scholarly journals Co-inoculation of rhizobacteria promotes growth, yield, and nutrient contents in soybean and improves soil enzymes and nutrients under drought conditions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dilfuza Jabborova ◽  
Annapurna Kannepalli ◽  
Kakhramon Davranov ◽  
Abdujalil Narimanov ◽  
Yuriy Enakiev ◽  
...  

AbstractDrought stress is the major abiotic factor limiting crop production. Co-inoculating crops with nitrogen fixing bacteria and plant growth-promoting rhizobacteria (PGPR) improves plant growth and increases drought tolerance in arid or semiarid areas. Soybean is a major source of high-quality protein and oil for humans. It is susceptible to drought stress conditions. The co-inoculation of drought-stressed soybean with nodulating rhizobia and root-colonizing, PGPR improves the root and the shoot growth, formation of nodules, and nitrogen fixation capacity in soybean. The present study was aimed to observe if the co-inoculation of soybean (Glycine max L. (Merr.) nodulating with Bradyrhizobium japonicum USDA110 and PGPR Pseudomonas putida NUU8 can enhance drought tolerance, nodulation, plant growth, and nutrient uptake under drought conditions. The results of the study showed that co-inoculation with B. japonicum USDA110 and P. putida NUU8 gave more benefits in nodulation and growth of soybean compared to plants inoculated with B. japonicum USDA110 alone and uninoculated control. Under drought conditions, co-inoculation of B. japonicum USDA 110 and P. putida NUU8 significantly enhanced the root length by 56%, shoot length by 33%, root dry weight by 47%, shoot dry weight by 48%, and nodule number 17% compared to the control under drought-stressed. Co-inoculation with B. japonicum, USDA 110 and P. putida NUU8 significantly enhanced plant and soil nutrients and soil enzymes compared to control under normal and drought stress conditions. The synergistic use of B. japonicum USDA110 and P. putida NUU8 improves plant growth and nodulation of soybean under drought stress conditions. The results suggested that these strains could be used to formulate a consortium of biofertilizers for sustainable production of soybean under drought-stressed field conditions.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elsayed Mansour ◽  
Hany A. M. Mahgoub ◽  
Samir A. Mahgoub ◽  
El-Sayed E. A. El-Sobky ◽  
Mohamed I. Abdul-Hamid ◽  
...  

AbstractWater deficit has devastating impacts on legume production, particularly with the current abrupt climate changes in arid environments. The application of plant growth-promoting rhizobacteria (PGPR) is an effective approach for producing natural nitrogen and attenuating the detrimental effects of drought stress. This study investigated the influence of inoculation with the PGPR Rhizobium leguminosarum biovar viciae (USDA 2435) and Pseudomonas putida (RA MTCC5279) solely or in combination on the physio-biochemical and agronomic traits of five diverse Vicia faba cultivars under well-watered (100% crop evapotranspiration [ETc]), moderate drought (75% ETc), and severe drought (50% ETc) conditions in newly reclaimed poor-fertility sandy soil. Drought stress substantially reduced the expression of photosynthetic pigments and water relation parameters. In contrast, antioxidant enzyme activities and osmoprotectants were considerably increased in plants under drought stress compared with those in well-watered plants. These adverse effects of drought stress reduced crop water productivity (CWP) and seed yield‐related traits. However, the application of PGPR, particularly a consortium of both strains, improved these parameters and increased seed yield and CWP. The evaluated cultivars displayed varied tolerance to drought stress: Giza-843 and Giza-716 had the highest tolerance under well-watered and moderate drought conditions, whereas Giza-843 and Sakha-4 were more tolerant under severe drought conditions. Thus, co-inoculation of drought-tolerant cultivars with R. leguminosarum and P. putida enhanced their tolerance and increased their yield and CWP under water-deficit stress conditions. This study showed for the first time that the combined use of R. leguminosarum and P. putida is a promising and ecofriendly strategy for increasing drought tolerance in legume crops.


2020 ◽  
Vol 8 (9) ◽  
pp. 1329
Author(s):  
Zhiqiang Pang ◽  
Ying Zhao ◽  
Peng Xu ◽  
Diqiu Yu

Among abiotic stresses, drought is one of the most important factors limiting plant growth. To increase their drought tolerance and survival, most plants interact directly with a variety of microbes. Upland rice (Oryza sativa L.) is a rice ecotype that differs from irrigated ecotype rice; it is adapted to both drought-stress and aerobic conditions. However, its root microbial resources have not been explored. We isolated bacteria and fungi from roots of upland rice in Xishuangbanna, China. Four hundred sixty-two endophytic and rhizospheric isolates (337 bacteria and 125 fungi) were distributed. They were distributed among 43 genera on the basis of 16S rRNA and internal transcribed spacer (ITS) gene sequence analysis. Notably, these root microbes differed from irrigated rice root microbes in irrigated environments; for example, members of the Firmicutes phylum were enriched (by 28.54%) in the roots of the upland plants. The plant growth-promoting (PGP) potential of 217 isolates was investigated in vitro. The PGP ability of 17 endophytic and 10 rhizospheric isolates from upland rice roots was evaluated under well-irrigated and drought-stress conditions, and 9 fungal strains increased rice seedling shoot length, shoot and root fresh weight (FW), antioxidant capability, and proline (Pro) and soluble sugar contents. Our work suggests that fungi from upland rice roots can increase plant growth under irrigated and drought-stress conditions and can serve as effective microbial resources for sustainable agricultural production in arid regions.


AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohamed Ahmed Youssef ◽  
Ahmed Fathy Yousef ◽  
Muhammad Moaaz Ali ◽  
Alshaymaa I. Ahmed ◽  
Sobhi F. Lamlom ◽  
...  

AbstractThe effects of different fertilizers and biofertilizers on crop production to increase plant growth, improve quality and yield components (dry leaves yield, leaf protein, and stevioside) of crops has been extensively studied. However, the combination of both types of fertilizers have rarely been investigated. To explore the effect of different fertilizers and biofertilizers on stevia plant, a two-year field experiment was conducted to investigate the growth response of stevia plants under the influence of nitrogenous fertilizers (NFs) and effective microorganisms (EM). The experiment was laid out in a split-plot design, with EM as the main plot factor (−EM and +EM) and NFs as the subplot factor [control, chemical NFs (Ch-N) and organic NFs (Org-N)]. The results showed that, plants treated with EM and Org-N showed 2-, 2.2-, 2.4-, 2.5-, 3.3- and 3-fold increases in plant height, number of branches, total leaf area, plant fresh weight, plant dry weight and leaf dry yield, respectively, compared to untreated plants. Similarly, plants receiving EM along with Ch-N showed 1.86-, 1.7-, 2.2-, 2.12-, 3-, and 2.72-fold increases in the same traits. Total chlorophyll, protein, N, P, K and sativoside contents were increased by 88.8, 152, 138, 151.5, 43 and 137.5% when EM and Org-N were applied to stevia plants. Application of EM together with Ch-N increased these properties by 0.5, 127.7, 115, 216, 42.6 and 83.8%, respectively in the same traits. Overall, the combined application of NFs and EM improved growth, yield and nutrient accumulation in stevia plants.


Author(s):  
Dong Van Nguyen ◽  
Huong Mai Nguyen ◽  
Nga Thanh Le ◽  
Kien Huu Nguyen ◽  
Huong Mai Le ◽  
...  

ABSTRACTAbiotic stresses, including drought, detrimentally affect the growth and productivity of many economically important crop plants, leading to significant yield losses, which can result in food shortages and threaten the sustainability of agriculture. Balancing between plant growth and stress responses is one of the most important characters for agricultural application to maximize plant production. In this study, we initially report that copper nanoparticle priming positively regulates drought stress responses in maize. The copper nanoparticle priming plants displayed enhanced drought tolerance indicated by their higher leaf water content and plant biomass under drought as compared with water-treated plants. Moreover, our data showed that the treatment of copper nanoparticle on plants increased anthocyanin, chlorophyll and carotenoid contents compared to water-treated plants under drought stress conditions. Additionally, histochemical analyses with nitro blue tetrazolium and 3,3’-diaminobenzidine revealed that reactive oxygen species accumulation of priming plants was decreased as a result of enhancement of reactive oxygen species scavenging enzyme activities under drought. Furthermore, our comparative yield analysis data indicated applying copper nanoparticle to plant increased total seed number and grain yield under drought stress conditions. Our data provided the evidences that copper nanoparticle regulates plant protective mechanisms associated with drought tolerance, which is a promising approach for the production of drought tolerant crop plants.


2016 ◽  
Vol 40 (4) ◽  
pp. 537-550 ◽  
Author(s):  
JA Chowdhury ◽  
MA Karim ◽  
QA Khaliq ◽  
ARM Solaiman ◽  
JU Ahmed

A pot experiment was carried out in a venylhouse at Bangabandhu Sheikh Mujibur Rahman University during 2012 to investigate the growth, yield and yield contributing characters of ten selected soybean genotypes viz. Shohag, BARI Soybean-6, BARI Soybean-5, BD2331, BD2329, BD2336, BD 2340, BGM2093, G00015 and BGM2026 under drought stress and control conditions. Plant height, number of leaves, leaf area, shoot and root dry weight of all the genotypes were significantly affected by the stress. Among the genotypes Shohag, BARI Soybean-6 and BD2331 were found tolerant in relation to the growth under water stress conditions. The reduction in RGR values was more in the susceptible genotypes at the later stages of growth than in the tolerant genotypes. Seed yield of the genotypes was reduced from 42 to 68% due to drought (water) over non-stress. Susceptible genotypes showed greater reduction in seed yield than the tolerant genotypes.Bangladesh J. Agril. Res. 40(4): 537-550, December 2015


Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1534
Author(s):  
Chandra Mohan Singh ◽  
Poornima Singh ◽  
Chandrakant Tiwari ◽  
Shalini Purwar ◽  
Mukul Kumar ◽  
...  

Drought stress is considered a severe threat to crop production. It adversely affects the morpho-physiological, biochemical and molecular functions of the plants, especially in short duration crops like mungbean. In the past few decades, significant progress has been made towards enhancing climate resilience in legumes through classical and next-generation breeding coupled with omics approaches. Various defence mechanisms have been reported as key players in crop adaptation to drought stress. Many researchers have identified potential donors, QTLs/genes and candidate genes associated to drought tolerance-related traits. However, cloning and exploitation of these loci/gene(s) in breeding programmes are still limited. To bridge the gap between theoretical research and practical breeding, we need to reveal the omics-assisted genetic variations associated with drought tolerance in mungbean to tackle this stress. Furthermore, the use of wild relatives in breeding programmes for drought tolerance is also limited and needs to be focused. Even after six years of decoding the whole genome sequence of mungbean, the genome-wide characterization and expression of various gene families and transcriptional factors are still lacking. Due to the complex nature of drought tolerance, it also requires integrating high throughput multi-omics approaches to increase breeding efficiency and genomic selection for rapid genetic gains to develop drought-tolerant mungbean cultivars. This review highlights the impact of drought stress on mungbean and mitigation strategies for breeding high-yielding drought-tolerant mungbean varieties through classical and modern omics technologies.


Author(s):  
Dong Van Nguyen ◽  
Huong Mai Nguyen ◽  
Nga Thanh Le ◽  
Kien Huu Nguyen ◽  
Hoa Thi Nguyen ◽  
...  

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Mahmoud M. Gaballah ◽  
Azza M. Metwally ◽  
Milan Skalicky ◽  
Mohamed M. Hassan ◽  
Marian Brestic ◽  
...  

Drought is the most challenging abiotic stress for rice production in the world. Thus, developing new rice genotype tolerance to water scarcity is one of the best strategies to achieve and maximize high yield potential with water savings. The study aims to characterize 16 rice genotypes for grain and agronomic parameters under normal and drought stress conditions, and genetic differentiation, by determining specific DNA markers related to drought tolerance using Simple Sequence Repeats (SSR) markers and grouping cultivars, establishing their genetic relationship for different traits. The experiment was conducted under irrigated (normal) and water stress conditions. Mean squares due to genotype × environment interactions were highly significant for major traits. For the number of panicles/plants, the genotypes Giza179, IET1444, Hybrid1, and Hybrid2 showed the maximum mean values. The required sterility percentage values were produced by genotypes IET1444, Giza178, Hybrid2, and Giza179, while, Sakha101, Giza179, Hybrid1, and Hybrid2 achieved the highest values of grain yield/plant. The genotypes Giza178, Giza179, Hybrid1, and Hybrid2, produced maximum values for water use efficiency. The effective number of alleles per locus ranged from 1.20 alleles to 3.0 alleles with an average of 1.28 alleles, and the He values for all SSR markers used varied from 0.94 to 1.00 with an average of 0.98. The polymorphic information content (PIC) values for the SSR were varied from 0.83 to 0.99, with an average of 0.95 along with a highly significant correlation between PIC values and the number of amplified alleles detected per locus. The highest similarity coefficient between Giza181 and Giza182 (Indica type) was observed and are susceptible to drought stress. High similarity percentage between the genotypes (japonica type; Sakha104 with Sakha102 and Sakha106 (0.45), Sakha101 with Sakha102 and Sakha106 (0.40), Sakha105 with Hybrid1 (0.40), Hybrid1 with Giza178 (0.40) and GZ1368-S-5-4 with Giza181 (0.40)) was also observed, which are also susceptible to drought stress. All genotypes are grouped into two major clusters in the dendrogram at 66% similarity based on Jaccard’s similarity index. The first cluster (A) was divided into two minor groups A1 and A2, in which A1 had two groups A1-1 and A1-2, containing drought-tolerant genotypes like IET1444, GZ1386-S-5-4 and Hybrid1. On the other hand, the A1-2 cluster divided into A1-2-1 containing Hybrid2 genotype and A1-2-2 containing Giza179 and Giza178 at coefficient 0.91, showing moderate tolerance to drought stress. The genotypes GZ1368-S-5-4, IET1444, Giza 178, and Giza179, could be included as appropriate materials for developing a drought-tolerant variety breeding program. Genetic diversity to grow new rice cultivars that combine drought tolerance with high grain yields is essential to maintaining food security.


2018 ◽  
Vol 53 (4) ◽  
pp. 259-264
Author(s):  
MZ Hossain ◽  
Sushmita Dey ◽  
MS Islam

Groundwater arsenic contamination has become a threat to the crop production potential in the soils of vast areas of Bangladesh. Situation is grave in some districts of the country, particularly the southern part. A pot experiment was conducted to investigate the effects of arsenic treated irrigation water (0, 1, 2, 5 and 10 mgL-1), where a total of ten (10) irrigations were provided thus the treatments received 0, 10, 20, 50, and 100 mg arsenic (As) pot-1. Effects of applied levels of arsenic on Amaranthus gangeticus (Lal shak) were evaluated in terms of the growth, yield, major nutrients’ content, and their translocation in the plant. As treatments significantly reduced (p≤0.05) the dry weight of shoot and root by 19.31% and 44.03% respectively. Both total and available concentrations of nitrogen (N), potassium (K) and sulfur (S) were significantly (p≤ 0.05) suppressed by the As treatments, while only higher three doses significantly (p≤ 0.05) affected both levels of concentrations of phosphorus (P), calcium (Ca) and magnesium (Mg). Translocation coefficients for soil to root for P, K, S, and Mg were significantly reduced (p≤ 0.05), while translocation coefficients for root to shoot were significantly increased (p≤ 0.05) for K and S by 5 and 10 mgL-1 of arsenic treatments.Bangladesh J. Sci. Ind. Res.53(4), 259-264, 2018


2018 ◽  
Vol 19 (12) ◽  
pp. 4020 ◽  
Author(s):  
Xinbo Wang ◽  
Yanhua Xu ◽  
Jingjing Li ◽  
Yongzhe Ren ◽  
Zhiqiang Wang ◽  
...  

Drought is a major adversity that limits crop yields. Further exploration of wheat drought tolerance-related genes is critical for the genetic improvement of drought tolerance in this crop. Here, comparative proteomic analysis of two wheat varieties, XN979 and LA379, with contrasting drought tolerance was conducted to screen for drought tolerance-related proteins/genes. Virus-induced gene silencing (VIGS) technology was used to verify the functions of candidate proteins. A total of 335 differentially abundant proteins (DAPs) were exclusively identified in the drought-tolerant variety XN979. Most DAPs were mainly involved in photosynthesis, carbon fixation, glyoxylate and dicarboxylate metabolism, and several other pathways. Two DAPs (W5DYH0 and W5ERN8), dubbed TaDrSR1 and TaDrSR2, respectively, were selected for further functional analysis using VIGS. The relative electrolyte leakage rate and malonaldehyde content increased significantly, while the relative water content and proline content significantly decreased in the TaDrSR1- and TaDrSR2-knock-down plants compared to that in non-knocked-down plants under drought stress conditions. TaDrSR1- and TaDrSR2-knock-down plants exhibited more severe drooping and wilting phenotypes than non-knocked-down plants under drought stress conditions, suggesting that the former were more sensitive to drought stress. These results indicate that TaDrSR1 and TaDrSR2 potentially play vital roles in conferring drought tolerance in common wheat.


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