scholarly journals Impact of Time out of Intended Storage and Freeze-Thaw Rates on the Stability of Adeno-Associated Virus 8 and 9

Author(s):  
Jared S. Bee ◽  
Yu (Zoe) Zhang ◽  
Megan Kuhn Phillippi ◽  
Sheyla Finkner ◽  
Tesfu Mezghebe ◽  
...  
2010 ◽  
Vol 163-167 ◽  
pp. 3297-3300 ◽  
Author(s):  
Jia Wei Shi ◽  
Hong Zhu ◽  
Zhi Shen Wu ◽  
Gang Wu

Coupon tests were conducted to investigate the mechanical characteristics of basalt FRP (BFRP) sheet, basalt-carbon hybrid FRP sheets and the corresponding epoxy rein under the effect of freeze-thaw cycling. FRP sheets and epoxy rein coupons were subjected to up to 200 and 250 freeze-thaw cycles respectively. Test parameters included the number of freeze-thaw cycles and the types of FRP composites. Test results show that (1) BFRP sheet perform better than CFRP or GFRP sheets under high freeze-thaw cycles; (2) exposed hybrid FRP sheets not only show very little loss in mechanical properties, but also contribute to the stability of test data; (3) mechanical properties of rein epoxy decrease significantly with increasing freeze-thaw cycles.


Viruses ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 668 ◽  
Author(s):  
Bridget Lins-Austin ◽  
Saajan Patel ◽  
Mario Mietzsch ◽  
Dewey Brooke ◽  
Antonette Bennett ◽  
...  

Adeno-associated viruses (AAVs) are small, non-pathogenic ssDNA viruses being used as therapeutic gene delivery vectors for the treatment of a variety of monogenic diseases. An obstacle to successful gene delivery is inefficient capsid trafficking through the endo/lysosomal pathway. This study aimed to characterize the AAV capsid stability and dynamics associated with this process for a select number of AAV serotypes, AAV1, AAV2, AAV5, and AAV8, at pHs representative of the early and late endosome, and the lysosome (6.0, 5.5, and 4.0, respectively). All AAV serotypes displayed thermal melt temperatures that varied with pH. The stability of AAV1, AAV2, and AAV8 increased in response to acidic conditions and then decreased at pH 4.0. In contrast, AAV5 demonstrated a consistent decrease in thermostability in response to acidification. Negative-stain EM visualization of liposomes in the presence of capsids at pH 5.5 or when heat shocked showed induced remodeling consistent with the externalization of the PLA2 domain of VP1u. These observations provide clues to the AAV capsid dynamics that facilitate successful infection. Finally, transduction assays revealed a pH and temperature dependence with low acidity and temperatures > 4 °C as detrimental factors.


Author(s):  
M. Lessard ◽  
M. Baalbaki ◽  
P.-C. Aïtcin

The stability of the air content of concrete during pumping has been the subject of a number of recent investigations. Because increasing volumes of concrete are placed with the aid of pumps and the durability of such concrete to freezing and thawing (ASTM C666) as well as the scaling resistance (ASTM C672) preoccupy engineers, a study concerning the stability of the air-void system of a concrete with 45 to 50 MPa compressive strength was carried out. The slump of the three tested concretes ranged between 85 and 115 mm. Three pumping setups were studied. In the first, the concrete was pumped horizontally; in the second the concrete was pumped upward and then downward. In the third, the vertical setup was used but a reduced section was placed at the end of the pump line, and the concrete was allowed to free fall a short distance. For each pump setup, the concrete was sampled before being placed in the pump and after leaving the pump. The results clearly show that when the concrete is pumped horizontally, the spacing factor (L) and the specific surface of the air-void system are barely altered. On the other hand, after pumping the concrete vertically without a reduced end section, it was impossible to obtain an L less than 230 μm, the maximum spacing factor allowed by Canadian standards (CSA A23.1) to ensure good frost durability. Furthermore, the specific surface of the air bubbles fell to 20 mm−1, which is inferior to the 25-mm−1 value recommended in Canadian standards. By placing a reduced section at the end of the vertical pump line, it was possible to enhance the air-void system but that procedure still fell short of ensuring a system that satisfies the air-void system recommended by Canadian standards to ensure proper frost durability. Although the pumped concrete mixtures did not always satisfy the requirements of CSA A23.1 regarding air-void systems, they satisfied the requirements of ASTM C666 (Procedure A) for resistance to freeze-thaw cycles. Freeze-thaw resistance in the presence of deicing salts was evaluated according to ASTM C672. After 50 frost cycles, all but one concrete exhibited mass losses that were lower than the maximum permissible limit of 0.50 kg/m2 required by BNQ 2621-900, the standard currently enforced in the province of Quebec. Placing a reduced section at the end of the pump line creates a light counterpressure in the descending section of the pump line, which allows the conservation of an acceptable air-void system. Considering the appreciable improvement in the preservation of air-void characteristics when a reduced section was placed at the end of the pump line, it was decided to proceed with further experimental work using four 90-degree elbows placed at the end of the vertically hanging pump line.


2003 ◽  
Vol 8 (2) ◽  
pp. 210-215 ◽  
Author(s):  
Barbara A. Kozikowski ◽  
Thomas M. Burt ◽  
Debra A. Tirey ◽  
Lisa E. Williams ◽  
Barbara R. Kuzmak ◽  
...  

A diverse set of 320 compounds from the Procter & Gamble Pharmaceuticals organic compound repository was prepared as 20-mM DMSO solutions and stored at 4°C under argon in pressurized canisters to simulate a low-humidity environment. The plates were subjected to 25 freeze/thaw cycles while being exposed to ambient atmospheric conditions after each thaw to simulate the time and manner by which compound plates are exposed to the atmosphere during typical liquid-handling and high-throughput screening processes. High-performance liquid chromatography–mass spectrometry with evaporative light-scattering detection was used to quantitate the amount of compound remaining after every 5th freeze/thaw cycle. Control plates were stored either at room temperature under argon or at 4°C under argon without freeze/thaw cycling and were evaluated at the midpoint and the endpoint of the study. The study was conducted over a short time period (i.e., 7 weeks) to minimize the effect of compound degradation over time due to the exposure of the compounds to DMSO.The results from this study will be used to determine the maximum number of freeze/thaw cycles that can be achieved while maintaining acceptable compound integrity.(Journal of Biomolecular Screening 2003:210-215)


2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Yuxia Zhao ◽  
Jun Feng ◽  
Kangqi Liu ◽  
Hongwei Xu ◽  
Liqun Wang ◽  
...  

Due to the threat of global warming and the accelerated melting of glaciers and permafrost, the stability of slopes in permafrost regions has received an increasing amount of attention from scholars. However, research on the stability of soil-rock road cutting slopes in high-latitude and low-altitude permafrost regions of the Greater Khingan Mountains in the Inner Mongolia Autonomous Region has not been reported. For this reason, a study of the stability of a slope with a high ice content in section K105 + 600 to K105 + 700 of National Highway 332 is conducted. The slope is 20 m high and the slope angle is 45°, and the risk of landslides on this slope under the action of freeze-thaw erosion is very high. Because of this, field in situ monitoring, indoor freeze-thaw tests, thermal parameter tests, and ABAQUS numerical simulation models are used to study the stability of the slope. After collecting the continuous temperature, moisture, settlement, and slope deformation data, it was found that the slope was undergoing dynamic changes. The creep of shallow slopes increased with the number of freeze-thaw cycles. After approximately 150 freeze-thaw cycles, the slope safety factor was less than 1, which means that the slope had reached the limit equilibrium state. Therefore, freeze-thaw erosion greatly reduced the stability of the slope. Hence, the stability of the slope must be protected during its entire life cycle. This study provides a reference for the design and construction of road cutting slopes in the high-latitude and low-altitude permafrost regions of the Greater Khingan Mountains.


2013 ◽  
Vol 333-335 ◽  
pp. 1867-1871
Author(s):  
Hui Mei Zhang ◽  
Hai Bo Cao ◽  
Xiang Miao Xie

The stability of tunnel surrounding rock is a key scientific issue in the design and construction of the tunnel engineering. Taking the load pattern of incremental form, the distribution law of stress and displacement field of the Osaka mountain tunnel after excavation was calculated based on the D-P yield criterion; Basing on the comprehensive heat and mass transfer theory, the temperature field in the surrounding rock was calculated and the variation of stress and displacement field after the surrounding rock experiencing the free-thaw cycles. All of the results can provide some references for the design and safe construction of the tunnel engineering under the action of freeze-thaw environment in cold regions.


RSC Advances ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 4731-4741 ◽  
Author(s):  
Chunxia Su ◽  
Younan Feng ◽  
Jing Ye ◽  
Yuemei Zhang ◽  
Zhiming Gao ◽  
...  

For the first time sodium alginate is used to improve the stability of oil body emulsions against salt, pH and freeze–thaw cycling.


2021 ◽  
Author(s):  
Cian Holohan ◽  
Sophia Hanrahan ◽  
Nathan Feely ◽  
Peng Li ◽  
John O'Connell ◽  
...  

The events of the last year have highlighted the complexity of implementing large-scale molecular diagnostic testing for novel pathogens. The purpose of this study was to determine the chemical influences of sample collection media and storage on the stability and detection of viral nucleic acids by qRT-PCR. We studied the mechanism(s) through which viral transport media (VTM) and number of freeze-thaw cycles influenced the analytical sensitivity of qRT-PCR detection of SARS-CoV-2. Our goal is to reinforce testing capabilities and identify weaknesses that could arise in resource-limited environments that do not have well-controlled cold chains. The sensitivity of qRT-PCR analysis was studied in four VTM for synthetic single-stranded RNA (ssRNA) and double-stranded DNA (dsDNA) simulants of the SARS-CoV-2 genome. The sensitivity and reproducibility of qRT-PCR for the synthetic ssRNA and dsDNA were found to be highly sensitive to VTM with the best results observed for ssRNA in HBSS and PBS-G. Surprisingly, the presence of epithelial cellular material with the ssRNA increased the sensitivity of the qRT-PCR assay. Repeated freeze-thaw cycling decreased the sensitivity of the qRT-PCR with two noted exceptions. The choice of VTM is critically important to defining the sensitivity of COVID-19 molecular diagnostics assays and this study suggests they can impact upon the stability of the SARS-CoV-2 viral genome. This becomes increasingly important if the virus structure is destabilised before analysis, which can occur due to poor storage conditions. This study suggests that COVID-19 testing performed with glycerol-containing PBS will produce a high level of stability and sensitivity. These results are in agreement with clinical studies reported for patient-derived samples.


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