Temperature Dependence of the QyResonance Raman Spectra of Bacteriochlorophylls, the Primary Electron Donor, and Bacteriopheophytins in the Bacterial Photosynthetic Reaction Center†

Biochemistry ◽  
1997 ◽  
Vol 36 (28) ◽  
pp. 8559-8566 ◽  
Author(s):  
Nerine J. Cherepy ◽  
Andrew P. Shreve ◽  
Laura J. Moore ◽  
Steven G. Boxer ◽  
Richard A. Mathies
Keyword(s):  
Temperature Dependence ◽  
Reaction Center ◽  
Raman Spectra ◽  
Electron Donor ◽  
Photosynthetic Reaction Center ◽  
Primary Electron ◽  
Primary Electron Donor ◽  
Bacterial Photosynthetic Reaction Center

scholarly journals Switching sides—Reengineered primary charge separation in the bacterial photosynthetic reaction center

2019 ◽  
Vol 117 (2) ◽  
pp. 865-871 ◽  
Author(s):  
Philip D. Laible ◽  
Deborah K. Hanson ◽  
James C. Buhrmaster ◽  
Gregory A. Tira ◽  
Kaitlyn M. Faries ◽  
...  
Keyword(s):  
Reaction Center ◽  
Charge Separation ◽  
Internal Conversion ◽  
Photosynthetic Reaction Center ◽  
Primary Electron ◽  
Primary Electron Donor ◽  
Primary Charge Separation ◽  
Bacteriochlorophyll Dimer ◽  
Initial Charge ◽  
Bacterial Photosynthetic Reaction Center

We report 90% yield of electron transfer (ET) from the singlet excited state P* of the primary electron-donor P (a bacteriochlorophyll dimer) to the B-side bacteriopheophytin (HB) in the bacterial photosynthetic reaction center (RC). Starting from a platform Rhodobacter sphaeroides RC bearing several amino acid changes, an Arg in place of the native Leu at L185—positioned over one face of HB and only ∼4 Å from the 4 central nitrogens of the HB macrocycle—is the key additional mutation providing 90% yield of P+HB−. This all but matches the near-unity yield of A-side P+HA− charge separation in the native RC. The 90% yield of ET to HB derives from (minimally) 3 P* populations with distinct means of P* decay. In an ∼40% population, P* decays in ∼4 ps via a 2-step process involving a short-lived P+BB− intermediate, analogous to initial charge separation on the A side of wild-type RCs. In an ∼50% population, P* → P+HB− conversion takes place in ∼20 ps by a superexchange mechanism mediated by BB. An ∼10% population of P* decays in ∼150 ps largely by internal conversion. These results address the long-standing dichotomy of A- versus B-side initial charge separation in native RCs and have implications for the mechanism(s) and timescale of initial ET that are required to achieve a near-quantitative yield of unidirectional charge separation.

Influence of Electronic Asymmetry on the Spectroscopic and Photodynamic Properties of the Primary Electron Donor in the Photosynthetic Reaction Center

1996 ◽  
Vol 100 (44) ◽  
pp. 17696-17707 ◽  
Author(s):  
Laurent L. Laporte ◽  
Vaithianathan Palaniappan ◽  
Dianna G. Davis ◽  
Christine Kirmaier ◽  
Craig C. Schenck ◽  
...  
Keyword(s):  
Reaction Center ◽  
Electron Donor ◽  
Photosynthetic Reaction Center ◽  
Primary Electron ◽  
Primary Electron Donor

Symmetric Structural Features and Binding Site of the Primary Electron Donor in the Reaction Center of Chlorobium

Biochemistry ◽  
1995 ◽  
Vol 34 (35) ◽  
pp. 11099-11105 ◽  
Author(s):  
Ute Feiler ◽  
Delphine Albouy ◽  
Bruno Robert ◽  
Tony A. Mattioli
Keyword(s):  
Binding Site ◽  
Reaction Center ◽  
Electron Donor ◽  
Structural Features ◽  
Primary Electron ◽  
Primary Electron Donor

Electronic Couplings and Energy Transfer Dynamics in the Oxidized Primary Electron Donor of the Bacterial Reaction Center

2004 ◽  
Vol 108 (5) ◽  
pp. 1753-1765 ◽  
Author(s):  
Xanthipe J. Jordanides ◽  
Gregory D. Scholes ◽  
Warwick A. Shapley ◽  
Jeffrey R. Reimers ◽  
Graham R. Fleming
Keyword(s):  
Energy Transfer ◽  
Reaction Center ◽  
Electron Donor ◽  
Primary Electron ◽  
Primary Electron Donor ◽  
Bacterial Reaction Center
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