scholarly journals Comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Camille Fédou ◽  
Benjamin Breuil ◽  
Igor Golovko ◽  
Stéphane Decramer ◽  
Pedro Magalhães ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Biomarker Discovery ◽  
Posterior Urethral Valves ◽  
Proof Of Concept ◽  
Renal Survival ◽  
Third Trimester ◽  
Urethral Valves ◽  
Endogenous Peptides ◽  
The Third ◽  
Fetal Urine

AbstractProduction of amniotic fluid (AF) is view as predominately driven by excretion of fetal urine (FU). However, the origin of AF peptides, often considered as potential biomarkers of developmental diseases, has never been investigated. Here, we evaluated the FU origin of AF peptides and if the AF peptide content can be used as a surrogate of FU. The abundance of endogenous peptides was analyzed by capillary electrophoresis coupled to mass spectrometry in 216 AF and 64 FU samples. A total of 2668 and 3257 peptides was found in AF and FU respectively. The AF peptidome largely overlapped with the FU peptidome, ranging from 54% in the second pregnancy trimester to 65% in the third trimester. Examination of a subset of 16 paired AF and FU samples revealed that 67 peptides displayed a significant positively correlated abundance in AF and FU, strongly suggesting that their presence in AF was directly associated to FU excretion. As proof-of-concept we showed that measuring the AF abundance of these 67 peptides of FU origin allowed prediction of postnatal renal survival in fetuses with posterior urethral valves. These results demonstrate that the AF peptidome can be considered as a good surrogate of the FU peptidome.

Laser Ablation of Posterior Urethral Valves by Fetal Cystoscopy

2015 ◽  
Vol 37 (4) ◽  
pp. 267-273 ◽  
Author(s):  
José María Martínez ◽  
Narcis Masoller ◽  
Roland Devlieger ◽  
Esther Passchyn ◽  
Olga Gómez ◽  
...  
Keyword(s):  
Renal Failure ◽  
Laser Ablation ◽  
Amniotic Fluid ◽  
Gestational Age ◽  
Significant Risk ◽  
Operation Time ◽  
Posterior Urethral Valves ◽  
Consecutive Series ◽  
Urethral Valves ◽  
Median Operation Time

Objective: To report the results of fetal cystoscopic laser ablation of posterior urethral valves (PUV) in a consecutive series in two referral centers. Methods: Twenty pregnant women with a presumptive isolated PUV were treated with fetal cystoscopy under local anesthesia. Identification and fulguration of the PUV by one or several firing-contacts with diode laser were attempted. Perinatal and long-term outcomes were prospectively recorded. Results: The median gestational age at procedure was 18.1 weeks (range 15.0-25.6), and median operation time was 24 min (range 15-40). Access to the urethra was achieved in 19/20 (95%) cases, and postoperative, normalization of bladder size and amniotic fluid was observed in 16/20 (80%). Overall, there were 9 (45%) terminations of pregnancy and 11 women (55%) delivered a liveborn baby at a mean gestational age of 37.3 (29.1-40.2) weeks. No infants developed pulmonary hypoplasia and all were alive at 15-110 months. Eight (40% of all fetuses, 72.7% of newborns) had normal renal function and 3 (27.3%) had renal failure awaiting renal transplantation. Conclusion: Fetoscopic laser ablation for PUV can achieve bladder decompression and amniotic fluid normalization with a single procedure in selected cases with anyhydramnios. There is still a significant risk of progression to renal failure pre or postnatally.

scholarly journals Correlation study between increased fetal movement during the third trimester and neonatal outcome

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Cuiqin Huang ◽  
Wei Han ◽  
Yajing Fan
Keyword(s):  
Amniotic Fluid ◽  
Pregnant Women ◽  
Gestational Age ◽  
Neonatal Outcome ◽  
Stress Test ◽  
Neonatal Outcomes ◽  
Control Group ◽  
Third Trimester ◽  
The Third ◽  
Fetal Movements

Abstract Background We aimed to analyze the correlation between increased fetal movements in the third trimester and neonatal outcomes. Methods We enrolled pregnant women (n = 219) who reported increased/excessive fetal movements in the third trimester in our hospital. A control group of healthy women (n = 278) who had undergone regular childbirth and delivery in our hospital during the same period and did not report abnormal fetal movements were also recruited. All pregnant women underwent fetal non-stress test. We analyzed the neonatal weight, appearance, pulse, grimace, activity, and respiration score, degrees of amniotic fluid contamination, amniotic fluid volume, conditions of umbilical cord around the neck and cord length, and incidence of small for gestational age. In addition, the incidence of preterm delivery, cesarean section rate, postpartum hemorrhage, and other postpartum complications were also analyzed. We then analyzed the correlation between increased/excessive fetal activity and neonatal outcomes. Results Women with complaints of increased/excessive fetal movements exhibited increased fetal movements mainly around 31 and 39 weeks of gestation. Several pregnancy variables, including number of previous delivery, gestational age (less than 34 weeks and more than 37 weeks) and vaginal birth rate, were associated with increased/excessive fetal movements. In addition, women who reported increased/excessive fetal movements had higher odds of large for gestational age (LGA), particularly those with gestational age over 37 weeks. Conclusion Increased/excessive fetal movements may be used to predict adverse neonatal outcome such as LGA.

Pharmacokinetics of ethanol and its metabolite, acetaldehyde, and fetolethality in the third-trimester pregnant guinea pig for oral administration of acute, multiple-dose ethanol

1986 ◽  
Vol 64 (8) ◽  
pp. 1060-1067 ◽  
Author(s):  
David W. Clarke ◽  
Nancy A. E. Steenaart ◽  
Christopher J. Slack ◽  
James F. Brien
Keyword(s):  
Guinea Pig ◽  
Amniotic Fluid ◽  
Ethanol Concentration ◽  
Fetal Brain ◽  
Maternal Blood ◽  
Time Interval ◽  
Gas Liquid Chromatography ◽  
Fetal Blood ◽  
Third Trimester ◽  
The Third

The pharmacokinetics of ethanol and its metabolite, acetaldehyde, were determined in the third-trimester pregnant guinea pig (56–59 days gestation) for oral intubation of four doses of 1 g ethanol/kg maternal body weight, administered at 1-h intervals. Animals (n = 4–7) were sacrificed at each of selected times during the 26-h study. Ethanol and acetaldehyde concentrations were determined by headspace gas-liquid chromatography. The maternal and fetal blood ethanol concentration–time curves were virtually superimposable, which indicated unimpeded bidirectional placental transfer of ethanol in the matemal–fetal unit. The blood and brain ethanol concentrations were similar in each of the maternal and fetal compartments during the study, which indicated rapid equilibrium distribution of ethanol. There was accumulation of ethanol in the amniotic fluid resulting in higher ethanol concentration compared with maternal and fetal blood during the elimination phase, which indicated that the amniotic fluid may serve as a reservoir for ethanol in utero. Acetaldehyde was measurable in all the biological fluids and tissues at concentrations that were at least 1000-fold less than the respective ethanol concentrations and were variable. There was ethanol-induced fetolethality that was delayed and variable among animals, and was 55% at 23 h. At this time interval, the ethanol concentrations in maternal blood and brain, fetal brain, and amniotic fluid were 35- to 53-fold greater and the acetaldehyde concentrations in maternal blood and fetal brain were four- to five-fold higher in the animals with dead fetuses compared with the guinea pigs with live litters. These data indicated that decreased ethanol elimination from the maternal–fetal unit was related temporally to the fetolethality.

Presence of CRH-binding protein in amniotic fluid and in umbilical cord plasma

1991 ◽  
Vol 125 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Toshihiro Suda ◽  
Mitsutoshi Iwashita ◽  
Takashi Sumitomo ◽  
Yoriko Nakano ◽  
Fumiko Tozawa ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Umbilical Cord ◽  
Peripheral Blood ◽  
Binding Protein ◽  
Normal Subjects ◽  
Normal Adult ◽  
Second Trimester ◽  
Third Trimester ◽  
The Third ◽  
Cord Plasma

Abstract. CRH-binding protein was present in the amniotic fluid and in the umbilical cord plasma after 15 weeks and 24 weeks of pregnancy, respectively. The size of the CRH-binding protein was similar to that in the peripheral blood from normal subjects. The level of the binding of CRH-binding protein in the umbilical cord plasma during the third trimester of pregnancy was also similar to that in the peripheral blood of neonates and normal adult subjects. The binding of CRH-binding protein was temporarily decreased at 40 weeks of pregnancy. These results indicate that fetal CRH-binding protein seems to be produced at least in the second trimester of pregnancy.

Amniotic Fluid α-Fetoprotein in Fetal Obstructive Uropathy

PEDIATRICS ◽  
1980 ◽  
Vol 66 (4) ◽  
pp. 537-539
Author(s):  
William M. Dean ◽  
Emory J. Bourdeau
Keyword(s):  
Amniotic Fluid ◽  
Congenital Anomalies ◽  
Obstructive Uropathy ◽  
In Utero ◽  
Posterior Urethral Valves ◽  
Urinary Obstruction ◽  
Type I ◽  
Urethral Valves

Amniotic fluid α-fetoprotein has been associated with various congenital anomalies. A diagnosis in utero of urinary obstruction associated with elevated α-fetoprotein is reported. Subsequent fulguration of type I posterior urethral valves was done. A possible mechanism for elevated α-fetoprotein with obstructive uropathy in utero is discussed.

Effects of Air Pollution in Amniotic Fluid Index in the Third Trimester of Pregnancy

2014 ◽  
Vol 2014 (1) ◽  
pp. 2626
Author(s):  
Mariana Azevedo Carvalho ◽  
Lisandra Stein Bernardes ◽  
karen Hettfleisch ◽  
Luciana D M Pastro ◽  
Silvia R.D.M Saldiva* ◽  
...  
Keyword(s):  
Air Pollution ◽  
Amniotic Fluid ◽  
Third Trimester ◽  
Amniotic Fluid Index ◽  
The Third
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