Identification and characterization of the gene encoding human cytoplasmic polyadenylation element binding protein

Gene ◽  
2001 ◽  
Vol 263 (1-2) ◽  
pp. 113-120 ◽  
Author(s):  
Joseph F Welk ◽  
Amanda Charlesworth ◽  
Gary D Smith ◽  
Angus M MacNicol
Keyword(s):  
Binding Protein ◽  
Gene Encoding ◽  
Cytoplasmic Polyadenylation ◽  
Element Binding Protein ◽  
Identification And Characterization

scholarly journals Identification and Characterization of a Linear-Plasmid-Encoded Factor H-Binding Protein (FhbA) of the Relapsing Fever Spirochete Borrelia hermsii

2004 ◽  
Vol 186 (9) ◽  
pp. 2612-2618 ◽  
Author(s):  
Kelley M. Hovis ◽  
John V. McDowell ◽  
LaToya Griffin ◽  
Richard T. Marconi
Keyword(s):  
Molecular Mechanisms ◽  
Binding Protein ◽  
Genetic Locus ◽  
Factor H ◽  
Linear Plasmid ◽  
Relapsing Fever ◽  
Gene Encoding ◽  
Borrelia Hermsii ◽  
Identification And Characterization

ABSTRACT In North America, tick-borne relapsing fever (TBRF) is caused by the spirochete species Borrelia hermsii, Borrelia parkeri, and Borrelia turicatae. We previously demonstrated that some isolates of B. hermsii and B. parkeri are capable of binding factor H and that cell-bound factor H can participate in the factor I-mediated cleavage of C3b. Isolates that bound factor H expressed a factor H-binding protein (FHBP) that we estimated to be approximately 19 to 20 kDa in size and thus, pending further characterization, temporarily designated FHBP19. Until this report, none of the FHBPs of the TBRF spirochetes had been characterized. Here we have recovered the gene encoding the FHBP of B. hermsii YOR from a lambda ZAP II library and determined its sequence. The gene encodes a full-length protein of 22.7 kDa, which after processing is predicted to be 20.5 kDa. This protein, which we redesignate factor H-binding protein A (FhbA), is unique to B. hermsii. Two-dimensional pulsed-field gel electrophoresis and hybridization analyses revealed that the B. hermsii gene encoding FhbA is a single genetic locus that maps to a linear plasmid of approximately 220 kb. The general properties of FhbA were also assessed. The protein was found to be surface exposed and lipidated. Analysis of the antibody response to FhbA in infected mice revealed that it is antigenic during infection, indicating expression during infection. The identification and characterization of FhbA provides further insight into the molecular mechanisms of pathogenesis of the relapsing fever spirochetes.

Purification, Identification, and Characterization of an Osmotic Response Element Binding Protein

2000 ◽  
Vol 270 (1) ◽  
pp. 52-61 ◽  
Author(s):  
Ben C.B. Ko ◽  
Christoph W. Turck ◽  
Karen W.Y. Lee ◽  
Yinqing Yang ◽  
Stephen S.M. Chung
Keyword(s):  
Binding Protein ◽  
Response Element ◽  
Osmotic Response ◽  
Element Binding Protein ◽  
Identification And Characterization
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