NIR-II Ratiometric Lanthanide-Dye Hybrid Nanoprobes Doped Bioscaffolds for In Situ Bone Repair Monitoring

The present work focuses on the preparation of poly(l–lactide)–magnesium oxide whiskers (PLLA–MgO) composites by the in-situ polymerization method for bone repair and implant. PLLA–MgO composites were evaluated using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and solid-state 13C and 1H nuclear magnetic resonance spectroscopy (NMR). It was found that the whiskers were uniformly dispersed in the PLLA matrix through the interfacial interaction bonding between PLLA and MgO; thereby, the MgO whisker was found to be well-distributed in the PLLA matrix, and biocomposites with excellent interface bonding were produced. Notably, the MgO whisker has an effect on the crystallization behavior and mechanical properties; moreover, the in vivo degradation of PLLA–MgO composites could also be adjusted by MgO. These results show that the whisker content of 0.5 wt % and 1.0 wt % exhibited a prominent nucleation effect for the PLLA matrix, and specifically 1.0 wt % MgO was found to benefit the enhanced mechanical properties greatly. In addition, the improvement of the degrading process of the composite illustrated that the MgO whisker can effectively regulate the degradation of the PLLA matrix as well as raise its bioactivity. Hence, these results demonstrated the promising application of PLLA–MgO composite to serve as a biomedical material for bone-related repair.

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Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture model

Journal of Orthopaedic Surgery and Research ◽

10.1186/s13018-020-01953-7 ◽

2020 ◽

Vol 15 (1) ◽

Author(s):

Shintaro Shoji ◽

Kentaro Uchida ◽

Wataru Satio ◽

Hiroyuki Sekiguchi ◽

Gen Inoue ◽

...

Keyword(s):

Bone Morphogenetic Protein ◽

Bone Repair ◽

Fracture Site ◽

Bone Union ◽

Fracture Model ◽

Bone Morphogenetic Protein 2 ◽

Morphogenetic Protein ◽

And Control ◽

Gene Expression Levels

Abstract Background An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHs). Several studies have reported the application of IFHs in cell delivery and tissue engineering. IFHs may also be ideal carrier materials for bone repair, although their potential as a carrier for bone morphogenetic protein (BMP)-2 has yet to be examined. Here, we examined the effect of an IFH made of hyaluronic acid (IFH-HA) containing BMP-2 in promoting osteogenesis in a mouse refractory fracture model. Methods Immediately following a fracture procedure, animals either received no treatment (control) or an injection of IFH-HA/PBS or IFH-HA containing 2 μg BMP-2 (IFH-HA/BMP-2) into the fracture site (n = 16, each treatment). Results Fracture sites injected with IFH-HA/BMP-2 showed significantly greater bone volume, bone mineral content, and bone union compared with sites receiving no treatment or treated with IFH-HA/PBS alone (each n = 10). Gene expression levels of osteogenic markers, Alpl, Bglap, and Osx, were significantly raised in the IFH-HA/BMP-2 group compared to the IFH-HA/PBS and control groups (each n = 6). Conclusion IFH-HA/BMP-2 may contribute to the treatment of refractory fractures.

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Thermoresponsive hydrogels based on a phosphorylated star-shaped copolymer: mimicking the extracellular matrix for in situ bone repair

Journal of Materials Chemistry B ◽

10.1039/c6tb02657e ◽

2017 ◽

Vol 5 (3) ◽

pp. 428-434 ◽

Cited By ~ 14

Author(s):

Wei Wu ◽

Zaifu Lin ◽

Yanpeng Liu ◽

Xinyuan Xu ◽

Chunmei Ding ◽

...

Keyword(s):

Extracellular Matrix ◽

Cell Proliferation ◽

Bone Repair ◽

Star Polymer ◽

Thermoresponsive Hydrogels ◽

Gel Transition

A bioinspired hydrogel prepared using a star-polymer exhibits sol to gel transition to induce in situ biomineralization and facilitate cell proliferation.

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Calcium and phosphorus co-doped carbon dots enhance osteogenic differentiation for calvarial defect repair in situ

10.21203/rs.3.rs-698683/v1 ◽

2021 ◽

Author(s):

Lin Wu ◽

Yunchao Wu ◽

Jingjin Liu ◽

Suwan Liu ◽

Qianzhe Li ◽

...

Keyword(s):

Bone Repair ◽

Emission Spectra ◽

Treatment Method ◽

Temperature Sensitive ◽

Calvarial Defect ◽

Calvarial Bone ◽

Bone Injury ◽

Calcium And Phosphorus ◽

Doped Carbon Dots

Abstract Calvarial bone defect remains a clinical challenge due to the lack of efficient osteo-inductive agent. Herein, a novel calcium and phosphorus codoped carbon dot (Ca/P-CD) for bone regeneration was synthesized using phosphoethanolamine and calcium gluconate as precursors. The resultant Ca/P-CDs exhibited ultra-small size, stable excitation dependent emission spectra and favorable dispersibility in water. Moreover, Ca/P-CDs with good biocompatibility rapidly entered the cytoplasm through endocytosis and increased the expression of bone differentiation genes. After mixing with temperature-sensitive hydrogel, Ca/P-CDs were injected in situ into calvarial defect and promoted the repair of bone injury. These Ca/P-CDs provide a new treatment method for the bone repair and expend the application in the biomedical fields.

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Development of a Bone-Mimetic 3D Printed Ti6Al4V Scaffold to Enhance Osteoblast-Derived Extracellular Vesicles’ Therapeutic Efficacy for Bone Regeneration

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.757220 ◽

2021 ◽

Vol 9 ◽

Author(s):

Kenny Man ◽

Mathieu Y. Brunet ◽

Sophie Louth ◽

Thomas E. Robinson ◽

Maria Fernandez-Rhodes ◽

...

Keyword(s):

Bone Regeneration ◽

Extracellular Vesicles ◽

Therapeutic Efficacy ◽

Bone Marrow Stromal Cells ◽

Bone Repair ◽

Calcium Deposition ◽

Tissue Culture Plastic ◽

Artificial Environment ◽

3D Printed

Extracellular Vesicles (EVs) are considered promising nanoscale therapeutics for bone regeneration. To date, EVs are typically procured from cells on 2D tissue culture plastic, an artificial environment that limits cell growth and does not replicate in situ biochemical or biophysical conditions. This study investigated the potential of 3D printed titanium scaffolds coated with hydroxyapatite to promote the therapeutic efficacy of osteoblast-derived EVs. Ti6Al4V titanium scaffolds with different pore sizes (500 and 1000 µm) and shapes (square and triangle) were fabricated by selective laser melting. A bone-mimetic nano-needle hydroxyapatite (nnHA) coating was then applied. EVs were procured from scaffold-cultured osteoblasts over 2 weeks and vesicle concentration was determined using the CD63 ELISA. Osteogenic differentiation of human bone marrow stromal cells (hBMSCs) following treatment with primed EVs was evaluated by assessing alkaline phosphatase activity, collagen production and calcium deposition. Triangle pore scaffolds significantly increased osteoblast mineralisation (1.5-fold) when compared to square architectures (P ≤ 0.001). Interestingly, EV yield was also significantly enhanced on these higher permeability structures (P ≤ 0.001), in particular (2.2-fold) for the larger pore structures (1000 µm). Furthermore osteoblast-derived EVs isolated from triangular pore scaffolds significantly increased hBMSCs mineralisation when compared to EVs acquired from square pore scaffolds (1.7-fold) and 2D culture (2.2-fold) (P ≤ 0.001). Coating with nnHA significantly improved osteoblast mineralisation (>2.6-fold) and EV production (4.5-fold) when compared to uncoated scaffolds (P ≤ 0.001). Together, these findings demonstrate the potential of harnessing bone-mimetic culture platforms to enhance the production of pro-regenerative EVs as an acellular tool for bone repair.

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An in situ tissue engineering scaffold with growth factors combining angiogenesis and osteoimmunomodulatory functions for advanced periodontal bone regeneration

Journal of Nanobiotechnology ◽

10.1186/s12951-021-00992-4 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Tian Ding ◽

Wenyan Kang ◽

Jianhua Li ◽

Lu Yu ◽

Shaohua Ge

Keyword(s):

Tissue Engineering ◽

Growth Factors ◽

Bone Regeneration ◽

Bone Defect ◽

Bone Repair ◽

Macrophage Polarization ◽

Tissue Engineering Scaffold ◽

Bone Morphogenetic Protein 2 ◽

In Situ Tissue Engineering

Abstract Background The regeneration of periodontal bone defect remains a vital clinical challenge. To date, numerous biomaterials have been applied in this field. However, the immune response and vascularity in defect areas may be key factors that are overlooked when assessing the bone regeneration outcomes of biomaterials. Among various regenerative therapies, the up-to-date strategy of in situ tissue engineering stands out, which combined scaffold with specific growth factors that could mimic endogenous regenerative processes. Results Herein, we fabricated a core/shell fibrous scaffold releasing basic fibroblast growth factor (bFGF) and bone morphogenetic protein-2 (BMP-2) in a sequential manner and investigated its immunomodulatory and angiogenic properties during periodontal bone defect restoration. The in situ tissue engineering scaffold (iTE-scaffold) effectively promoted the angiogenesis of periodontal ligament stem cells (PDLSCs) and induced macrophage polarization into pro-healing M2 phenotype to modulate inflammation. The immunomodulatory effect of macrophages could further promote osteogenic differentiation of PDLSCs in vitro. After being implanted into the periodontal bone defect model, the iTE-scaffold presented an anti-inflammatory response, provided adequate blood supply, and eventually facilitated satisfactory periodontal bone regeneration. Conclusions Our results suggested that the iTE-scaffold exerted admirable effects on periodontal bone repair by modulating osteoimmune environment and angiogenic activity. This multifunctional scaffold holds considerable promise for periodontal regenerative medicine and offers guidance on designing functional biomaterials. Graphic Abstract

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In situ silk fibroin-mediated crystal formation of octacalcium phosphate and its application in bone repair

Materials Science and Engineering C ◽

10.1016/j.msec.2018.10.041 ◽

2019 ◽

Vol 95 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Fengxuan Han ◽

Yuanbin Hu ◽

Jiaying Li ◽

Jiawei Gong ◽

Qianping Guo ◽

...

Keyword(s):

Silk Fibroin ◽

Bone Repair ◽

Octacalcium Phosphate ◽

Crystal Formation

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Efficient drug delivery system for bone repair by tuning the surface of hydroxyapatite particles

RSC Advances ◽

10.1039/c6ra24551j ◽

2016 ◽

Vol 6 (107) ◽

pp. 104969-104978 ◽

Cited By ~ 12

Author(s):

Sobia Tabassum ◽

Saba Zahid ◽

Faiza Zarif ◽

Mazhar Amjad Gilani ◽

Faisal Manzoor ◽

...

Keyword(s):

Drug Delivery ◽

Drug Delivery System ◽

Surface Properties ◽

Functional Groups ◽

Bone Repair ◽

Precipitation Method ◽

Drug Delivery Vehicles ◽

Delivery Vehicles ◽

Co Precipitation

Efficient drug delivery vehicles, hydroxyapatite modified by carboxylic acids, were prepared by an in situ co-precipitation method. The presence of functional groups and subsequent surface properties of modified HA improved ibuprofen loading and release efficiency.

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