scholarly journals Differential Expression of Proteins in Response to Ceramide-Mediated Stress Signal in Colon Cancer Cells by 2-D Gel Electrophoresis and MALDI-TOF−MS

2005 ◽  
Vol 4 (3) ◽  
pp. 870-880 ◽  
Author(s):  
M. Fillet ◽  
C. Cren-Olivé ◽  
A.-F. Renert ◽  
J. Piette ◽  
F. Vandermoere ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Differential Expression ◽  
Gel Electrophoresis ◽  
Colon Cancer Cells ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Stress Signal ◽  
Tof Ms

Differential expression of nanog1 and nanogp8 in colon cancer cells

2012 ◽  
Vol 418 (2) ◽  
pp. 199-204 ◽  
Author(s):  
Tatsuya Ishiguro ◽  
Ai Sato ◽  
Hirokazu Ohata ◽  
Hiroaki Sakai ◽  
Hitoshi Nakagama ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Differential Expression ◽  
Colon Cancer Cells

scholarly journals Cell-Main Spectra Profile Screening Technique in Simulation of Circulating Tumour Cells Using MALDI-TOF Mass Spectrometry

Cancers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 3775
Author(s):  
Wararat Chiangjong ◽  
Sebastian Chakrit Bhakdi ◽  
Noppawan Woramongkolchai ◽  
Thitinee Vanichapol ◽  
Nutkridta Pongsakul ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cancer Cells ◽  
Cancer Metastasis ◽  
Primary Tumour ◽  
White Blood Cells ◽  
Low Frequency ◽  
Cell Types ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Circulating atypical cells (CAC) are released from a primary tumour site into peripheral blood and are indicators of cancer metastasis. CAC occur at very low frequency in circulating blood, and their detection remains challenging. Moreover, white blood cells (WBC) are the major contaminant in enriched CAC samples. Here, we developed matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) as a novel CAC characterization platform. Main spectra profiles (MSP) of normal and cancer cells were generated by MALDI-TOF MS, and a cell-main spectra database was then compiled and analysed using the MALDI Biotyper software. Logarithmic scores accurately predicted distinct cell types. The feasibility of this workflow was then validated using simulated samples, which were prepared by 5000 WBC of three healthy individuals spiked with varying numbers (3, 6, 12, 25, 50, and 100) of lung, colon, or prostate cancer cells. MALDI-TOF MS was able to detect cancer cells down to six cells over the background noise of 5000 WBC with significantly higher predictive scores as compared to WBC alone. Further development of cell-MSP database to cover all cancer types sourced from cell lines and patient tumours may enable the use of MALDI-TOF MS as a cancer-screening platform in clinical settings in the future.

scholarly journals Identification of Differential N-Glycan Compositions in the Serum and Tissue of Colon Cancer Patients by Mass Spectrometry

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 343
Author(s):  
Marcelo de M.A. Coura ◽  
Eder A. Barbosa ◽  
Guilherme D. Brand ◽  
Carlos Bloch ◽  
Joao B. de Sousa
Keyword(s):  
Mass Spectrometry ◽  
Colon Cancer ◽  
Cancer Patients ◽  
Total Serum ◽  
Healthy Controls ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Cancer Tumor ◽  
Glycan Profiling ◽  
Tof Ms

Colorectal cancer (CRC) ranks second as the leading cause of cancer-related deaths worldwide. N-glycosylation is one of the most common posttranslational protein modifications. Therefore, we studied the total serum N-glycome (TSNG) of 13 colon cancer patients compared to healthy controls using MALDI-TOF/MS and LC-MS. N-glycosylation of cancer tumor samples from the same cohort were further quantified using a similar methodology. In total, 23 N-glycan compositions were down-regulated in the serum of colon cancer patients, mostly galactosylated forms whilst the mannose-rich HexNAc2Hex7, the fucosylated bi-antennary glycan HexNAc4Hex5Fuc1NeuAc2, and the tetra-antennary HexNAc6Hex7NeuAc3 were up-regulated in serum. Hierarchical clustering analysis of TSNG correctly singled out 85% of the patients from controls. Albeit heterogenous, N-glycosylation of tumor samples showed overrepresented oligomannosidic, bi-antennary hypogalactosylated, and branched compositions related to normal colonic tissue, in both MALDI-TOF/MS and LC-MS analysis. Moreover, compositions found upregulated in tumor tissue were mostly uncorrelated to compositions in serum of cancer patients. Mass spectrometry-based N-glycan profiling in serum shows potential in the discrimination of patients from healthy controls. However, the compositions profile in serum showed no parallel with N-glycans in tumor microenvironment, which suggests a different origin of compositions found in serum of cancer patients.

Application of nanoLC-ESI-TOF-MS for the metabolomic analysis of phenolic compounds from extra-virgin olive oil in treated colon-cancer cells

2012 ◽  
Vol 63 ◽  
pp. 128-134 ◽  
Author(s):  
S. Fernández-Arroyo ◽  
A. Gómez-Martínez ◽  
L. Rocamora-Reverte ◽  
R. Quirantes-Piné ◽  
A. Segura-Carretero ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Phenolic Compounds ◽  
Olive Oil ◽  
Cancer Cells ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Metabolomic Analysis ◽  
Colon Cancer Cells ◽  
Tof Ms

scholarly journals UHMK1 Dependent Phosphorylation of Cajal Body Protein Coilin Altered 5-FU Sensitivity in Colon Cancer Cells

2021 ◽  
Author(s):  
Huan Niu ◽  
Meng Zhao ◽  
Jing Huang ◽  
Jing Wang ◽  
Yang Si ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Cajal Body ◽  
Rna Seq ◽  
Colon Cancer Cells ◽  
Body Protein ◽  
Cell Functions ◽  
Stress Signal ◽  
Response To Stress ◽  
Nuclear Structures

Abstract Background: Resistance to 5-fluorouracil (5-FU) in chemotherapy and recurrence of colorectal tumors is a serious problem to be resolved for the improvement of clinical outcomes.Methods: In the present study, the effects of conditioned medium (CM) derived from 5-FU-resistant HCT-8/FU on cell functions were evaluated. The methods of immunofluorescence and RNA-seq analyses were used to investigate the molecular mechanism underlining the roles of CM from resistant cells.Results: we found that CM derived from 5-FU-resistant HCT-8/FU was able to reduce 5-FU chemosensitivity of HCT-8 colon cancer cells, with correlating changes in the number and morphology of the Cajal bodies (CBs) as observable nuclear structures. We identified UHMK1 was able to change the disassembly and reassembly of CBs regulated by the phosphorylation of coilin, a major component of CBs, and subsequently resulted in a large number of variations of RNA alternative splicing, affecting the cell survival following 5-FU treatment through changes in intracellular phenotype and transmitted preadaptive signals to adjacent cells in tumor microenvironment (TME).Conclusion: Our finding provided evidence to demonstrate CBs of their disassembling/reassembling dynamics to indicate drug sensitivity or resistance in tumor cells in response to stress signal. The results also suggested that UHMK1 could be an important factor to maintain CB structure and morphology with its possible roles in the regulation of splicing events, especially when cells exposed to cytotoxic drugs.

Sign in / Sign up

Export Citation Format

Share Document