nm). Interacting reagents have been optimized simultaneously (coöptimization) utilizing response surface designs coupled with computer analysis of the data. Reagent efficiency is high and analytical performance reliable.

Preparation and Properties of a Cholesterol Oxidase from Nocardia sp. and Its Application to the Enzymatic Assay of Total Cholesterol in Serum

1973 ◽  
Vol 19 (12) ◽  
pp. 1350-1356 ◽  
Author(s):  
W Richmond
Keyword(s):  
Hydrogen Peroxide ◽  
Total Cholesterol ◽  
Enzyme Assay ◽  
Cholesterol Oxidase ◽  
Xylenol Orange ◽  
Extraction And Purification ◽  
Production Of Hydrogen ◽  
Nocardia Sp ◽  
Wide Ph Range ◽  
Ph Range

Abstract I describe the characterization, extraction, and purification of a cholesterol:oxygen oxidoreductase (EC 1.1.3.6) from Nocardia sp. This enzyme catalyzes oxidation of cholesterol to Δ4-choIestenone, with production of hydrogen peroxide. It is very stable, active over a wide pH range, and has a Km of 1.4 x 10-5 mol/ liter. It is highly specific for Δ4- or Δ5-3β-hydroxycholestanes, and may be applied to the assay of serum total cholesterol. In the procedure presented here, hydrogen peroxide is measured by reaction with quadrivalent titanium and xylenol orange. This constitutes a one-enzyme assay with stable reagents, which does not require protein precipitation and is not subject to interference from hemoglobin or bilirubin.

Chronic intake of valproic acid decreases total cholesterol level in adult population

2021 ◽  
Vol 15 (7) ◽  
pp. 1665-1667
Author(s):  
Rao S Aziz ◽  
Usman Saeed ◽  
Liaqat Ali ◽  
Muhammad Arshad ◽  
Roman Abbas ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Total Cholesterol ◽  
Cholesterol Oxidase ◽  
Total Cholesterol Level ◽  
Adult Population ◽  
Venous Blood ◽  
Case Group ◽  
Long Duration ◽  
And Control

Background: Valproic acid (VA) serve as the antimigraine, anti-mental disturbances agent and antiepileptic medicine. After using VA, metabolic rearrangements seen in patients that include alteration in lipoproteins levels; however, this topic is still under discussion and disputable. Aim: To discuss the effects of VA after using for long duration on total levels of cholesterol in adult. Methods: About Eighty candidates participated and they were divided into two groups namely, case group (40 candidates) and control groups40 candidates). All the candidates were asked for collection of venous blood sample in order to determine total cholesterol serum level among them via aid of enzymatic cholesterol oxidase phenol 4-aminoantipyrine peroxidase. By the aid of the logistic regression analysis, the relationship of the long-term VA treatment and the level of total cholesterol was obtained. With respect to our analysis, there is a co relation between total levels of cholesterol and long term usage of VA (P=0.003). Results: There were no noteworthy link for age, sex, body BMI, height medication usage, smoking, diabetes mellitus and VA dosage , except combination of anti-epileptic drugs Conclusion: In a net shell, by using VA for long duration, the total level of cholesterol in adults reduces. Keywords: Lipid profile, side effects, total cholesterol, valproic acid

scholarly journals Mediator-free biosensor using chitosan capped CdS quantum dots for detection of total cholesterol

RSC Advances ◽  
2015 ◽  
Vol 5 (57) ◽  
pp. 45928-45934 ◽  
Author(s):  
Hemant Dhyani ◽  
Md. Azahar Ali ◽  
Satyendra P. Pal ◽  
Saurabh Srivastava ◽  
Pratima R. Solanki ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Total Cholesterol ◽  
Cadmium Sulfide ◽  
Surface Functionalization ◽  
Cholesterol Oxidase ◽  
Cholesterol Esterase ◽  
Cds Quantum Dots ◽  
Oxidase Enzyme ◽  
Enzyme Molecules

An electrochemical mediator-free biosensor platform have been fabricated using in situ synthesized cadmium sulfide quantum dots embedded in chitosan via surface functionalization of cholesterol esterase and cholesterol oxidase enzyme molecules.

Enzymic assay of total cholesterol involving chemical or enzymic hydrolysis--a comparison of methods.

1979 ◽  
Vol 25 (6) ◽  
pp. 976-984 ◽  
Author(s):  
A C Deacon ◽  
P J Dawson
Keyword(s):  
Total Cholesterol ◽  
Potassium Hydroxide ◽  
Cholesterol Oxidase ◽  
Cholesterol Concentration ◽  
Cholesteryl Esters ◽  
Enzymic Hydrolysis ◽  
Hydrolysis Method ◽  
Chemical Hydrolysis ◽  
Incomplete Removal ◽  
Enzymic Assay

Abstract Manual procedures for estimating serum total cholesterol by use of cholesterol oxidase (EC 1.1.3.6) and the phenol--aminophenazone--peroxidase chromogenic system are described, in which cholesteryl esters are hydrolyzed either by use of pancreatic cholesterol ester hydrolase (EC 3.1.1.13) or saponification by ethanolic potassium hydroxide. Both methods are linear up to a cholesterol concentration of 12 mmol/L and are reproducible (between-run CV, about 1.1%). The chemical hydrolysis method yields results that are about 10% lower than those obtained by enzymic hydrolysis, because of incomplete removal of interfering thiols generated during the saponification of serum. The chemical hydrolysis procedure is much less susceptible to interferences, particularly by bilirubin, but the enzymic hydrolysis system is simpler to perform and therefore has a greater potential for mechanization.

Simple enzymatic determination of total cholesterol in gallstones.

1989 ◽  
Vol 35 (11) ◽  
pp. 2247-2249 ◽  
Author(s):  
J S Wei ◽  
H M Huang ◽  
W C Shyu ◽  
C S Wu
Keyword(s):  
Total Cholesterol ◽  
Dimethyl Sulfoxide ◽  
Organic Solvents ◽  
Cholesterol Oxidase ◽  
Enzymatic Activities ◽  
Simple Method ◽  
Spectroscopic Measurements ◽  
Assay Mixture ◽  
Enzymatic Determination

Abstract In this simple method for rapid enzymatic determination of total cholesterol in human gallstones, gallstone powder is dissolved in an 80/20 by vol mixture of N,N-dimethylformamide and dimethyl sulfoxide and reacted directly with the aqueous enzymatic reagent, without further treatment. The organic solvents do not interfere with enzymatic activities of cholesterol oxidase, esterase, and peroxidase in the assay mixture. The method is reproducible (CVs 3.7% to 6.6%), analytical recoveries ranged from 98.6% to 102%, and linearity is good. Furthermore, results correlated well with those obtained by infrared spectroscopic measurements.

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