Comparison of supplemental cobalt form on fibre digestion and cobalamin concentrations in cattle

2017 ◽  
Vol 155 (5) ◽  
pp. 832-838 ◽  
Author(s):  
R. C. WATERMAN ◽  
W. L. KELLY ◽  
C. K. LARSON ◽  
M. K. PETERSEN

SUMMARYCobalt (Co) is essential for rumen microbial metabolism to synthesize methane, acetate and methionine. It also serves as a structural component of vitamin B12(cobalamin), which functions as a coenzyme in energy metabolism. A study was conducted to determine if Co form (carbonatev. glucoheptonate) supplemented above the National Research Council requirements would improve digestibility of a low-quality forage diet and change serum cobalamin concentrations. Nineteen ruminally cannulated cows (577 ± 13 kg) were fed individually in a completely randomized experimental design. Cows were fed a grass hay diet that contained (79·2 g/kg crude protein, 565 g/kg total digestible nutrients, 633·2 g/kg neutral detergent fibre (NDF), 874·2 g/kg dry matter) at a rate of 0·02% of body weight on a as fed basis for a 62-day study, which consisted of three periods; acclimation (AC), treatment (TR) and residual (RE). Measurements taken in the AC period were used as covariates for analysis in the TR and RE periods. Cows were stratified by age (5 ± 0·4 years) and lactational history, and assigned to receive 12·5 mg supplemental Co in one of two forms: (1) 27·2 mg of Co carbonate (CC,n= 11 cows) or (2) 50 mg of Co glucoheptonate (CGH,n= 8 cows). Supplement was administered daily via a gelatin capsule placed directly into the rumen 2 h after feeding. During the last 96 h of each period, forage digestibility was measured using anin situnylon bag technique. Blood samples were collected 4 and 6 h following feeding, and 24 h before the end of each period. A treatment × period interaction was detected forin situorganic matter (OM) disappearance at 96 h; (TR period: 684 and 708 ± 81 g/kg; RE period: 676 and 668 ± 75 g/kg, for CC and CGH, respectively). Once inclusion of Co in the CGH group was removed, OM disappearance was reduced by 4·01% compared with 0·82% in the CC cows. The NDF disappearance (OM basis) was less for the TR compared with the RE at 48 h (629 and 652 ± 39 g/kg, respectively). However, by 96 h NDF disappearance was greater for TR than the RE (704 and 689 ± 44 g/kg; respectively). No differences were detected for cobalamin serum concentrations or rate of fibre fermentation. The outcomes of the current research signify that there may be a slight residual effect of Co supplementation on fermentation; there was also an indication that Co source may enhance the overall extent of fermentation.

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Oswaldo Rosendo ◽  
Luis Freitez ◽  
Rafael López

In in vitro true dry matter degradability (IVTDMD), in situ dry matter degradability, and neutral detergent fiber degradability, both in vitro (IVNDFD) and in situ (ISNDFD) techniques were used with crossbred goats to determine dry matter and neutral detergent fiber (NDF) ruminal degradability in eight forages and four industrial byproducts. Total digestible nutrients (TDN) content obtained with five different summative models (summative equations) were studied to compare the precision of estimates. All these models included digestible fractions of crude protein, ether extract, and nonfiber carbohydrates that were calculated from chemical composition, but digestible NDF (dNDF) was obtained from IVNDFD (IVdNDF), ISNDFD (ISdNDF), or by using the Surface Law approach. On the basis of the coefficient of determination (R2) of the simple lineal regression of predicted TDN (y-axes) and observed IVTDMD (x-axes), the precision of models was tested. The predicted TDN by the National Research Council model exclusively based on chemical composition only explains up to 41% of observed IVTDMD values, whereas the model based on IVdNDF had a high precision (96%) to predict TDN from forage and byproducts fiber when used in goats.


Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.


2017 ◽  
Vol 9 (3) ◽  
pp. 226
Author(s):  
Dheynne Alves Vieira ◽  
Andréia Santos Cezário ◽  
Wallacy Barbacena Rosa dos Santos ◽  
Jeferson Corrêa Ribeiro ◽  
Tiago Neves Pereira Valente ◽  
...  

The aim of this study was to evaluate the performance of crossbred Holstein × Zebu steers fed on the diets containing sugarcane in natura or ensiled with two levels of concentrate (30% and 70%). A total of 32 males of 394 kg of body weight were used in a completely randomized experimental design with four treatments and seven repetitions. The animals reported a high dry matter intake (DMI) (P < 0.05) when fed on the high concentrate levels. The increase in concentrate levels resulted in an approximately 25% to 60% higher DMI as compared to the lowest level offered by silage-based diets and sugarcane in natura. The highest apparent digestibility coefficients of dry matter were observed in the diets based on sugarcane in natura (P < 0.05). The sugarcane based diets also affected (P > 0.05) the digestibility of organic matter, neutral detergent fiber, crude protein, ether extract, and non-fiber carbohydrates. There were no differences (P > 0.05) between the diets for average daily weight gain, carcass dressing, carcass gain, and feed conversion. The diets based on sugarcane in nature or ensiled with 30% and 70% concentrates do not influence the performance of crossbred Holstein × Zebu cattle.


Author(s):  
Ivone Rodrigues da Silva ◽  
Francirose Shigaki ◽  
Rosane Cláudia Rodrigues ◽  
Ana Paula Ribeiro Jesus ◽  
Clésio dos Santos Costa ◽  
...  

ABSTRACT The objective of this study was to evaluate the nutritive value of sugarcane silage with or without inoculation with P. acidipropionici or L. buchneri, over three fermentation periods. The experimental design was completely randomized in a 3 x 3 inoculant by fermentation period factorial arrangement (without inoculant, inoculant 1, inoculant 2; x three fermentation periods, 10, 60, 90 days). Values of pH, dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM) and lignin were determined and in situ DM degradability profiles were modelled for parameters a, b and c, potential degradation (A) and effective degradability (ED). The 90 day fermentation yielded a lower pH for both inoculants. There was an interaction between inoculant and fermentation period (P < 0.05) for DM content, with a reduction in silage DM without the additive at 90 days. The CP, HEM, ADF and lignin contents of sugarcane were not influenced by the treatments. The addition of P. acidipropionici provided the lowest NDF content at 10 days and presented a higher fraction a, potential degradation and ED. At 60 days, there was no variation in soluble fraction, the control silage showed a higher fraction b, higher potential degradation and ED. At 90 days of fermentation, L. buchneri silages presented a higher fraction a, degradation rate and DE and a higher b value was obtained in the silage without inoculant. Inoculants are effective in maintaining the silage DM content and nutritional value during prolonged fermentation periods.


2020 ◽  
Vol 100 (1) ◽  
pp. 165-174 ◽  
Author(s):  
Piotr Micek ◽  
Katarzyna Słota ◽  
Paweł Górka

The aim of this study was to determine the effect of heat treatment alone or in combination with the addition of lignosulfonate (LSO3) on canola cake protein, lysine, and methionine degradation in the rumen. Cold-pressed canola cake was left untreated, heated at 90, 110, 130, or 150 °C, or processed with 5% of LSO3 (in dry matter) and then heated. Effective rumen degradability of crude protein (CP), lysine, and methionine was less for treated than untreated canola cake (P < 0.05) and decreased with increased temperature of heating, but particularly when canola cake was heated at 150 °C (quadratic, P < 0.01). In general, effective rumen degradability of CP, lysine, and methionine was less for canola cake heated at 130 °C in combination with LSO3 compared with canola cake heat treated only (quadratic × LSO3 interaction, P ≤ 0.07). Results of this study indicate that high temperature heating (130 °C or greater for 60 min) may be necessary to protect canola cake protein from degradation in the rumen, and the combination of heat treatment and LSO3 may be more effective in protecting canola cake protein, lysine, and methionine from degradation in the rumen than the use of heat treatment only.


2000 ◽  
Vol 80 (1) ◽  
pp. 215-219 ◽  
Author(s):  
Marina A. G. von Keyserlingk ◽  
E. Weurding ◽  
Mary Lou Swift ◽  
C. F. Wright ◽  
J. A. Shelford ◽  
...  

The effect of adding 5% calcium lignosulfonate (LSO3) and heat to canola screenings on in situ ruminal and intestinal dry matter (DM) and crude protein (CP) disappearance in Holstein cows was investigated. Treatments consisted of untreated canola screenings, canola screenings that had been heated to 100 °C for 30 or 60 min and canola screenings that had 5% (wt wt−1) LSO3 added and were subsequently heated at 100 °C for 0, 30 or 60 min. Results indicate that treatment of canola screenings with 5% LSO3 and heated to 100 °C for 60 min was effective in reducing rumen degradation of DM and CP with a corresponding increase in disappearance in the lower gastrointestinal tract when compared to untreated and heat-treated canola screenings. Key words: Canola screenings, rumen by-pass protein, heat treatment, lignosulfonate


1999 ◽  
Vol 82 (9) ◽  
pp. 1978-1990 ◽  
Author(s):  
J.C. Elizalde ◽  
N.R. Merchen ◽  
D.B. Faulkner

2012 ◽  
Vol 10 (3) ◽  
pp. 285 ◽  
Author(s):  
Rafael Henrique De Tonissi e Buschinelli ◽  
Rita De Cássia Menchon Tramontini ◽  
Sérgio Tosi Cardim ◽  
Gabriel Daltoé de Almeida ◽  
Juliane Ribeiro ◽  
...  

O objetivo deste trabalho foi avaliar a degradabilidade ruminal da matéria seca e proteína bruta por meio da técnica in situ dos capins Brachiaria brizantha e Panicum maximum cortados aos 60 dias de idade, das silagens de milho, sorgo e milheto, dos fenos de coast cross, guandu e soja perene e do farelo de soja. Foram utilizados três novilhos com peso médio de 300 kg, fistulados e providos de cânulas ruminais. Os volumosos foram incubados em ordem decrescente de 72, 48, 24, 18, 12, 6, 3 e 0h, e o farelo de soja incubado nos tempos 48, 24, 18, 12, 6, 3 e 0h. Os resíduos dos sacos foram analisados quanto aos teores de matéria seca e proteína bruta. O farelo de soja apresentou alta degradabilidade efetiva para MS em função das frações solúveis (32,85%) e fração potencialmente degradável (65,75%). A silagem de sorgo apresentou elevada fração solúvel e potencialmente degradável se comparada às demais silagens. O feno de soja perene apresentou a maior degradabilidade de MS (80,42%), entre os volumosos avaliados. Para a proteína, destacam-se a baixa degradabilidade apresentada pelo feno de guandu (36,91%) e os demais volumosos apresentaram degradabilidade variando entre 64,50 a 74,56%.


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