scholarly journals Long chain fatty acid binding to human plasma albumin.

1975 ◽  
Vol 250 (6) ◽  
pp. 2333-2338
Author(s):  
JD Ashbrook ◽  
AA Spector ◽  
EC Santos ◽  
JE Fletcher
Keyword(s):  
Fatty Acid ◽  
Human Plasma ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
Plasma Albumin ◽  
Fatty Acid Binding ◽  
Long Chain

scholarly journals The effect of intracellular pH on long-chain fatty acid uptake in 3T3-L1 adipocytes: evidence that uptake involves the passive diffusion of protonated long-chain fatty acids across the plasma membrane

1996 ◽  
Vol 313 (2) ◽  
pp. 487-494 ◽  
Author(s):  
Bernardo L. TRIGATTI ◽  
Gerhard E. GERBER
Keyword(s):  
Fatty Acids ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acids ◽  
Cytoplasmic Ph ◽  
Long Chain Fatty Acid ◽  
Intact Cells ◽  
Fatty Acid Binding ◽  
Long Chain

To understand the mechanism of long-chain fatty acid permeation of the plasma membrane in mammalian cells, the effects of changes in the cytoplasmic pH on the internalization of physiologically relevant, submicromolar concentrations of uncomplexed long-chain fatty acids were investigated in 3T3-L1 adipocytes. The acidification of the cytoplasm upon NH4Cl prepulsing of intact cells was accompanied by a rapid reduction of cellular long-chain fatty acid uptake (measured as the total accumulation of [9,10-3H]oleate). This was followed by a slow recovery to normal levels of uptake as the cytoplasmic pH recovered. Conventional filtration assays do not distinguish between fatty acid movement across the plasma membrane and intracellular steps, such as binding to cytoplasmic fatty acid-binding proteins or metabolism. While the in vitro binding of a photoreactive fatty acid, 11-m-diazirinophenoxy[11-3H]undecanoate, to a cytoplasmic fatty acid-binding protein was insensitive to changes in pH from pH 7.5 to 5.5, the in vitro conversion of oleate into oleoyl-CoA by cellular acyl-CoA synthetase decreased dramatically. Therefore, the labelling of the 15 kDa cytoplasmic fatty acid-binding protein in intact cells by the photoreactive fatty acid was used as a more direct measure of the permeation of the probe across the plasma membrane. Acidification of the cytoplasm resulted in an immediate reduction in the labelling of this protein in intact adipocytes. Its photolabelling recovered, however, upon the recovery of the cytoplasmic pH to normal levels. This was due to effects of the cytoplasmic pH on the permeation of the photoreactive fatty acid across the plasma membrane rather than its binding to the 15 kDa protein or metabolism in vivo. This is the first demonstration that the movement of physiologically relevant, submicromolar concentrations of uncomplexed long-chain fatty acids across the plasma membrane of intact cells is coupled to the cytoplasmic pH and suggests that it occurs by the diffusion of the protonated long-chain fatty acid through the lipid bilayer.

Long-chain fatty acid-binding to albumin: re-evaluation with directly measured concentrations

1994 ◽  
Vol 1215 (3) ◽  
pp. 321-326 ◽  
Author(s):  
Horst Rose ◽  
Martina Conventz ◽  
Yvan Fischer ◽  
Eberhard Jüngling ◽  
Thomas Hennecke ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
Fatty Acid Binding ◽  
Long Chain
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