scholarly journals Sensitivity of Colorectal Cancer to Arginine Deprivation Therapy is Shaped by Differential Expression of Urea Cycle Enzymes

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Constantinos Alexandrou ◽  
Saif Sattar Al-Aqbi ◽  
Jennifer A. Higgins ◽  
William Boyle ◽  
Ankur Karmokar ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Differential Expression ◽  
Urea Cycle ◽  
Arginine Deprivation ◽  
Urea Cycle Enzymes

scholarly journals Carnitine administration to juvenile visceral steatosis mice corrects the suppressed expression of urea cycle enzymes by normalizing their transcription.

1992 ◽  
Vol 267 (8) ◽  
pp. 5032-5035
Author(s):  
M Horiuchi ◽  
K Kobayashi ◽  
M Tomomura ◽  
M Kuwajima ◽  
Y Imamura ◽  
...  
Keyword(s):  
Urea Cycle ◽  
Urea Cycle Enzymes

scholarly journals Differential expression of alternatively spliced transcripts related to energy metabolism in colorectal cancer

BMC Genomics ◽  
2016 ◽  
Vol 17 (S14) ◽  
Author(s):  
Anastasiya Vladimirovna Snezhkina ◽  
George Sergeevich Krasnov ◽  
Andrew Rostislavovich Zaretsky ◽  
Alex Zhavoronkov ◽  
Kirill Mikhailovich Nyushko ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Energy Metabolism ◽  
Differential Expression ◽  
Alternatively Spliced

Characterization of Alternatively Spliced Isoforms of MUC4 and ADAM12 Genes in a Metastatic Colorectal Cancer Cell Line Model

2021 ◽  
Author(s):  
Saleh Althenayyan ◽  
Mohammed H AlMuhanna ◽  
Abdulkareem Al Abdulrahman ◽  
Bandar Alghanem ◽  
Suliman A. Alsagaby ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Cell Line ◽  
Differential Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Colorectal Cancer Cell ◽  
Line Model ◽  
Cell Line Model ◽  
Alternatively Spliced

Colorectal cancer prognosis get worse with advancement of disease into metastatic stage. There is a pertinent need to develop prognostic biomarkers that can be used for personalized and precision medicine. Alternative splicing provides an insight into understanding of changes at isoform expression level which may not be evident at gene level. In this direction, we utilized our prior knowledge about significant alternatively spliced genes and chose ADAM12 and MUC4 for further characterization in a metastatic cell line model. These genes were found to be good prognostic indicators in The Cancer Genome Atlas database. We studied the gene organization and designed primers to specifically amplify a group of isoforms. Differential expression of these group of isoforms was observed in normal, primary and metastatic colorectal cancer cell lines. We further validated the results using sanger sequencing. Isoform expression was found to respond to the 5-fluorouracil treatment. RNAseq analysis of the cell lines further validated the differential expression of gene isoforms. Successful detection of ADAM12 and MUC4 in cell lysates varied according to the antibody used which may reflect differential expression of isoforms. This comprehensive study underscores the importance of studying alternatively spliced isoforms and their probable used as prognostic or predictive biomarkers.

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