scholarly journals Retraction notice to “Knockdown of long non-coding RNA CCAT1 suppresses proliferation and EMT of human cervical cancer cell lines by down-regulating Runx2” [Experimental and Molecular Pathology 113 (2020) 104380]

Author(s):  
Ruiping Li ◽  
Jinyu Liu ◽  
Jinhong Qi
Marine Drugs ◽  
2019 ◽  
Vol 17 (5) ◽  
pp. 256 ◽  
Author(s):  
Yiqiao Liu ◽  
Jiang Qian ◽  
Zhihua Sun ◽  
Dongting Zhangsun ◽  
Sulan Luo

Nicotinic acetylcholine receptors (nAChRs) are associated with various cancers, but the relation between nAChRs and cervical cancer remains unclear. Therefore, this study investigated the differential expression of nAChR subunits in human cervical cancer cell lines (SiHa, HeLa, and CaSki) and in normal ectocervical cell lines (Ect1/E6E7) at mRNA and protein levels. Two specific nAChR subtype blockers, αO-conotoxin GeXIVA and α-conotoxin TxID, were then selected to treat different human cervical cancer cell lines with specific nAChR subtype overexpression. The results showed that α3, α9, α10, and β4 nAChR subunits were overexpressed in SiHa cells compared with that in normal cells. α9 and α10 nAChR subunits were overexpressed in CaSki cells. α*-conotoxins that targeted either α9α10 or α3β4 nAChR were able to significantly inhibit cervical cancer cell proliferation. These findings may provide a basis for new targets for cervical cancer targeted therapy.


2008 ◽  
Vol 127 ◽  
pp. S147
Author(s):  
Luis Jave-Suarez ◽  
Naela Arreygue-Garcia ◽  
Adriana Aguilar-Lemarrroy ◽  
Miriam Jimenez-Perez ◽  
Angel Arregui ◽  
...  

2018 ◽  
Vol 37 (4) ◽  
pp. 602-615 ◽  
Author(s):  
Viviane A. O. Silva ◽  
Ana Laura V. Alves ◽  
Marcela N. Rosa ◽  
Larissa R. V. Silva ◽  
Matias E. Melendez ◽  
...  

2014 ◽  
Vol 92 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Aline Beckenkamp ◽  
Danielle Bertodo Santana ◽  
Alessandra Nejar Bruno ◽  
Luciane Noal Calil ◽  
Emerson André Casali ◽  
...  

Cervical cancer is the third most frequent cancer in women worldwide. Adenine nucleotide signaling is modulated by the ectonucleotidases that act in sequence, forming an enzymatic cascade. Considering the relationship between the purinergic signaling and cancer, we studied the E-NTPDases, ecto-5′-nucleotidase, and E-NPPs in human cervical cancer cell lines and keratinocytes. We evaluated the expression profiles of these enzymes using RT-PCR and quantitative real-time PCR analysis. The activities of these enzymes were examined using ATP, ADP, AMP, and p-nitrophenyl-5′-thymidine monophosphate (p-Nph-5′-TMP) as substrate, in a colorimetric assay. The extracellular adenine nucleotide hydrolysis was estimated by HPLC analysis. The hydrolysis of all substrates exhibited a linear pattern and these activities were cation-dependent. An interesting difference in the degradation rate was observed between cervical cancer cell lines SiHa, HeLa, and C33A and normal imortalized keratinocytes, HaCaT cells. The mRNA of ecto-5′-nucleotidase, E-NTPDases 5 and 6 were detectable in all cell lines, and the dominant gene expressed was the Entpd 5 enzyme, in SiHa cell line (HPV16 positive). In accordance with this result, a higher hydrolysis activity for UDP and GDP nucleotides was observed in the supernatant of the SiHa cells. Both normal and cancer cells presented activity and mRNAs of members of the NPP family. Considering that these enzymes exert an important catalytic activity, controlling purinergic nucleotide concentrations in tumors, the presence of ectonucleotidases in cervical cancer cells can be important to regulate the levels of extracellular adenine nucleotides, limiting their effects.


2016 ◽  
Vol 242 (2) ◽  
pp. 184-193 ◽  
Author(s):  
Tawin Iempridee

Long non-coding RNA H19 is aberrantly expressed in multiple malignancies and its expression levels correlate with recurrence, metastasis, and patient survival. Despite numerous reports documenting the role of H19 in carcinogenesis, its contribution to cervical cancer development is still largely unknown. In this study, I observed that H19 expression was elevated in cervical cancer cell lines and could be detected in extracellular vesicles in the culture medium. In addition, I demonstrated, by overexpression and knockdown experiments, that H19 promoted cell proliferation and multicellular tumor spheroid formation without significantly affecting apoptosis and cell migration. Finally, treatment with transforming growth factor beta and hypoxia-mimetic CoCl2 could modulate H19 levels in a cell line-specific manner. These findings indicate that H19 promotes both anchorage-specific and -independent growth of cervical cancer cell lines and may serve as a potential target for cancer diagnosis and therapy.


2006 ◽  
Vol 12 (1) ◽  
pp. 250-256 ◽  
Author(s):  
Christopher M. Lee ◽  
Christa B. Fuhrman ◽  
Vicente Planelles ◽  
Morgan R. Peltier ◽  
David K. Gaffney ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document