scholarly journals Taking Two-Photon Excitation (2PE) further: 2PE coupling to Far-field Optical Nanoscopy and Super Resolution Microscopy towards Three-dimensional (3D) Imaging of Thick Scattering Specimens

2013 ◽  
Vol 19 (S2) ◽  
pp. 166-167
Author(s):  
A. Diaspro ◽  
P. Bianchini ◽  
F. Cella Zanacchi ◽  
G. Vicidomini ◽  
B. Harke ◽  
...  
Keyword(s):  
3D Imaging ◽  
Three Dimensional ◽  
Super Resolution ◽  
Far Field ◽  
Two Photon ◽  
Photon Excitation ◽  
Super Resolution Microscopy ◽  
Two Photon Excitation

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

Two-Photon Excitation Imaging of Glucose Metabolism in Living Tissue

1997 ◽  
Vol 3 (S2) ◽  
pp. 305-306
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Collection Efficiency ◽  
Three Dimensional ◽  
Spatial Filter ◽  
Input Power ◽  
Photon Absorption ◽  
Focal Volume ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. It provides three-dimensional resolution and eliminates background equivalent to an ideal confocal microscope without requiring a confocal spatial filter, whose absence enhances fluorescence collection efficiency. This results in inherent submicron optical sectioning by excitation alone. In practice, TPEM is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 limits the average input power to less than 10 mW, only slightly greater than the power normally used in confocal microscopy. Because of the intensity-squared dependence of the two-photon absorption, the excitation is limited to the focal volume.

Crosstalk in Photoluminescence Readout of Three-Dimensional Memory in Vitreous Silica by One- and Two-Photon Excitation

2000 ◽  
Vol 39 (Part 1, No. 12A) ◽  
pp. 6763-6767 ◽  
Author(s):  
Mitsuru Watanabe ◽  
Saulius Juodkazis ◽  
Shigeki Matsuo ◽  
Junji Nishii ◽  
Hiroaki Misawa
Keyword(s):  
Three Dimensional ◽  
Vitreous Silica ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation
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