Mast cells and nerve fibers in interstitial cystitis (IC): An algorithm for histologic diagnosis via quantitative image analysis and morphometry (QIAM)

Urology ◽  
1997 ◽  
Vol 49 (5) ◽  
pp. 41-47 ◽  
Author(s):  
Marietta A. Hofmeister ◽  
Fang He ◽  
Timothy L. Ratliff ◽  
Thomas Mahoney ◽  
Michael J. Becich
Keyword(s):  
Image Analysis ◽  
Mast Cells ◽  
Interstitial Cystitis ◽  
Nerve Fibers ◽  
Quantitative Image Analysis ◽  
Histologic Diagnosis ◽  
Quantitative Image

scholarly journals Dramatic Decrease of Innervation Density in Bone after Ovariectomy

Endocrinology ◽  
2005 ◽  
Vol 146 (1) ◽  
pp. 503-510 ◽  
Author(s):  
B. Burt-Pichat ◽  
M. H. Lafage-Proust ◽  
F. Duboeuf ◽  
N. Laroche ◽  
C. Itzstein ◽  
...  
Keyword(s):  
Image Analysis ◽  
Bone Loss ◽  
Density Measurement ◽  
Nerve Fibers ◽  
Quantitative Image Analysis ◽  
Mineral Density ◽  
Functional Link ◽  
Sciatic Neurectomy ◽  
Quantitative Image ◽  
Neurofilament 200

Recent studies have demonstrated that bone is highly innervated and contains neuromediators that have functional receptors on bone cells. However, no data exist concerning the quantitative changes of innervation during bone loss associated with estrogen withdrawal. To study the involvement of nerve fibers in the regulation of bone remodeling, we have evaluated the modifications of innervation in a classical in vivo model of osteopenia in rats, ovariectomy (OVX). Skeletal innervation was studied by immunocytochemistry using antibodies directed against specific neuronal markers, neurofilament 200 and synaptophysin, and the neuromediator glutamate. Sciatic neurectomy, another model of bone loss due to limb denervation and paralysis, was used to validate our quantitative image analysis technique of immunostaining for nerve markers. Female Wistar rats at 12 wk of age were sham-operated (SHAM) or ovariectomized (OVX). Bone mineral density measurement and bone histomorphometry analysis of tibiae 14 d after surgery demonstrated a significant bone loss in OVX compared with SHAM. We observed an important reduction of nerve profile density in tibiae of OVX animals compared with SHAM animals, whereas innervation density in skin and muscles was similar for OVX and control rats. Quantitative image analysis of immunostainings demonstrated a significant decrease of the percentage of immunolabeling per total bone volume of neurofilament 200, synaptophysin, and glutamate in both the primary and secondary spongiosa of OVX rats compared with SHAM. These data indicate for the first time that OVX-induced bone loss in rat tibiae is associated with a reduction in nerve profile density, suggesting a functional link between the nervous system and the bone loss after ovariectomy.

Digital image acquisition, processing, and analysis in a polymer microscopy laboratory

1994 ◽  
Vol 52 ◽  
pp. 454-455
Author(s):  
Vinod K. Berry ◽  
Xiao Zhang
Keyword(s):  
United States ◽  
Image Analysis ◽  
Digital Image ◽  
Image Acquisition ◽  
Image Transmission ◽  
The United States ◽  
Quantitative Image Analysis ◽  
Quantitative Image

In recent years it became apparent that we needed to improve productivity and efficiency in the Microscopy Laboratories in GE Plastics. It was realized that digital image acquisition, archiving, processing, analysis, and transmission over a network would be the best way to achieve this goal. Also, the capabilities of quantitative image analysis, image transmission etc. available with this approach would help us to increase our efficiency. Although the advantages of digital image acquisition, processing, archiving, etc. have been described and are being practiced in many SEM, laboratories, they have not been generally applied in microscopy laboratories (TEM, Optical, SEM and others) and impact on increased productivity has not been yet exploited as well.In order to attain our objective we have acquired a SEMICAPS imaging workstation for each of the GE Plastic sites in the United States. We have integrated the workstation with the microscopes and their peripherals as shown in Figure 1.

scholarly journals Quantitative image analysis of microbial communities with BiofilmQ

2021 ◽  
Author(s):  
Raimo Hartmann ◽  
Hannah Jeckel ◽  
Eric Jelli ◽  
Praveen K. Singh ◽  
Sanika Vaidya ◽  
...  
Keyword(s):  
Image Analysis ◽  
Microbial Communities ◽  
Dimensional Space ◽  
Three Dimensional ◽  
Software Tool ◽  
Image Cytometry ◽  
Quantitative Image Analysis ◽  
Space And Time ◽  
Quantitative Image ◽  
Three Dimensional Space

AbstractBiofilms are microbial communities that represent a highly abundant form of microbial life on Earth. Inside biofilms, phenotypic and genotypic variations occur in three-dimensional space and time; microscopy and quantitative image analysis are therefore crucial for elucidating their functions. Here, we present BiofilmQ—a comprehensive image cytometry software tool for the automated and high-throughput quantification, analysis and visualization of numerous biofilm-internal and whole-biofilm properties in three-dimensional space and time.

Quantitative Image Analysis of Nuclear Chromatin Distribution for Cytological Diagnosis

2011 ◽  
Vol 55 (5) ◽  
pp. 455-459 ◽  
Author(s):  
Ryotaro Jingu ◽  
Masafumi Ohki ◽  
Sumiko Watanabe ◽  
Sadafumi Tamiya ◽  
Setsuo Sugishima ◽  
...  
Keyword(s):  
Image Analysis ◽  
Quantitative Image Analysis ◽  
Cytological Diagnosis ◽  
Nuclear Chromatin ◽  
Quantitative Image
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