β amyloid fragments derived from activated platelets deposit in cerebrovascular endothelium : Usage of a novel blood brain barrier endothelial cell model system

2000 ◽  
Vol 21 ◽  
pp. 220
Author(s):  
Theresa A. Davies ◽  
Heidi J. Long ◽  
Patricia B. Eisenhauer ◽  
Ryan Hastey ◽  
Richard E. Fine ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Cell Model ◽  
Model System ◽  
Brain Barrier ◽  
Activated Platelets ◽  
Blood Brain ◽  
Β Amyloid ◽  
Cell Model System ◽  
Cerebrovascular Endothelium

scholarly journals The effect of nanoparticle size on the probability to cross the blood-brain barrier: an in-vitro endothelial cell model

nano Online ◽  
2016 ◽  
Author(s):  
Malka Shilo ◽  
Anat Sharon ◽  
Koby Baranes ◽  
Menachem Motiei ◽  
Jean-Paul M Lellouche ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Cell Model ◽  
Brain Barrier ◽  
Nanoparticle Size ◽  
Blood Brain

scholarly journals Yuzu and Hesperidin Ameliorate Blood-Brain Barrier Disruption during Hypoxia via Antioxidant Activity

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 843
Author(s):  
Bo Kyung Lee ◽  
Soo-Wang Hyun ◽  
Yi-Sook Jung
Keyword(s):  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Cell Model ◽  
Antioxidant Properties ◽  
Brain Barrier ◽  
Blood Brain ◽  
In Vivo Animal Model ◽  
Bbb Permeability

Yuzu and its main component, hesperidin (HSP), have several health benefits owing to their anti-inflammatory and antioxidant properties. We examined the effects of yuzu and HSP on blood–brain barrier (BBB) dysfunction during ischemia/hypoxia in an in vivo animal model and an in vitro BBB endothelial cell model, and also investigated the underlying mechanisms. In an in vitro BBB endothelial cell model, BBB permeability was determined by measurement of Evans blue extravasation in vivo and in vitro. The expression of tight junction proteins, such as claudin-5 and zonula occludens-1 (ZO-1), was detected by immunochemistry and western blotting, and the reactive oxygen species (ROS) level was measured by 2′7′-dichlorofluorescein diacetate intensity. Yuzu and HSP significantly ameliorated the increase in BBB permeability and the disruption of claudin-5 and ZO-1 in both in vivo and in vitro models. In bEnd.3 cells, yuzu and HSP were shown to inhibit the disruption of claudin-5 and ZO-1 during hypoxia, and the protective effects of yuzu and HSP on claudin-5 degradation seemed to be mediated by Forkhead box O 3a (FoxO3a) and matrix metalloproteinase (MMP)-3/9. In addition, well-known antioxidants, trolox and N-acetyl cysteine, significantly attenuated the BBB permeability increase, disruption of claudin-5 and ZO-1, and FoxO3a activation during hypoxia, suggesting that ROS are important mediators of BBB dysfunction during hypoxia. Collectively, these results indicate that yuzu and HSP protect the BBB against dysfunction via maintaining integrity of claudin-5 and ZO-1, and these effects of yuzu and HSP appear to be a facet of their antioxidant properties. Our findings may contribute to therapeutic strategies for BBB-associated neurodegenerative diseases.

Interactions between antiparkinsonian drugs and ABCB1/P-glycoprotein at the blood–brain barrier in a rat brain endothelial cell model

2008 ◽  
Vol 442 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Sarah Vautier ◽  
Aline Milane ◽  
Christine Fernandez ◽  
Marion Buyse ◽  
Helene Chacun ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Rat Brain ◽  
Blood Brain Barrier ◽  
Cell Model ◽  
Brain Barrier ◽  
Brain Endothelial Cell ◽  
P Glycoprotein ◽  
Blood Brain ◽  
Antiparkinsonian Drugs

scholarly journals The effect of nanoparticle size on the probability to cross the blood-brain barrier: an in-vitro endothelial cell model

2015 ◽  
Vol 13 (1) ◽  
pp. 19 ◽  
Author(s):  
Malka Shilo ◽  
Anat Sharon ◽  
Koby Baranes ◽  
Menachem Motiei ◽  
Jean-Paul M Lellouche ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Cell Model ◽  
Brain Barrier ◽  
Nanoparticle Size ◽  
Blood Brain
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