Smooth and robust multi-mode shaped commands

2022 ◽  
Vol 168 ◽  
pp. 108658
Author(s):  
Abdullah Alshaya ◽  
Khaled Alhazza
Keyword(s):  
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.


2009 ◽  
Vol E92-B (12) ◽  
pp. 3717-3725
Author(s):  
Thomas HUNZIKER ◽  
Ziyang JU ◽  
Dirk DAHLHAUS

2014 ◽  
Vol E97.C (7) ◽  
pp. 781-786 ◽  
Author(s):  
Mohammad NASIR UDDIN ◽  
Takaaki KIZU ◽  
Yasuhiro HINOKUMA ◽  
Kazuhiro TANABE ◽  
Akio TAJIMA ◽  
...  

2020 ◽  
Vol 1099 ◽  
pp. 16-25 ◽  
Author(s):  
Daniel Rosenkranz ◽  
Fabian L. Kriegel ◽  
Emmanouil Mavrakis ◽  
Spiros A. Pergantis ◽  
Philipp Reichardt ◽  
...  

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