eSGA: E. coli synthetic genetic array analysis

2008 ◽  
Vol 5 (9) ◽  
pp. 789-795 ◽  
Author(s):  
Gareth Butland ◽  
Mohan Babu ◽  
J Javier Díaz-Mejía ◽  
Fedyshyn Bohdana ◽  
Sadhna Phanse ◽  
...  
Keyword(s):  
Synthetic Genetic Array ◽  
Array Analysis ◽  
E Coli

eSGA: E. coli Synthetic Genetic Array analysis

2008 ◽  
Author(s):  
Gareth Butland ◽  
Mohan Babu ◽  
Jack Greenblatt ◽  
Andrew Emili
Keyword(s):  
Synthetic Genetic Array ◽  
Array Analysis ◽  
E Coli

Synthetic Genetic Array Analysis

2016 ◽  
Vol 2016 (4) ◽  
pp. pdb.prot088807 ◽  
Author(s):  
Elena Kuzmin ◽  
Michael Costanzo ◽  
Brenda Andrews ◽  
Charles Boone
Keyword(s):  
Synthetic Genetic Array ◽  
Array Analysis

scholarly journals Synthetic Genetic Array Analysis in Saccharomyces cerevisiae Provides Evidence for an Interaction Between RAT8/DBP5 and Genes Encoding P-Body Components

Genetics ◽  
2008 ◽  
Vol 179 (4) ◽  
pp. 1945-1955 ◽  
Author(s):  
John J. Scarcelli ◽  
Susan Viggiano ◽  
Christine A. Hodge ◽  
Catherine V. Heath ◽  
David C. Amberg ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Synthetic Genetic Array ◽  
Array Analysis ◽  
Genes Encoding ◽  
Body Components

Synthetic Genetic Array Analysis for Global Mapping of Genetic Networks in Yeast

2014 ◽  
pp. 143-168 ◽  
Author(s):  
Elena Kuzmin ◽  
Sara Sharifpoor ◽  
Anastasia Baryshnikova ◽  
Michael Costanzo ◽  
Chad L. Myers ◽  
...  
Keyword(s):  
Genetic Networks ◽  
Synthetic Genetic Array ◽  
Array Analysis ◽  
Global Mapping

Secretory pathway genes assessed by high-throughput microscopy and synthetic genetic array analysis

2011 ◽  
Vol 7 (9) ◽  
pp. 2589 ◽  
Author(s):  
Peter W. Bircham ◽  
David R. Maass ◽  
Christina A. Roberts ◽  
Poh Y. Kiew ◽  
Yee S. Low ◽  
...  
Keyword(s):  
High Throughput ◽  
Secretory Pathway ◽  
Synthetic Genetic Array ◽  
Array Analysis

scholarly journals Clathrin light chain directs endocytosis by influencing the binding of the yeast Hip1R homologue, Sla2, to F-actin

2011 ◽  
Vol 22 (19) ◽  
pp. 3699-3714 ◽  
Author(s):  
Douglas R. Boettner ◽  
Helena Friesen ◽  
Brenda Andrews ◽  
Sandra K. Lemmon
Keyword(s):  
Heavy Chain ◽  
Light Chain ◽  
Deletion Mutation ◽  
Actin Binding ◽  
Synthetic Genetic Array ◽  
Array Analysis ◽  
Clathrin Heavy Chain ◽  
N Terminus ◽  
Mechanistic Basis

The role of clathrin light chain (CLC) in clathrin-mediated endocytosis is not completely understood. Previous studies showed that the CLC N-terminus (CLC-NT) binds the Hip1/Hip1R/Sla2 family of membrane/actin–binding factors and that overexpression of the CLC-NT in yeast suppresses endocytic defects of clathrin heavy-chain mutants. To elucidate the mechanistic basis for this suppression, we performed synthetic genetic array analysis with a clathrin CLC-NT deletion mutation (clc1-Δ19-76). clc1-Δ19-76 suppressed the internalization defects of null mutations in three late endocytic factors: amphiphysins (rvs161 and rvs167) and verprolin (vrp1). In actin sedimentation assays, CLC binding to Sla2 inhibited Sla2 interaction with F-actin. Furthermore, clc1-Δ19-76 suppression of the rvs and vrp phenotypes required the Sla2 actin-binding talin-Hip1/R/Sla2 actin-tethering C-terminal homology domain, suggesting that clc1-Δ19-76 promotes internalization by prolonging actin engagement by Sla2. We propose that CLC directs endocytic progression by pruning the Sla2-actin attachments in the clathrin lattice, providing direction for membrane internalization.

τ-SGA: synthetic genetic array analysis for systematically screening and quantifying trigenic interactions in yeast

2021 ◽  
Vol 16 (2) ◽  
pp. 1219-1250
Author(s):  
Elena Kuzmin ◽  
Mahfuzur Rahman ◽  
Benjamin VanderSluis ◽  
Michael Costanzo ◽  
Chad L. Myers ◽  
...  
Keyword(s):  
Synthetic Genetic Array ◽  
Array Analysis
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