Inheritance of evolved thiocarbamate resistance in Rigid Ryegrass (Lolium rigidum) populations from Australia

Weed Science ◽  
2021 ◽  
pp. 1-19
Author(s):  
David J. Brunton ◽  
Peter Boutsalis ◽  
Gurjeet Gill ◽  
Christopher Preston

Abstract Populations of rigid ryegrass (Lolium rigidum Gaudin) from southern Australia have evolved resistance to the thiocarbamate herbicide prosulfocarb. The inheritance of prosulfocarb resistance was explored by crossing R and S individuals. In all families within each cross, except 16.2, the response of the F1 were intermediate between the parents, suggesting that resistance is inherited as a single, partially dominant trait. For 16.2, the response of the F1 was more similar to the susceptible parent, suggesting resistance may be a recessive trait in this population. Segregation at the discriminating dose of 1200 g a.i. ha−1 prosulfocarb in populations 375-14 fitted the ratio (15:1) consistent with two independent dominant alleles; 198-15 fitted a ratio (13:3) for two independent alleles, one dominant and one recessive; and EP162 fitted a ratio (9:7) for two additive dominant alleles. In contrast segregation of population 16.2 fitted a (7:9) ratio consistent with two independent recessive alleles contributing to prosulfocarb resistance. Four different patterns of resistance to prosulfocarb were identified in different resistant populations, with inheritance as a dominant allele, dominant and recessive, additive dominant and as an independent recessive allele. This suggests there are several different mechanisms of prosulfocarb resistance present in L. rigidum.

Weed Science ◽  
2005 ◽  
Vol 53 (5) ◽  
pp. 615-619 ◽  
Author(s):  
Marulak Simarmata ◽  
Suleiman Bughrara ◽  
Donald Penner

Glyphosate resistance was found in a rigid ryegrass population in northern California. A sample of the resistant plants were collected and grown under greenhouse conditions. The objective of this study was to evaluate glyphosate resistance in the progeny of the collected plants by recurrent selection, obtain the homozygous resistant and sensitive lines to establish dose-response curves, and to determine the inheritance of glyphosate resistance in rigid ryegrass. Diverse levels of resistance were observed in the first generation with survival of 89, 59, 45, and 9% from glyphosate at 1x, 2x, 4x, and 8x respectively, where x = 1.12 kg ha−1isopropylamine salt of glyphosate. Clones of plants that died from 1x were allowed to produce seed and were further subjected to recurrent selection to generate the most sensitive plants (S lines), which died from 0.125x glyphosate. The most resistant plants (R lines) were generated from the survivors receiving 8x glyphosate. The ratio between I50rates for the glyphosate resistant and the glyphosate sensitive plants was > 100-fold. The R and S lines were crossed reciprocally and F1progeny of both (R × S) and (S × R) showed intermediate resistance. These survived up to 2x glyphosate. The F2progeny were generated by intercrossing of F1plants. The ratio of sensitive, intermediate, and resistant plants in the F2population before the treatment of glyphosate at 0.125x followed by 8x was 1 : 16, 14 : 16, and 1 : 16 respectively, which corresponded to the Mendelian segregation ratio of two genes. The results indicated that the inheritance of glyphosate resistance in rigid ryegrass from California appeared to be nuclear, incompletely dominant, multigenic, and pollen-transmitted with no indication of maternal inheritance.


Weed Science ◽  
2016 ◽  
Vol 64 (2) ◽  
pp. 207-215 ◽  
Author(s):  
Rupinder Kaur Saini ◽  
Jenna Malone ◽  
Christopher Preston ◽  
Gurjeet S. Gill

Rigid ryegrass, an important annual weed species in cropping regions of southern Australia, has evolved resistance to 11 major groups of herbicides. Dose–response studies were conducted to determine response of three clethodim-resistant populations and one clethodim-susceptible population of rigid ryegrass to three different frost treatments (−2 C). Clethodim-resistant and -susceptible plants were exposed to frost in a frost chamber from 4:00 P.M. to 8:00 A.M. for three nights before or after clethodim application and were compared with plants not exposed to frost. A reduction in the level of clethodim efficacy was observed in resistant populations when plants were exposed to frost for three nights before or after clethodim application. In the highly resistant populations, the survival percentage and LD50were higher when plants were exposed to frost before clethodim application compared with frost after clethodim application. However, frost treatment did not influence clethodim efficacy of the susceptible population. Sequencing of the acetyl coenzyme A carboxylase (ACCase) gene of the three resistant populations identified three known mutations at positions 1781, 2041, and 2078. However, most individuals in the highly resistant populations did not contain any known mutation in ACCase, suggesting the resistance mechanism was a nontarget site. The effect of frost on clethodim efficacy in resistant plants may be an outcome of the interaction between frost and the clethodim resistance mechanism(s) present.


2020 ◽  
Vol 17 ◽  
pp. 00070
Author(s):  
G. E. Osipov ◽  
Z. A. Osipova

The aim of the research was to establish the features of the inheritance of the size of the fruit in the hybrid families of Prunus domestica. The objects of study were hybrid seedlings of plum selection of the Tatar research Institute of agriculture. Fruit sizes were estimated according to the methodology of the all-Russian research Institute of fruit crops selection. The analysis of the splitting of plum in hybrid families by the size of fruits showed that seedlings with small fruits dominated in the breeding gardens of the Tatar research Institute. The size of the plum fruit is controlled by polygens. All of the original parental forms are heterozygotes for the genes determining the size of the fruit. A small fruit is a dominant trait, a large fruit is a recessive trait. In most hybrid families, plum seedlings have a significant variability in the size of the fruit. Transgressive genotypes with large fruits are formed in hybrid families Eurasia 21 x Renklod Tenkovsky, Eurasia 21 x free pollination and Zyuzinskaya x free pollination. The varieties Eurasia 21 and Zyuzinskaya must be used as sources in breeding of plums for large-fruited.


Author(s):  
Ioannis ROUSSIS ◽  
Ilias TRAVLOS ◽  
Ioanna KAKABOUKI ◽  
Spyridoula CHAVALINA ◽  
Varvara KOUNELI ◽  
...  

A pot experiment was conducted to evaluate the allelopathic potential of Nigella sativa L. on seedling emergence and first growth of the serious weed rigid ryegrass (Lolium rigidum L.). The results revealed significant weed suppressive effects of N. sativa residues incorporated into the soil. The lowest seedling emergence rate (38%) was observed in pots treated with 20 g leaf residues. Regarding height, the lowest value (26.7 cm) was found in 20 g root residues. The highest quantity of shoot residues reduced leaf number, tiller number, fresh and dry aerial biomass up to 58, 51, 64 and 59%, respectively. It can be concluded that the effect of incorporation of plant residues depended on the type and amount of residue. N. sativa could be utilized as an allelopathic crop to control weeds and future studies should focus on isolating and identifying allelochemicals in roots, shoots and leaves of this species.


Weed Science ◽  
2012 ◽  
Vol 60 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Sudheesh Manalil ◽  
Roberto Busi ◽  
Michael Renton ◽  
Stephen B. Powles

A wild population of a plant species, especially a cross-pollinated species, can display considerable genetic variation. Genetic variability is evident in differential susceptibility to an herbicide because the population can show continuous phenotypic variation. Recent, recurrent selection studies have revealed that phenotypic variation in response to low herbicide rates is heritable and can result in rapid evolution of herbicide resistance in genetically variable cross-pollinated rigid ryegrass. In this study, the heritable genetic variation in an herbicide-susceptible rigid ryegrass population was exploited to shift the population toward greater herbicide susceptibility by recurrent selection. To enhance herbicide susceptibility, herbicide-susceptible rigid ryegrass plants were divided into two identical clones, and one series of cloned plants was treated with a low rate of herbicide (diclofop). The nontreated clones of individuals that did not survive the herbicide treatment were selected and bulk-crossed to obtain the susceptible progeny. After two cycles of selection, the overall susceptibility to diclofop was doubled. The results indicate that minor genes for resistance are present in an herbicide-susceptible rigid ryegrass population, and their exclusion can increase susceptibility to diclofop.


1982 ◽  
Vol 2 (2) ◽  
pp. 97-105 ◽  
Author(s):  
N Bouck ◽  
G di Mayorca

Treatment of BHK cells with mutagenic carcinogens induced neoplastic transformation in a single step. This transformation displayed the characteristics expected for a recessive mutation. Increasing doses of carcinogens induced transformants with kinetics similar to the kinetics with which they induced 6-thioguanine-resistant or ouabain-resistant mutants in the same population of cells. Transformants with temperature-restricted phenotypes were easily induced by carcinogens which cause mutations by base changes, but when ICR frameshift mutagens were used, the proportion of temperature-limited transformants was inversely related to the frequency with which a particular mutagen induced frameshift mutations. In hybrids between pseudodiploid isogenic strains of normal and transformed BHK cells, transformation was expressed as a dominant trait when the transformed parent was induced by a papovavirus, but was suppressed as a recessive trait when the transformed parent arose spontaneously or was chemically induced. Segregation of transformation was observed upon growth of suppressed normal hybrids, and the transformed phenotype which was reexpressed was in most cases characteristics of the original transformed parent.


2014 ◽  
Vol 36 (4) ◽  
pp. 1051-1051
Author(s):  
Diana Saja ◽  
Magdalena Rys ◽  
Agnieszka Stokłosa ◽  
Andrzej Skoczowski

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