103 High-throughput screening against intractable targets: the use of target disruption gene signatures for rapid cancer drug discovery

2004 ◽  
Vol 2 (8) ◽  
pp. 34
Author(s):  
P.E. Young ◽  
J. Castaneda ◽  
M. Chakiath ◽  
K. Evans ◽  
S.K. Horrigan ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cancer Drug ◽  
Gene Signatures ◽  
Cancer Drug Discovery

Abstract 4206: Utilizing a Drosophila high throughput screening technology for cancer drug discovery

2010 ◽  
Author(s):  
Elena M. Gracheva ◽  
Eduardo J. Martinez ◽  
Jill Thompson ◽  
Dominick Thompson ◽  
Ross L. Cagan
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cancer Drug ◽  
Cancer Drug Discovery ◽  
Screening Technology

scholarly journals Complex Tumor Spheroids, a Tissue-Mimicking Tumor Model, for Drug Discovery and Precision Medicine

2021 ◽  
pp. 247255522110383
Author(s):  
Gurmeet Kaur ◽  
David M. Evans ◽  
Beverly A. Teicher ◽  
Nathan P. Coussens
Keyword(s):  
Drug Discovery ◽  
Precision Medicine ◽  
Cell Lines ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cell Types ◽  
Response To Therapy ◽  
Cancer Drug ◽  
Malignant Cells ◽  
Pancreatic Cell

Malignant tumors are complex tissues composed of malignant cells, vascular cells, structural mesenchymal cells including pericytes and carcinoma-associated fibroblasts, infiltrating immune cells, and others, collectively called the tumor stroma. The number of stromal cells in a tumor is often much greater than the number of malignant cells. The physical associations among all these cell types are critical to tumor growth, survival, and response to therapy. Most cell-based screens for cancer drug discovery and precision medicine validation use malignant cells in isolation as monolayers, embedded in a matrix, or as spheroids in suspension. Medium- and high-throughput screening with multiple cell lines requires a scalable, reproducible, robust cell-based assay. Complex spheroids include malignant cells and two normal cell types, human umbilical vein endothelial cells and highly plastic mesenchymal stem cells, which rapidly adapt to the malignant cell microenvironment. The patient-derived pancreatic adenocarcinoma cell line, K24384-001-R, was used to explore complex spheroid structure and response to anticancer agents in a 96-well format. We describe the development of the complex spheroid assay as well as the growth and structure of complex spheroids over time. Subsequently, we demonstrate successful assay miniaturization to a 384-well format and robust performance in a high-throughput screen. Implementation of the complex spheroid assay was further demonstrated with 10 well-established pancreatic cell lines. By incorporating both human stromal and tumor components, complex spheroids might provide an improved model for tumor response in vivo.

Abstract 2171: Beta-hairpin peptide hydrogels as scaffolds for 3D high-throughput cancer drug discovery

2016 ◽  
Author(s):  
Peter Worthington ◽  
Katherine M. Drake ◽  
Andrew D. Napper ◽  
Darrin J. Pochan ◽  
Sigrid A. Langhans
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
Cancer Drug ◽  
Cancer Drug Discovery ◽  
Beta Hairpin ◽  
Peptide Hydrogels

scholarly journals Development of a High-Throughput Three-Dimensional Invasion Assay for Anti-Cancer Drug Discovery

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e82811 ◽  
Author(s):  
Nikki A. Evensen ◽  
Jian Li ◽  
Jie Yang ◽  
Xiaojun Yu ◽  
Nicole S. Sampson ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
Three Dimensional ◽  
Cancer Drug ◽  
Invasion Assay ◽  
Cancer Drug Discovery ◽  
Anti Cancer

scholarly journals A modified gene trap approach for improved high-throughput cancer drug discovery

Oncogene ◽  
2018 ◽  
Vol 37 (31) ◽  
pp. 4226-4238 ◽  
Author(s):  
Shelli M. Morris ◽  
Andrew J. Mhyre ◽  
Savanna S. Carmack ◽  
Carrie H. Myers ◽  
Connor Burns ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
Gene Trap ◽  
Cancer Drug ◽  
Cancer Drug Discovery
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