scholarly journals Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Sami Abu Hamdeh ◽  
Ganna Shevchenko ◽  
Jia Mi ◽  
Sravani Musunuri ◽  
Jonas Bergquist ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue

Regional mechanical properties of human brain tissue for computational models of traumatic brain injury

2017 ◽  
Vol 55 ◽  
pp. 333-339 ◽  
Author(s):  
John D. Finan ◽  
Sowmya N. Sundaresh ◽  
Benjamin S. Elkin ◽  
Guy M. McKhann ◽  
Barclay Morrison
Keyword(s):  
Mechanical Properties ◽  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Human Brain ◽  
Brain Tissue ◽  
Computational Models ◽  
Human Brain Tissue

scholarly journals TMEM119 as a specific marker of microglia reaction in traumatic brain injury in postmortem examination

2020 ◽  
Vol 134 (6) ◽  
pp. 2167-2176
Author(s):  
Simone Bohnert ◽  
Anja Seiffert ◽  
Stefanie Trella ◽  
Michael Bohnert ◽  
Luitpold Distel ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Survival Time ◽  
Brain Tissue ◽  
Causes Of Death ◽  
Cell Number ◽  
Specific Marker ◽  
Human Brain Tissue ◽  
Tissue Samples ◽  
Forensic Assessments

Abstract The aim of the present study was a refined analysis of neuroinflammation including TMEM119 as a useful microglia-specific marker in forensic assessments of traumatic causes of death, e.g., traumatic brain injury (TBI). Human brain tissue samples were obtained from autopsies and divided into cases with lethal TBI (n = 25) and subdivided into three groups according to their trauma survival time and compared with an age-, gender-, and postmortem interval-matched cohort of sudden cardiovascular fatalities as controls (n = 23). Brain tissue samples next to cortex contusions and surrounding white matter as well as samples of the ipsilateral uninjured brain stem and cerebellum were collected and stained immunohistochemically with antibodies against TMEM119, CD206, and CCR2. We could document the highest number of TMEM119-positive cells in acute TBI death with highly significant differences to the control numbers. CCR2-positive monocytes showed a significantly higher cell count in the cortex samples of TBI cases than in the controls with an increasing number of immunopositive cells over time. The number of CD206-positive M2 microglial cells increased survival time-dependent. After 3 days of survival, the cell number increased significantly in all four regions investigated compared with controls. In sum, we validate a specific and robustly expressed as well as fast reacting microglia marker, TMEM119, which distinguishes microglia from resident and infiltrating macrophages and thus offers a great potential for the estimation of the minimum survival time after TBI.

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