Cellulitis caused by Neisseria meningitidis: the importance of blood cultures

2002 ◽  
Vol 24 (23) ◽  
pp. 180-181
Author(s):  
Joe S. Dylewski
Keyword(s):  
Neisseria Meningitidis ◽  
Blood Cultures

scholarly journals Effects of two blood culture anticoagulants on growth of Neisseria meningitidis

1978 ◽  
Vol 7 (4) ◽  
pp. 332-336
Author(s):  
L Rintala ◽  
H M Pollock
Keyword(s):  
Blood Culture ◽  
Neisseria Meningitidis ◽  
Yeast Extract ◽  
Brain Heart Infusion ◽  
Inhibitory Effect ◽  
Inoculum Size ◽  
Blood Cultures ◽  
Artificial Blood

Tests of 25 strains of Neisseria meningitidis for sensitivity to sodium polyanethol sulfonate (SPS) showed that the sensitivity of strains varied with both inoculum size and SPS concentration. In Trypticase soy broth (TSB), 2 out of 13 strains were sensitive to 0.05% SPS, whereas 8 out of 13 strains were sensitive to the same concentration of SPS in brain heart infusion (BHI). In artificial blood cultures with six strains of meningococci, the addition of 10% defibrinated blood was found to eliminate the sensitivity of all six strains to SPS in BHI, but not of the two strains in TSB. Addition of 1.2% gelatin to artificial blood cultures eliminated the inhibitory effect of 0.05% SPS, whereas the addition of 1% yeast extract to blood cultures containing 0.025% or 0.05% SPS enhanced the inhibitory effect of this anticoagulant. None of the 13 strains tested was inhibited by 0.05% sodium amylosulfate in TSB or BHI alone or in artificial blood cultures with these media.

scholarly journals Gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Neisseria meningitidis in blood culture media

1977 ◽  
Vol 6 (1) ◽  
pp. 1-3 ◽  
Author(s):  
J Eng ◽  
E Holten
Keyword(s):  
Blood Culture ◽  
Neisseria Meningitidis ◽  
Growth Medium ◽  
Solid Medium ◽  
Culture Media ◽  
Inhibitory Effect ◽  
Blood Cultures ◽  
Nutrient Broth

The inhibitory effect of sodium polyanethol sulfonate (0.05%) upon growth of Neisseria meningitidis was found to be neutralized by adding gelatin (l.1%) to the growth medium. The neutralizing effect was demonstrated in solid medium, as well as in nutrient broth for blood cultures. The findings parallel those of Wilkins and West (6) regarding gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Peptostreptococcus anaerobius.

Neisseria meningitidis Bacteremia in Children: Quantitation of Bacteremia and Spontaneous Clinical Recovery Without Antibiotic Therapy

PEDIATRICS ◽  
1987 ◽  
Vol 80 (1) ◽  
pp. 63-67 ◽  
Author(s):  
T. Dennis Sullivan ◽  
Leonard J. LaScolea
Keyword(s):  
Blood Culture ◽  
Antibiotic Therapy ◽  
Neisseria Meningitidis ◽  
Invasive Disease ◽  
Blood Cultures ◽  
Negative Blood Culture ◽  
Clinical Recovery ◽  
Oral Amoxicillin ◽  
Occult Bacteremia ◽  
The Relationship

The relationship between the magnitude of bacteremia due to Neisseria meningitidis and the clinical diagnosis was determined for 43 children who had fever in the presence or absence of focal signs of infection. Bacteremia was quantitated by the previously described procedure using heparinized blood (0.2 to 1.0 mL). Additionally, blood was cultured by means of the radiometric Bactec technique. Seventeen patients had meningitis, 12 had meningococcemia, 13 had unsuspected or "occult" bacteremia, and five had other diagnoses. "Occult" bactermia was diagnosed initially in four patients, but subsequently meningitis was diagnosed. All 13 patients with 500 or more organisms per milliliter had meningitis or meningococcemia in contrast to 12 (55%) of 22 patients with less than 500 organisms per milliliter (P ≤ .0035). Only 18 (42%) of these patients bacteremic with N meningitidis presented with petechiae or purpura. All 13 children with occult bacteremia were sent home after blood cultures were obtained; six of the 13 received a regimen of oral amoxicillin for otitis media. At reexamination (interval 16 to 119 hours) four had meningitis, seven were clinically improved (afebrile, negative blood culture, without invasive disease), and two were still mildly febrile with negative blood culture. Three of these bacteremic children experienced spontaneous clinical and bacteriologic resolution without antibiotic treatment. This has not been previously reported.

Mouse models of infection for Neisseria meningitidis B,2b and Haemophilus influenzae type b diseases

1986 ◽  
Vol 32 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Bernard R. Brodeur ◽  
Peggy S. Tsang ◽  
Josée Hamel ◽  
Yolande Larose ◽  
Serge Montplaisir
Keyword(s):  
Haemophilus Influenzae ◽  
Neisseria Meningitidis ◽  
Inbred Mouse ◽  
Blood Cultures ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Bacterial Strains ◽  
Enhancement Factors ◽  
C57bl Mice

A disseminated and fatal infection was established in C57BL mice, injected intraperitoneally with either Neisseria meningitidis B,2b or Haemophilus influenzae type b bacteria plus enhancement factors. The effects of mucin, hemoglobin, and iron dextran as enhancement of bacterial infectivity in mice were evaluated individually and in combination. A mixture of mucin and hemoglobin was most effective in enhancing the virulence of the pathogens. Inbred mouse lines were more susceptible than outbred ones. Relative virulence of a number of bacterial strains was also compared in one selected mouse line. Neisseria meningitidis B,2b and Haemophilus influenzae type b strains were more virulent than non-B,2b and nontypable strains. Finally, the course of bacteremia for the two infections in mice was followed by quantitative blood cultures. The animals succumbed to the generalized condition within 72 h. In the case of Neisseria meningitidis B,2b, 10 organisms with 4% mucin and 1.6% hemoglobin were sufficient to kill 50% of the animals. For Haemophilus influenzae type b, 300 bacteria with 5% mucin and 2% hemoglobin were necessary to obtain similar effects.

scholarly journals Invasive Potential of Nonencapsulated Disease Isolates of Neisseria meningitidis

2012 ◽  
Vol 80 (7) ◽  
pp. 2346-2353 ◽  
Author(s):  
Kay O. Johswich ◽  
Jianwei Zhou ◽  
Dennis K. S. Law ◽  
Frank St. Michael ◽  
Shannon E. McCaw ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Blood Cultures ◽  
Host Immunity ◽  
Invasive Potential ◽  
Infected Blood ◽  
Content Type ◽  
Acetylneuraminic Acid ◽  
Virulence Phenotype ◽  
Major Virulence Factor ◽  
Rabbit Complement

ABSTRACTThe capsule ofNeisseria meningitidisis the major virulence factor that enables this bacterium to overcome host immunity elicited by complement and phagocytes, rendering it capable of surviving in blood. As such, nonencapsulatedN. meningitidisisolates are generally considered nonpathogenic. Here, we consider the inherent virulence of two nonencapsulatedN. meningitidisisolates obtained from our national surveillance of infected blood cultures in Canada. Capsule deficiency of both strains was confirmed by serology and PCR for thectrAtoctrDgenes andsiaAtosiaCgenes, as well assiaDgenes specific to serogroups B, C, Y, and W135. In both strains, the capsule synthesis genes were replaced by the capsule null locus,cnl-2. In accordance with a lack of capsule, both strains were fully susceptible to killing by both human and baby rabbit complement. However, in the presence of cytidine-5′ monophospho-N-acetylneuraminic acid (CMP-NANA), allowing for lipooligosaccharide (LOS) sialylation, a significant increase of resistance to complement killing was observed. Mass spectrometry of purified LOS did not reveal any uncommon modifications that would explain their invasive phenotype. Finally, in a mouse intraperitoneal challenge model, these nonencapsulated isolates displayed enhanced virulence relative to an isogenic mutant of serogroup B strain MC58 lacking capsule (MC58ΔsiaD). Virulence of all nonencapsulated isolates tested was below that of encapsulated serogroup B strains MC58 and B16B6. However, whereas no mortality was observed with MC58ΔsiaD, 5/10 mice succumbed to infection with strain 2275 and 2/11 mice succumbed to strain 2274. Our results suggest the acquisition of a new virulence phenotype by these nonencapsulated strains.

Neisseria meningitidis serogroup C causing primary polyarthritis in an octogenarian

2020 ◽  
Vol 13 (6) ◽  
pp. e233378 ◽  
Author(s):  
Thomas William Noteman ◽  
Taegyeong Tina Ha ◽  
Elan Micha Tsarfati
Keyword(s):  
Septic Arthritis ◽  
Neisseria Meningitidis ◽  
Blood Cultures ◽  
Joint Fluid ◽  
Age Group ◽  
Edta Blood Sample ◽  
History Of ◽  
The Impact ◽  
Intensive Physiotherapy ◽  
Edta Blood

A man in his 80s presented to the hospital with a 36-hour history of fever, myalgia, bilateral shoulder and right knee pain. Joint fluid aspirates from his shoulders and right knee isolated Gram-negative diplococci. After failing to grow on standard and selective media, Neisseria meningitidis was identified by 16s PCR and subsequently typed as serogroup C. He had no clinical features of meningitis or meningococcaemia. Blood cultures were negative and an EDTA blood sample was negative for meningococcal ctrA gene. Urine PCR was negative for Neisseria gonorrhoeae. He was treated successfully with two arthroscopic joint washouts of his right knee, aspirates of both shoulders, 40 days of intravenous ceftriaxone and intensive physiotherapy as both an inpatient and outpatient. In the literature, we have not found any previously documented cases of serogroup C meningococcus causing polyarticular primary septic arthritis in this age group or guidance on duration of antibiotic treatment. Literature on the impact of rehabilitation to baseline function was also found to be lacking. Although rare, primary meningococcal arthritis (PMA) should be considered as a differential diagnosis in cases of acute polyarticular septic arthritis. Polyarticular PMA in older adults may require prolonged rehabilitation before one might expect to return to premorbid function.

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