Identification of maize mosaic virus by immunoelectron microscopy

1986 ◽  
Vol 44 ◽  
pp. 240-241
Author(s):  
O. E. Bradfute
Keyword(s):  
Zea Mays ◽  
Mosaic Virus ◽  
Immunoelectron Microscopy ◽  
Severe Disease ◽  
Maize Mosaic Virus ◽  
The World ◽  
Plant Rhabdovirus

Maize mosaic virus (MMV) causes a severe disease of Zea mays in many tropical and subtropical regions of the world, including the southern U.S. (1-3). Fig. 1 shows internal cross striations of helical nucleoprotein and bounding membrane with surface projections typical of many plant rhabdovirus particles including MMV (3). Immunoelectron microscopy (IEM) was investigated as a method for identifying MMV. Antiserum to MMV was supplied by Ramon Lastra (Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela).

Identification of Maize Rayado Fino Virus by Immunoelectron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 636-637
Author(s):  
O. E. Bradfute
Keyword(s):  
Electron Microscopy ◽  
Zea Mays ◽  
Costa Rica ◽  
Severe Disease ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Virus Particles ◽  
Far North ◽  
Maize Rayado Fino Virus ◽  
Antibody Coating

Maize rayado fino virus (MRFV) causes a severe disease of corn (Zea mays) in many locations throughout the neotropics and as far north as southern U.S. MRFV particles detected by direct electron microscopy of negatively stained sap from infected leaves are not necessarily distinguishable from many other small isometric viruses infecting plants (Fig. 1).Immunosorbent trapping of virus particles on antibody-coated grids and the antibody coating or decoration of trapped virus particles, was used to confirm the identification of MRFV. Antiserum to MRFV was supplied by R. Gamez (Centro de Investigacion en Biologia Celular y Molecular, Universidad de Costa Rica, Ciudad Universitaria, Costa Rica).Virus particles, appearing as a continuous lawn, were trapped on grids coated with MRFV antiserum (Fig. 2-4). In contrast, virus particles were infrequently found on grids not exposed to antiserum or grids coated with normal rabbit serum (similar to Fig. 1). In Fig. 3, the appearance of the virus particles (isometric morphology, 30 nm diameter, stain penetration of some particles, and morphological subunits in other particles) is characteristic of negatively stained MRFV particles. Decoration or coating of these particles with MRFV antiserum confirms their identification as MRFV (Fig. 4).

scholarly journals Molecular and Biological Characterisation of Turnip mosaic virus Isolates Infecting Poppy (Papaver somniferum and P. rhoeas) in Slovakia

Viruses ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 430 ◽  
Author(s):  
Miroslav Glasa ◽  
Katarína Šoltys ◽  
Lukáš Predajňa ◽  
Nina Sihelská ◽  
Slavomíra Nováková ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Turnip Mosaic Virus ◽  
Biological Assays ◽  
Genetic Complexity ◽  
The World ◽  
History Of ◽  
Virus Isolates

In recent years, the accumulated molecular data of Turnip mosaic virus (TuMV) isolates from various hosts originating from different parts of the world considerably helped to understand the genetic complexity and evolutionary history of the virus. In this work, four complete TuMV genomes (HC9, PK1, MS04, MS15) were characterised from naturally infected cultivated and wild-growing Papaver spp., hosts from which only very scarce data were available previously. Phylogenetic analyses showed the affiliation of Slovak Papaver isolates to the world-B and basal-B groups. The PK1 isolate showed a novel intra-lineage recombination pattern, further confirming the important role of recombination in the shaping of TuMV genetic diversity. Biological assays indicated that the intensity of symptoms in experimentally inoculated oilseed poppy are correlated to TuMV accumulation level in leaves. This is the first report of TuMV in poppy plants in Slovakia.

scholarly journals Estado del Cultivo de Plátano (Musa sp) en el Municipio de Tlapacoyan, Veracruz

UVserva ◽  
2018 ◽  
Author(s):  
Paloma Violeta Susan Tepetlan
Keyword(s):  
Triticum Aestivum ◽  
Zea Mays ◽  
Oryza Sativa ◽  
Banana Production ◽  
Tropical Countries ◽  
Musa Sp ◽  
Important Crop ◽  
The World

Los bananos y plátanos (Musa sp) se cultivan en más de 120 países alrededor del mundo y suministran una importante fuente de carbohidratos para más de 400 millones de personas en países tropicales, representan el cuarto cultivo de mayor importancia después del maíz (Zea mays), trigo (Triticum aestivum) y arroz (Oryza sativa). En México, se cultivan en 15 Estados, la variedad de bananos y plátanos que se cultivan  es amplia, dentro de ellas destacan los bananos como el Plátano Tabasco o Roatán, Enano Gigante, Criollo, Valery, Dominico, Manzano y los plátanos Macho, Morado y Pera, entre otros. Veracruz, ocupa el tercer lugar a nivel nacional en su, después de Chiapas y Tabasco, produciéndose en zonas en las que el cultivo es una de las principales actividades.Palabras clave: cultivo de plátano; Veracruz; países tropicales; Tlapacoyan Abstract Bananas and plantains are grown in more than 120 countries around the world, provides an important source of carbohydrates for more than 400 million people in tropical countries, is the fourth most important crop after maize (Zea mays), wheat (Triticum aestivum) and rice (Oryza sativa). In Mexico, are grown in 15 states, banana and plantain cultivars that are grown is wide, within them we can found bananas as Tabasco or Roatán, Giant Dwarf, Macho, Criollo, Valery, Dominico, Manzano and plantains as Morado and Pera between others. Veracruz, is third in banana production, after Chiapas and Tabasco, taking place in areas where this crop is one of the main activities.Keywords: banana cultivation; Veracruz; tropical countries; Tlapacoyan

scholarly journals Leptospirosis

2016 ◽  
Vol 8 (3) ◽  
Author(s):  
Novie H. Rampengan
Keyword(s):  
Gold Standard ◽  
Case Fatality Rate ◽  
Severe Disease ◽  
Case Fatality ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Moderate Risk ◽  
Rat Urine ◽  
The World ◽  
Different Parts

Abstract: Leptospirosis is a zoonotic disease that usually occurs during the flood and is generally transmitted through rat urine. Indonesia is a country with a moderate risk of transmission of leptospirosis. Leptospirosis has a broad manifestation varying from self-limited to severe disease. The gold standard examination of leptospirosis is microscopic agglutination test. Diagnosis is divided into suspected, probable, and confirmed. Treatment consists of antibiotics and supportive agents. Generally, the prognosis is good, albeit, sequelae can occur. Case-fatality rate in different parts of the world ranging from less than 5% to 30%.Keywords: leptospirosis, diagnosis,Abstrak: Leptospirosis merupakan penyakit zoonosis yang umumnya timbul saat banjir dan umumnya ditularkan melalui kencing tikus. Indonesia merupakan negara dengan risiko sedang penularan leptospirosis. Leptospirosis memiliki manifestasi luas dari self limited hingga sakit berat. Pemeriksaan baku emas leptospirosis ialah dengan microscopic agglutination test. Diagnosis dibagi atas suspek, probable, dan konfirmasi. Terapi diberikan medikamentosa dengan antibiotik dan suportif. Prognosis umumnya baik namun bisa juga terjadi gejala sisa. Tingkat fatalitas kasus di berbagai belahan dunia berkisar <5%-30%.Kata kunci: leptospirosis, diagnosis

Subcellular localization and detection of Tobacco mosaic virus ORF6 protein by immunoelectron microscopy

2017 ◽  
Vol 82 (1) ◽  
pp. 60-66 ◽  
Author(s):  
T. N. Erokhina ◽  
E. A. Lazareva ◽  
K. R. Richert-Pöggeler ◽  
E. V. Sheval ◽  
A. G. Solovyev ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Immunoelectron Microscopy ◽  
Orf6 Protein

Genome-Wide Analysis of Alternative Splicing in Zea mays during Maize Iranian Mosaic Virus Infection

2019 ◽  
Vol 37 (5-6) ◽  
pp. 413-420 ◽  
Author(s):  
Abozar Ghorbani ◽  
Ahmad Tahmasebi ◽  
Keramatollah Izadpanah ◽  
Alireza Afsharifar ◽  
Ralf G. Dietzgen
Keyword(s):  
Zea Mays ◽  
Alternative Splicing ◽  
Virus Infection ◽  
Mosaic Virus ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Mosaic Virus Infection

Molecular mapping of a major gene conferring resistance to maize mosaic virus

1997 ◽  
Vol 95 (1-2) ◽  
pp. 271-275 ◽  
Author(s):  
R. Ming ◽  
J. L. Brewbaker ◽  
R. C. Pratt ◽  
T. A. Musket ◽  
M. D. McMullen
Keyword(s):  
Mosaic Virus ◽  
Molecular Mapping ◽  
Major Gene ◽  
Maize Mosaic Virus

scholarly journals The occurrence of maize mosaic virus on sorghum in India

1989 ◽  
Vol 114 (2) ◽  
pp. 301-310 ◽  
Author(s):  
R. A. NAIDU ◽  
R. HARIKRISHNAN ◽  
S. K. MANOHAR ◽  
D. V. R. REDDY ◽  
A. S. RATNA ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Maize Mosaic Virus
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