Design and Characterization of Targeted Ultrasound Microbubbles for Diagnostic Use

2011 ◽  
Vol 37 (1) ◽  
pp. 136-150 ◽  
Author(s):  
Astrid Hilde Myrset ◽  
Hege Brincker Fjerdingstad ◽  
Ragnar Bendiksen ◽  
Bente Elisabeth Arbo ◽  
Roger Malerbakken Bjerke ◽  
...  
Keyword(s):  
Diagnostic Use ◽  
Targeted Ultrasound ◽  
Ultrasound Microbubbles

Myeloperoxidase deficiency in dogs observed with the ADVIA®120

2010 ◽  
Vol 38 (03) ◽  
pp. 139-146 ◽  
Author(s):  
J. Richartz ◽  
N. Bauer ◽  
A. Moritz ◽  
S. Klenner
Keyword(s):  
Screening Tool ◽  
High Standard ◽  
Pancreatic Abscess ◽  
High Standard Deviation ◽  
Diagnostic Use ◽  
Cellular Volume ◽  
Myeloperoxidase Deficiency ◽  
Scatter Plots ◽  
The Mean

Summary Objective: In contrast to humans, neutrophil myeloperoxidase deficiency (MPOD) has been rarely investigated in dogs. The hematology analyzer ADVIA®120 differentiates leukocytes based on the cellular volume and their myeloperoxidase concentration. The aim of this study was the characterization of myeloperoxidase deficiency in dogs and the evaluation of the diagnostic use of the ADVIA®120 Myeloperoxidase Index (MPXI). Material and methods: ADVIA® peroxidase scatter plots indicative of MPOD were reviewed. Severity of MPOD was classified semiquantitatively in three groups (MPOD grade 1–3): MPOD grade 1 (MPOD-1): neutrophils showing an abnormal shift of the population, < 25% extending in the monocyte cluster and therefore misclassified, MPOD-2: ~25–50% of neutrophils misclassified, MPOD-3: 50–100% of the neutrophils misclassified due to their location in the monocyte cluster. Sex, age, and breed of the dogs as well as diagnosis, and MPXI were recorded. Results: 29 dogs (nine females and 20 males belonging to 23 breeds) with 38 analyses consistent with MPOD were found. Diseases were characterized by severe leukocyte consumption and included mainly parvovirosis (8/29), DIC/sepsis (3/29), pyometra, pyothorax, pneumonia, pancreatic abscess, and cystitis. A significantly lower mean MPXI in MPOD-3 was present in comparison to the mean MPXI of MPOD-1 (p < 0.05), however, there was a great overlap between the groups. Conclusion: Diseases associated with neutrophil consumption may show an acquired MPOD in dogs. High standard deviation limits the diagnostic use of the MPXI for detection of MPOD. Clinical relevance: The ADVIA®120 cytograms are a good screening tool for detection of MPOD in dogs, but the use of the MPXI is impaired in this species.

scholarly journals Anti-inflammatory and Anti-thrombotic Efficacy of Targeted Ultrasound Microbubbles on LPS-induced HUVEC Cells

2021 ◽  
Vol 41 (10) ◽  
pp. 4761-4769
Author(s):  
JINDONG SUN ◽  
SHIJUN PAN ◽  
HUAMIN YU ◽  
HAIQIANG HU ◽  
YU SUN ◽  
...  
Keyword(s):  
Targeted Ultrasound ◽  
Huvec Cells ◽  
Anti Inflammatory ◽  
Ultrasound Microbubbles

RGD-Tagged Microbubbles Generated by Versatile Fabrication Protocols for In Vitro Cell Targeting and In Vivo Mouse Imaging of Tumor Vascularization

2020 ◽  
Vol 12 (5) ◽  
pp. 725-732
Author(s):  
Fangfang Yu ◽  
Jiaping Li ◽  
Yan Yang ◽  
Yueyue Cheng ◽  
Xianwei Ni ◽  
...  
Keyword(s):  
Malignant Tumors ◽  
Ultrasound Contrast Agents ◽  
Cell Targeting ◽  
Tumor Vascularization ◽  
Targeted Ultrasound ◽  
One Step ◽  
Functional Biomaterials ◽  
Ultrasound Microbubbles

By using an organic framework to fabricate functional biomaterials, novel design and advanced biomedical applications of polymeric microbubbles for in vivo targeting and disease-oriented imaging of tumor vascularization can be developed. The present study describes novel synthetic protocols to fabricate RGD (Arginine-Glycine-Aspartic)-tagged αvβ3-targeted ultrasound microbubbles. The microbubbles were synthesized by emulsion polymerization techniques. Two types of microbubbles (MBs-1 and MBs-2) were obtained via biotin-streptavidin conjugation to poly(butyl cyanoacrylate) microbubbles (MBs-0) obtained by one-step synthesis in reverse order. The size distributions and surface zeta potentials were characterized. The results showed that the sizes of the MBs-2 were larger than that those of the MBs-1, and the MBs-2 showed decreased charge compared to MBs-1. In cell targeting studies, MBs-2 exhibited relatively stronger targeting affinity for αvβ3 integrins, while MBs-1 showed weaker targeting capability. Furthermore, in vivo mice imaging using MBs-2 for intravenous injection exhibited an obvious and sustained signal increase, which revealed the accumulative of MBs-2 anchoring in tumor. Hence, MBs-2 have been proven to be a promising candidate for using as ultrasound contrast agents for the early diagnosis of αvβ3-overexpressing malignant tumors, including breast cancer.

Acoustic and optical characterization of targeted ultrasound contrast agents

2013 ◽  
Vol 133 (5) ◽  
pp. 3261-3261
Author(s):  
Camilo Perez ◽  
Jarred Swalwell ◽  
Juan Tu ◽  
Hong Chen ◽  
Andrew Brayman ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Optical Characterization ◽  
Ultrasound Contrast Agents ◽  
Ultrasound Contrast ◽  
Targeted Ultrasound

scholarly journals Adding the merits of contrast to the ease of mammography; can we highlight what’s behind breast asymmetries?

2019 ◽  
Vol 50 (1) ◽  
Author(s):  
Rasha Mohamed Kamal ◽  
Amr Farouk Ibrahim Moustafa ◽  
Sherihan Fakhry ◽  
Eman Faker Kamal ◽  
Amira Radwan ◽  
...  
Keyword(s):  
High Energy ◽  
Imaging Tool ◽  
Targeted Ultrasound ◽  
Ultrasound Study ◽  
Contrast Enhanced ◽  
Breast Asymmetries ◽  
Diagnostic Work ◽  
Work Up ◽  
Diagnostic Work Up

Abstract Background Breast symmetry is one of the major things that radiologists assess when looking at mammograms and is one of the most challenging mammographic findings to evaluate. Contrast-enhanced spectral mammography (CESM) is an emerging mammography technique that has shown comparable sensitivity and specificity to MRI. The purpose of this study is to assess the value of CESM in characterization of breast asymmetries (BAs) and if it should be incorporated in its diagnostic work-up. Results Three hundred sixty-five patients with mean age of 47 years were included in the study. CESM was performed aiming for characterization of 380 suspicious or indeterminate breast asymmetries. Assessment of subtracted high-energy images (HEI) markedly improves the overall accuracy reaching 88.4%. Further improvement of the overall accuracy was achieved on combined assessment of the low-energy images (LEI), subtracted high-energy images (HEI), and ultrasound reaching 91.3%. Conclusion CESM is considered as a valuable complementary imaging tool considering the evaluation of breast asymmetries and should be incorporated in its diagnostic work-up in cases not resolved on an initial combined mammography and targeted ultrasound study especially in the presence of a heterogeneous dense breast parenchyma. Yet, this may be hindered in the presence of inflammatory signs because of the overlapping imaging criteria.

scholarly journals Development of prostate specific membrane antigen targeted ultrasound microbubbles using bioorthogonal chemistry

PLoS ONE ◽  
2017 ◽  
Vol 12 (5) ◽  
pp. e0176958 ◽  
Author(s):  
Aimen Zlitni ◽  
Melissa Yin ◽  
Nancy Janzen ◽  
Samit Chatterjee ◽  
Ala Lisok ◽  
...  
Keyword(s):  
Prostate Specific Membrane Antigen ◽  
Bioorthogonal Chemistry ◽  
Targeted Ultrasound ◽  
Ultrasound Microbubbles ◽  
Specific Membrane

CLONING AND CHARACTERIZATION OF THE LEISHMANIA (VIANNIA) BRAZILIENSIS HSP70 GENE. DIAGNOSTIC USE OF THE C-TERMINAL FRAGMENT rLb70(513–663)

2003 ◽  
Vol 89 (2) ◽  
pp. 372-378 ◽  
Author(s):  
Ada I. Zurita ◽  
Javier Rodríguez ◽  
José E. Piñero ◽  
Rosa Pacheco ◽  
Emma Carmelo ◽  
...  
Keyword(s):  
Hsp70 Gene ◽  
Diagnostic Use ◽  
Terminal Fragment
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