Human schistosomiasis mansoni: intensity of infection differentially affects the production of interleukin-10, interferon-$gamma; and interleukin-13 by soluble egg antigen or adult worm antigen stimulated cultures

The circumoval precipitin test (COPT), enzyme-linked immunosorbent assay (ELISA) and the immunoblotting anti-adult worm antigen (AWA) and soluble egg antigen (SEA) tests were applied to 17 chronically schistosome-infected patients for the detection of anti-Schistosoma mansoni antibodies before and on four occasions after oxamniquine administration over a period of six months. Compared to a control group, schistosomiasis patients showed high levels of IgG antibodies in AWA and SEA-ELISA. A decrease in IgG levels was observed six months after treatment, although negative reactions were not obtained. Significant decreases in IgG1, IgG3 and, mainly, IgG4, but not anti-SEA IgG2 levels were observed six months after treatment, again without negativity. Analysis of anti-AWA IgG antibodies by immunoblotting before treatment showed a 31 kDa strand in 14 patients (82%) which disappeared in three cases up to six months after treatment; furthermore, anti-SEA IgG antibodies showed the same band in nine patients (53%) before treatment, which disappeared in only four cases up to six months after treatment.

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Interleukin-13 in the skin and interferon-gamma in the liver are key players in immune protection in human schistosomiasis

Immunological Reviews ◽

10.1111/j.0105-2896.2004.00195.x ◽

2004 ◽

Vol 201 (1) ◽

pp. 180-190 ◽

Cited By ~ 42

Author(s):

Alain Dessein ◽

Bourema Kouriba ◽

Carole Eboumbou ◽

Helia Dessein ◽

Laurent Argiro ◽

...

Keyword(s):

Interferon Gamma ◽

Immune Protection ◽

Interleukin 13 ◽

Human Schistosomiasis ◽

Key Players

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Studies on the production and regulation of interleukin, IL-13, IL-4 and interferon-gamma in human Schistosomiasis mansoni

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762002000900023 ◽

2002 ◽

Vol 97 (suppl 1) ◽

pp. 113-114 ◽

Cited By ~ 4

Author(s):

Clarice N Lins de Morais ◽

Joelma Rodrigues de Souza ◽

Wlademir Gomes de Melo ◽

Marcílio L Aroucha ◽

Ana Lúcia C Domingues ◽

...

Keyword(s):

Interferon Gamma ◽

Schistosomiasis Mansoni ◽

Human Schistosomiasis

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Interferon gamma-mediated toxoplasmastasis in human macrophages is not abrogated by interleukin-10

Immunology Letters ◽

10.1016/s0165-2478(97)88587-2 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 430-431

Author(s):

C MacKenzie

Keyword(s):

Interferon Gamma ◽

Interleukin 10 ◽

Human Macrophages

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Immunoprecipitins in human schistosomiasis detected with adult worm antigens released by 3M KC1

Journal of Helminthology ◽

10.1017/s0022149x00007276 ◽

1977 ◽

Vol 51 (1) ◽

pp. 71-72 ◽

Cited By ~ 3

Author(s):

Mauro Scapin ◽

Miriam Tendler

Keyword(s):

Schistosoma Mansoni ◽

Adult Worm ◽

Calcium Chloride ◽

Surface Proteins ◽

Immunochemical Characterization ◽

Human Schistosomiasis

Several methods are being applied for solubilizing adult Schistosoma mansoni antigens which may allow their immunochemical characterization and purification. Studies on antigens and immunoprecipitins in schistosome infections have been carried out with homogenized extracts of adult worms and/or larval forms of Schistosoma mansoni in saline (Biguet et al., 1962; Kagan and Norman, 1963; Silva and Ferri, 1965), or water (Kent, 1963). Kusel (1972) studied surface proteins in S. mansoni membrane, treating the worms with 0·5% saponin in 3% calcium chloride. Murrel et al. (1974) compared by immunodiffusion a crude culture antigen with extracts obtained by freeze-thawing of adult worms and by treatment in 3M KCl.

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