Structure and function differences in the prelimbic cortex to basolateral amygdala circuit mediate trait vulnerability in a novel model of acute social defeat stress in male mice

2021 ◽  
Author(s):  
Yael S. Grossman ◽  
Clementine Fillinger ◽  
Alessia Manganaro ◽  
George Voren ◽  
Rachel Waldman ◽  
...  
Keyword(s):  
Basolateral Amygdala ◽  
Social Defeat ◽  
Structure And Function ◽  
Prelimbic Cortex ◽  
Male Mice ◽  
Social Defeat Stress ◽  
And Function ◽  
Novel Model

scholarly journals Prelimbic-amygdala overexcitability mediates trait vulnerability in a novel mouse model of acute social defeat stress

2020 ◽  
Author(s):  
Yael S. Grossman ◽  
Clementine Fillinger ◽  
Alessia Manganaro ◽  
George Voren ◽  
Rachel Waldman ◽  
...  
Keyword(s):  
Structure Function ◽  
Social Stress ◽  
Basolateral Amygdala ◽  
Control Level ◽  
Behavioral Responses ◽  
Social Defeat ◽  
Confocal Imaging ◽  
Spine Density ◽  
Male Mice ◽  
Social Defeat Stress

BACKGROUNDDepression is a debilitating neuropsychiatric disorder with 20% lifetime prevalence in the developed world but only approximately half of afflicted individuals respond to currently available therapies. While there is growing understanding of the neurobiological underpinnings of the depressed brain, much less is known about the preexisting circuitry leading to selective vulnerability versus resilience. Elucidating these networks could lead to novel preventative approaches.METHODSWe developed a model of acute social defeat stress (ASDS) that allows classification of male mice into “susceptible” (socially avoidant) versus “resilient” (expressing control-level social approach) one hour after exposure to six minutes of social stress. Using circuit tracing and high-resolution confocal imaging, we explored differences in activation and dendritic spine density and morphology in the prelimbic to basolateral amygdala (PL→BLA) circuit in resilient versus susceptible mice. To test the functional relevance of identified structure/function differences to divergent behavioral responses, we used an intersectional chemogenetic approach to inhibit the PL→BLA circuit during or prior to ASDS.RESULTSSusceptible mice had greater PL→BLA recruitment during ASDS and activated PL→BLA neurons from susceptible mice had more and larger mushroom spines compared to resilient mice. Inhibition of the PL→BLA circuit led to a population shift towards resilience.CONCLUSIONPreexisting PL→BLA structure/function differences mediate divergent behavioral responses to ASDS in male mice. These results support the PL→BLA circuit as a biomarker of trait vulnerability and potential target for prevention of stress-induced psychopathology.

Comparative effects of Orchis anatolica vs. the red Korean Panax ginseng treatments on testicular structure and function of adult male mice

2015 ◽  
Vol 12 (1) ◽  
Author(s):  
Nabil A. Khouri ◽  
Haytham M. Daradka ◽  
Mohammed Z. Allouh ◽  
Ahmad S. Alkofahi
Keyword(s):  
Panax Ginseng ◽  
Male Fertility ◽  
Virgin Female ◽  
Reproductive Organs ◽  
Serum Markers ◽  
Structure And Function ◽  
Control Group ◽  
Male Mice ◽  
Fertility Potential ◽  
And Function

Abstract: The effects of: Both plants were administered orally to two separate mice groups at a dose of 800 mg/kg/day for 35 days and compared with control group. After treatment, 5 mice of each group were sacrificed and total mice weights, reproductive organs’ weights, spermatogenesis, and androgenic serum markers were investigated. The remaining mice from all groups were allowed to mate with virgin female mice to explore male fertility potential.: Results indicated that body and organs’ weights were increased significantly in mice treated with: We can conclude that

scholarly journals A novel model to characterize structure and function of BRCA1

2017 ◽  
Vol 42 (1) ◽  
pp. 34-44 ◽  
Author(s):  
Dong Lin ◽  
Reza Izadpanah ◽  
Stephen E. Braun ◽  
Eckhard Alt
Keyword(s):  
Structure And Function ◽  
And Function ◽  
Novel Model

scholarly journals Unique effects of social defeat stress in adolescent male mice on the Netrin-1/DCC pathway, prefrontal cortex dopamine and cognition (Social stress in adolescent vs. adult male mice)

eNeuro ◽  
2021 ◽  
pp. ENEURO.0045-21.2021
Author(s):  
Philip Vassilev ◽  
Andrea Haree Pantoja-Urban ◽  
Michel Giroux ◽  
Dominique Nouel ◽  
Giovanni Hernandez ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Adult Male ◽  
Social Stress ◽  
Social Defeat ◽  
Adolescent Male ◽  
Male Mice ◽  
Social Defeat Stress

scholarly journals Dysfunction in Ribosomal Gene Expression in the Hypothalamus and Hippocampus following Chronic Social Defeat Stress in Male Mice as Revealed by RNA-Seq

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Dmitry A. Smagin ◽  
Irina L. Kovalenko ◽  
Anna G. Galyamina ◽  
Anatoly O. Bragin ◽  
Yuriy L. Orlov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Social Stress ◽  
Ribosome Biogenesis ◽  
Social Defeat ◽  
Brain Regions ◽  
Ribosomal Gene ◽  
Rna Seq ◽  
Male Mice ◽  
Social Defeat Stress ◽  
Chronic Social Defeat Stress

Chronic social defeat stress leads to the development of anxiety- and depression-like states in male mice and is accompanied by numerous molecular changes in brain. The influence of 21-day period of social stress on ribosomal gene expression in five brain regions was studied using the RNA-Seq database. MostRps, Rpl, Mprs, andMprlgenes were upregulated in the hypothalamus and downregulated in the hippocampus, which may indicate ribosomal dysfunction following chronic social defeat stress. There were no differentially expressed ribosomal genes in the ventral tegmental area, midbrain raphe nuclei, or striatum. This approach may be used to identify a pharmacological treatment of ribosome biogenesis abnormalities in the brain of patients with “ribosomopathies.”

Changes in the expression of dopaminergic genes in brain structures of male mice exposed to chronic social defeat Stress: An RNA-seq study

2016 ◽  
Vol 50 (1) ◽  
pp. 161-163 ◽  
Author(s):  
I. L. Kovalenko ◽  
D. A. Smagin ◽  
A. G. Galyamina ◽  
Yu. L. Orlov ◽  
N. N. Kudryavtseva
Keyword(s):  
Social Defeat ◽  
Brain Structures ◽  
Rna Seq ◽  
Male Mice ◽  
Social Defeat Stress ◽  
Chronic Social Defeat Stress ◽  
Dopaminergic Genes

scholarly journals Female mice respond to male ultrasonic ‘songs’ with approach behaviour

2009 ◽  
Vol 5 (5) ◽  
pp. 589-592 ◽  
Author(s):  
K. Hammerschmidt ◽  
K. Radyushkin ◽  
H. Ehrenreich ◽  
J. Fischer
Keyword(s):  
Male Mouse ◽  
Experimental Situation ◽  
Structure And Function ◽  
Ultrasonic Vocalizations ◽  
Male Mice ◽  
Playback Experiments ◽  
Female Mice ◽  
And Function ◽  
Female Responses ◽  
Artificial Control

The ultrasonic vocalizations of mice are attracting increasing attention, because they have been recognized as an informative readout in genetically modified strains. In addition, the observation that male mice produce elaborate sequences of ultrasonic vocalizations (‘song’) when exposed to female mice or their scents has sparked a debate as to whether these sounds are—in terms of their structure and function—analogous to bird song. We conducted playback experiments with cycling female mice to explore the function of male mouse songs. Using a place preference design, we show that these vocalizations elicited approach behaviour in females. In contrast, the playback of whistle-like artificial control sounds did not evoke approach responses. Surprisingly, the females also did not respond to pup isolation calls. In addition, female responses did not vary in relation to reproductive cycle, i.e. whether they were in oestrus or not. Furthermore, our data revealed a rapid habituation of subjects to the experimental situation, which stands in stark contrast to other species' responses to courtship vocalizations. Nevertheless, our results clearly demonstrate that male mouse songs elicit females' interest.

scholarly journals Altered Structure and Function of Reproductive Organs in Transgenic Male Mice Overexpressing Human Aromatase*

Endocrinology ◽  
2001 ◽  
Vol 142 (6) ◽  
pp. 2435-2442 ◽  
Author(s):  
Xiangdong Li ◽  
Elina Nokkala ◽  
Wei Yan ◽  
Tomi Streng ◽  
Niina Saarinen ◽  
...  
Keyword(s):  
Reproductive Organs ◽  
Structure And Function ◽  
Male Mice ◽  
Human Aromatase ◽  
And Function ◽  
Transgenic Male

scholarly journals Chronic stress impairs male spermatogenesis function and nectin-3 protein expression in the testis

2020 ◽  
pp. 297-306
Author(s):  
T. Li ◽  
J. Yao ◽  
Q. Zhang ◽  
Q. Li ◽  
J. Li ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Molecular Mechanisms ◽  
Social Defeat ◽  
Histological Structure ◽  
Male Mice ◽  
Corticotropin Releasing Hormone ◽  
Social Defeat Stress ◽  
Control Male ◽  
Releasing Hormone ◽  
Chronic Social Defeat Stress

Chronic stress is a crucial public issue that occurs when a person is repetitively stimulated by various stressors. Previous researches have reported that chronic stress induces spermatogenesis dysfunction in the reproductive system, but its molecular mechanisms remain unclear. The nectin protein family, including nectin-1 to nectin-4, is Ca(2+)-independent immunoglobulin-like cell adhesion molecules, that are widely expressed in the hippocampus, testicular tissue, epithelial cells and other sites. Nectin-3 contributes to the sperm development at the late stage, and the abnormal expression of nectin-3 impairs spermatogenesis. Some recent studies have demonstrated that stress induces a decrease in nectin-3 expression in the hippocampus via corticotropin-releasing hormone (CRH) to corticotropin-releasing hormone receptor 1 (CRHR1) pathway. Here, we tested whether chronic stress also caused a reduction in nectin-3 expression in the testis. We established a chronic social defeat stress paradigm, which provides naturalistic and complex chronic stress in male C57BL/6 mice. After 25 days of chronic social defeat stress, the mice showed weight loss, thymic atrophy and some other typical symptoms of chronic stress (e.g. anxiety-like behavior and social avoidance behavior). We found gonad atrophy, testicular histological structure changes and semen quality reductions in the stressed mice. The stressed male mice significantly spent more time to impregnate the female mice than the control male mice. Moreover, nectin-3 protein levels in stressed mice were significantly decreased in the testes compared with those in control mice. In addition, we found that the CRHR1 expression level was increased in the testes of stressed mice. Therefore, we demonstrated a decreased level of nectin-3 expression and an increase in CRHR1 expression in the testis after exposure to chronic stress, which may provide a potential therapeutic target for the spermatogenesis dysfunction induced by chronic stress.

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