Liver Histological Improvement After Administration of High-Dose Vitamin C in Guinea Pig with Nonalcoholic Steatohepatitis

2018 ◽  
Vol 88 (5-6) ◽  
pp. 263-269
Author(s):  
Seong-Hoon Park ◽  
A Lum Han ◽  
Na-Hyung Kim ◽  
Sae-Ron Shin

Abstract. Background: Vitamin C is a strong antioxidant, and the health effects of vitamin C megadoses have not been validated despite the apparent health benefits. Therefore, the present study sought to confirm the effects of vitamin C megadoses. Materials and Methods : Four groups of six guinea pigs were used. Each group was fed one of the following diets for three weeks: normal diet, methionine choline-deficient diet, methionine choline-deficient diet + vitamin C megadose (MCD + vit C 2.5 g/kg/day), and methionine-choline deficient diet + ursodeoxycholic acid (MCD + UDCA 30 mg/kg/day). The MCD diet was given to induce nonalcoholic steatohepatitis, and UDCA was used to treat nonalcoholic steatohepatitis. Three weeks after initial diet administration, the results of biochemical tests and liver biopsy were compared between the groups. Results: The cytoplasm state was similar in the MCD + vit C and MCD + UDCA groups, exhibiting clearing of the cytoplasm and ballooning degeneration. However, macrovesicular steatosis was not observed in the MCD + vit C group. Aspartate transaminase and alanine transaminase were elevated significantly following vitamin C administration. Conclusions: The present study confirmed that alone vitamin C megadoses are potential remedies for nonalcoholic steatohepatitis, based on the liver biopsy results of guinea pigs that were unable to synthesize vitamin C.

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 953
Author(s):  
Vitor Teixeira ◽  
Ibrahim Mohamed ◽  
Jean-Claude Lavoie

Premature neonates are submitted to an early-life oxidative stress from parenteral nutrition, which is vitamin C (VC) deficient and induces low endogenous levels of glutathione. The oxidative stress caused by these deficiencies may permanently affect liver glycolysis and lipogenesis. This study evaluates the short- and long-term effects of neonatal VC and cysteine deficient diets on redox and energy metabolism. Three-day-old Hartley guinea pigs from both sexes were given a regular or a deficient diet (VC, cysteine, or both) until week 1 of life. Half of the animals were sacrificed at this age, while the other half ate a complete diet until 12 weeks. Liver glutathione and the activity and protein levels of glucokinase, phosphofructokinase, and acetyl-CoA-carboxylase were measured. Statistics: factorial ANOVA (5% threshold). At 1 week, all deficient diets decreased glutathione and the protein levels of glucokinase and phosphofructokinase, while cysteine deficiency decreased acetyl-CoA-carboxylase levels. A similar enzyme level was observed in control animals at 12 weeks. At this age, VC deficiency decreased glutathione, while cysteine increased it. Acetyl-CoA-carboxylase protein levels were increased, which decreased its specific activity. Early-life VC and cysteine deficiencies induce neonatal oxidative stress and an adult-like metabolism, while predisposing to increased lipogenic rates during adulthood.


2011 ◽  
Vol 35 (1) ◽  
pp. 141-152
Author(s):  
Jamal Khalaf Atiyah AL-Faragi

One hundred and twelve Common carp fish fingerling Cyprinus carpio L.were collected, range between 25-28 gm in weight to study the effect of different concentrations of local probiotic and mixed with Vitamin C in concentration 1g/kg feed on growth performance, blood and biochemical picture. This experiment included 7 groups each group contain 16 fish distributed randomly as follow: Control group (C ) fed diet free vitamin C and probiotic during the period of 60 days and all total protin in feed reach 26% . Group 2(T1) group fed diet contain Vit C (1gm /kg) food. Treated (T2andT3) groups fed diet containing probiotic 4.5&5.5 g/ kg respectively , where as groupsT4,T5 and T6 fed the diets containing Probiotic added at level 4.5,5.5 and 6.5 g/ kg respectively and each mixed with 1 gm\kg Vit C .Results of fish total growth rate were 6.86,9.82,15.00,10.87,17.51,14.60 and 13.51gm, evaluate relative growth rate ,the absolute specific growth rate, food conversion and the protein efficiency ratio . The statistically analysis showed increase significantly(P≤0.05)for T4 in all treatment. While erythrocyte counts was 1.80, 1.88, 1.95, 2.40, 2.00, 2.70, 2.20 and 2.10 ×106 cells\mm3, blood cells counts are 24.40 ,24.60 ,25.80 ,27.00 ,26.00 ,27.96 ,26.60 and 26.20×103cells\mm3, blood haemoglobin was8.40,8.70,9.80, 11.10,10.20,11.90,10.80 and 10.50 gm\100dl, haematocrit value are 27.10 ,27.60 ,28.50 ,29.90 ,29.10 ,30.50 ,29.50 and29.30 % for fish before starting expermint (Z) and C,T1,T2,T3,T4,T5,T6,groups, respectively . Evaluate the enzymatic activity for AST,ALT and ALP in blood serum of fish . The statisticaly analysis showed decrease significantly(P≤0.05) at treatment contain mix probiotic and Vit C but excel T4 inform 70.40,10.40,30.30 I U\ L respectively.Conclusion ,the present studied suggested that the level of probiotic and Vit C (4.5g\kg and Vi C 1gm\kg) used could be improve the performance growth and fish healthy.


2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Graziella Rodrigues ◽  
Andrea Janz Moreira ◽  
Silvia Bona ◽  
Elizângela Schemitt ◽  
Cláudio Augusto Marroni ◽  
...  

Objective. In this study, we evaluated the efficacy of simvastatin in the treatment of nonalcoholic steatohepatitis induced by methionine and choline-deficient diet in mice and its possible effect on factors involved in the pathogenesis of the disease including oxidative stress and endoplasmic reticulum stress. Method. Male C57BL6 mice were fed either a normal diet (control) or a methionine and choline-deficient diet for four weeks and then treated orally with simvastatin (4 mg/kg once a day) for two final weeks. At the end of the experimental period, liver integrity, biochemical analysis, hepatic lipids, histology, DNA damage, biomarkers of oxidative stress, and endoplasmic reticulum stress were assessed. Results. Simvastatin treatment was able to significantly reduce hepatic damage enzymes and hepatic lipids and lower the degree of hepatocellular ballooning, without showing genotoxic effects. Simvastatin caused significant decreases in lipid peroxidation, with some changes in antioxidant enzymes superoxide dismutase and glutathione peroxidase. Simvastatin activates antioxidant enzymes via Nrf2 and inhibits endoplasmic reticulum stress in the liver. Conclusions. In summary, the results provide evidence that in mice with experimental nonalcoholic steatohepatitis induced by a methionine and choline-deficient diet, the reduction of liver damage by simvastatin is associated with attenuated oxidative and endoplasmic reticulum stress.


2020 ◽  
Vol 54 ◽  
Author(s):  
Marc Evans M. Abat ◽  
Cristina Larracas ◽  
Ian Theodore G. Cabaluna

KEY FINDINGS There is no direct evidence available as of this point for efficacy of intravenous vitamin C as an adjunctive treatment in preventing mortality or shortening disease course among adults suspected of, or positive for COVID-19. • Vitamin C is currently not mentioned in the treatment guidelines for COVID. • Currently, there are 3 ongoing trials registered in clinicaltrials.gov studying intravenous vitamin C in COVID-19. No other ongoing or planned trials were registered in the other trial registries. • Most of the available data are from studies on disease populations which may be considered as COVID-19 suspects:  Conflicting results on mortality from indirect evidence among patients with sepsis with or without ARDS with significant reduction in mortality found in only a small subset of patients (n-40) with severe sepsis given high dose Vitamin C infusion.  Strong evidence supporting no mortality benefit from 5 meta-analyses on critically ill patients due to conditions other than or in combination with sepsis who were given Vit C infusion alone or in combinations with other medications. One meta-analysis showed benefit in decreasing duration of ICU stay and mechanical ventilation but sample size is small. Most showed no benefit on and other key endpoints such as acute kidney injury, duration of hospital stay/ ICU stay/ duration of vasopressor use or duration of mechanical ventilation. • The use of Vit C infusion is not mentioned in the treatment guidelines for COVID-19 or ARDS. • The risks or adverse events with short term use of Vitamin C infusion in the general population is negligible or minimal. It should be avoided in patients with G6PD insufficiency. The dose should be carefully adjusted for patients with renal insufficiency.


Neuroscience ◽  
1997 ◽  
Vol 77 (1) ◽  
pp. 167-174 ◽  
Author(s):  
M Revuelta ◽  
M Romero-Ramos ◽  
J.L Venero ◽  
F Millan ◽  
A Machado ◽  
...  

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