Ovarian tissue remodeling: role of matrix metalloproteinases and their inhibitors

2002 ◽  
Vol 191 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Michael F. Smith ◽  
William A. Ricke ◽  
Leanne J. Bakke ◽  
Mark P.D. Dow ◽  
George W. Smith
Keyword(s):  
Matrix Metalloproteinases ◽  
Ovarian Tissue ◽  
Tissue Remodeling

scholarly journals The role of matrix metalloproteinases in aging: Tissue remodeling and beyond

2017 ◽  
Vol 1864 (11) ◽  
pp. 2015-2025 ◽  
Author(s):  
Sandra Freitas-Rodríguez ◽  
Alicia R. Folgueras ◽  
Carlos López-Otín
Keyword(s):  
Matrix Metalloproteinases ◽  
Tissue Remodeling

Levels of MMP2, TIMP1 and TIMP2 in Follicular Fluids in Women Undergoing in Vitro Fertilization and Their Relationship to Oocyte Quality

2019 ◽  
Vol 12 (2) ◽  
pp. 100-107
Author(s):  
Nina P. Ayvazova ◽  
Lyubomira O. Ilieva ◽  
Emiliana I. Konova ◽  
Milena A. Atanasova
Keyword(s):  
Matrix Metalloproteinases ◽  
Ovarian Tissue ◽  
Follicular Development ◽  
Germinal Vesicle ◽  
Enzyme Linked Immunosorbent Assay ◽  
Oocyte Maturity ◽  
Vitro Fertilization ◽  
Follicular Fluids

Summary Recently, the important role of matrix metalloproteinases (MMPs) has been identified in follicular development and subsequent ovulation. Although the role of MMP in ovarian tissue remodeling during folliculogenesis has been well studied, the relationship between matrix protease activity and their inhibitors - Tisue inhibitors of matrix metalloproteinases (TIMP) and aging of the oocytes is still unclear. The present study aimed to establish the probable relationship between the expression levels of MMP-2 and TMP-1 and TIMP-2 in follicular fluid with the degree of oocyte maturity and quality. Follicular fluids from 20 women collected on the day of follicular puncture were tested for the presence of MMP-2, TIMP-1, and TIMP-2 using enzyme-linked immunosorbent assay (ELISA). The oocytes obtained were described in terms of maturity, morphology, and fertilization, as well as the embryo’s quality and rate of development. MMP-2 was significantly higher in follicular aspirates in the first prophase of meiosis - germinal vesicle (GV), compared to aspirates with first metaphase (MI) (p=0.011) and second metaphase (MII) of mature oocytes (p=0.010). The MMP-2/TIMP-1 ratio was significantly higher for GV compared to M1 (p=0.011), M2 (p=0.006) and atretic oocytes (p=0.032); (F(3, 71)=2.909, p=0.040). Based on our results, we can conclude that MMP-2 concentration in follicular fluids during the IVF / ICSI procedure had a significant relationship to oocyte maturation levels. It was significantly higher in the case of immature oocytes. On the other hand, oocytes with normal morphology were associated with a significantly higher MMP-2 concentration in follicular fluids.

scholarly journals A Tale of Two Joints: The Role of Matrix Metalloproteases in Cartilage Biology

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Brandon J. Rose ◽  
David L. Kooyman
Keyword(s):  
Rheumatoid Arthritis ◽  
Extracellular Matrix ◽  
Wound Healing ◽  
Matrix Metalloproteinases ◽  
Embryonic Development ◽  
Tissue Remodeling ◽  
Matrix Metalloproteases ◽  
Extracellular Matrix Molecules ◽  
Cartilage Biology

Matrix metalloproteinases are a class of enzymes involved in the degradation of extracellular matrix molecules. While these molecules are exceptionally effective mediators of physiological tissue remodeling, as occurs in wound healing and during embryonic development, pathological upregulation has been implicated in many disease processes. As effectors and indicators of pathological states, matrix metalloproteinases are excellent candidates in the diagnosis and assessment of these diseases. The purpose of this review is to discuss matrix metalloproteinases as they pertain to cartilage health, both under physiological circumstances and in the instances of osteoarthritis and rheumatoid arthritis, and to discuss their utility as biomarkers in instances of the latter.

ANDROST-5-ENE-3β,17β-DIOL IN OVARY OF POST-MENOPAUSAL HYPEROESTROGENIC WOMEN

1968 ◽  
Vol 58 (3) ◽  
pp. 364-376 ◽  
Author(s):  
S. Pesonen ◽  
M. Ikonen ◽  
B-J. Procopé ◽  
A. Saure
Keyword(s):  
Ovarian Tissue ◽  
Cortical Layer ◽  
Vaginal Smears ◽  
Incubation Experiments ◽  
Cell Groups ◽  
Post Menopause ◽  
Reducing Activity ◽  
Post Menopausal ◽  
One Year

ABSTRACT The ovaries of ten patients, at least one year after the post-menopause, were incubated with two Δ5-C19-steroids and also studied histochemically. All these patients had post-menopausal uterine bleeding and increased oestrogen excretion of the urine. The urinary estimations of gonadotrophins, 17-KS, 17-OHCS and pregnanediol were carried out on all patients. Vaginal smears were read according to Papanicolaou, and the endometrium and ovaries were studied histologically. The incubation experiments indicate the presence of Δ5-3β-hydroxysteroid-dehydrogenase. When androst-5-ene-3β,17β-diol was used as precursor the formation of testosterone occurred without any concomitant production of DHA and/or androstenedione. This seems to indicate the possible role of the Δ5-pathway in the formation of testosterone by post-menopausal ovarian tissue. The histochemical reactions indicated a reducing activity on NADH, lactate and glucose-6-phosphate, in certain corpora albicantia, atretic follicles and in diffuse thecoma regions in the cortical layer of the ovary. Steroid-3β-ol-dehydrogenase and β-hydroxybutyrate-dehydrogenase were found only at the edges of certain corpora albicantia, in some individual stroma cell groups and in some atretic follicles. Our studies, both biochemical and histochemical, suggest that the observed increase in the urinary oestrogens of the patients studied might in part at least, be of ovarian origin. This opinion is also supported by the postoperative oestrogen values.

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