scholarly journals Microstructural, electrical and biological activity in $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2-\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_3$$ ceramic composites designed for tissue engineering applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Apurba Das ◽  
Pamu Dobbidi ◽  
Aman Bhardwaj ◽  
Varun Saxena ◽  
Lalit M. Pandey
Keyword(s):  
Protein Adsorption ◽  
Bovine Serum ◽  
Biomedical Applications ◽  
Electrostatic Interactions ◽  
Materials Science ◽  
Ceramic Composites ◽  
Biological Properties ◽  
Special Focus ◽  
Surface Charges ◽  
Fetal Bovine

AbstractThe article investigates electrically active ceramic composite of $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2$$ Ca 10 ( PO 4 ) 6 ( OH ) 2 (HAP) and $$\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_{3}$$ Ba 0.5 Sr 0.5 TiO 3 (BST) for biomedical applications. The study is a systematic blend of the materials science aspect of composites with a special focus on the dielectric and biological properties and their relationships. The article emphasized primarily extracting the dielectric constant ($$\epsilon _r)$$ ϵ r ) of the specimens (that lay in the range of 3–65) and related them to microstructural properties like the grain size and at.% of BST. A broad outlook on the importance of $$\epsilon _r$$ ϵ r in determining the suitability of bioceramics for clinical applications is presented. Bioactivity analysis of the specimens led to probing the surface charges (that were negative), and it was found crucial to the growth of dense apatite layers. Furthermore, the cytocompatibility of the specimens displayed cell viability above 100% for Day 1, which increased substantially for Day 3. To reveal other biological properties of the composites, protein adsorption studies using bovine serum albumin (BSA) and fetal bovine serum (FBS) was carried out. Electrostatic interactions govern the adsorption, and the mathematical dependence on surface charges is linear. The protein adsorption is also linearly correlated with the $$\epsilon _r$$ ϵ r , intrinsic to the biomaterials. We delve deeper into protein–biomaterials interactions by considering the evolution of the secondary structure of BSA adsorbed into the specimens. Based on the investigations, 20 at.% HAP–80 at.% BST (20H–80B) was established as a suitable composite comprising the desired features of HAP and BST. Such explorations of electrical and biological properties are interesting for modulating the behavior of bioceramic composites. The results project the suitability of 20H–80B for designing electrically active smart scaffolds for the proposed biomedical applications and are expected to incite further clinical trials.

Advances in nano-biomaterials and their applications in biomedicine

2021 ◽  
Author(s):  
Yogita Patil-Sen
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Biomedical Applications ◽  
Materials Science ◽  
Disease Diagnosis ◽  
Biological Properties ◽  
The Past ◽  
Physico Chemical ◽  
Wide Range ◽  
Biomolecules Detection

Nano0technology has received considerable attention and interest over the past few decades in the field of biomedicine due to the wide range of applications it provides in disease diagnosis, drug design and delivery, biomolecules detection, tissue engineering and regenerative medicine. Ultra-small size and large surface area of nanomaterials prove to be greatly advantageous for their biomedical applications. Moreover, the physico-chemical and thus, the biological properties of nanomaterials can be manipulated depending on the application. However, stability, efficacy and toxicity of nanoparticles remain challenge for researchers working in this area. This mini-review highlights the recent advances of various types of nanoparticles in biomedicine and will be of great value to researchers in the field of materials science, chemistry, biology and medicine.

scholarly journals Charged Gold Nanoparticles with Essentially Zero Serum Protein Adsorption in Undiluted Fetal Bovine Serum

2013 ◽  
Vol 135 (21) ◽  
pp. 7799-7802 ◽  
Author(s):  
Avinash K. Murthy ◽  
Robert J. Stover ◽  
William G. Hardin ◽  
Robert Schramm ◽  
Golay D. Nie ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Protein Adsorption ◽  
Serum Protein ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

Treatment of fetal bovine serum improves early development of porcine embryos by alleviating oxidative stress

2014 ◽  
Author(s):  
Seon-A Choi ◽  
Seong-Eun Mun ◽  
Pil-Soo Jeong ◽  
Hae-Jun Yang ◽  
Seung-Bin Yoon ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Early Development ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

МОДЕЛИРОВАНИЕ СИСТЕМ ЭКСТРАКОРПОРАЛЬНОГО СОЗРЕВАНИЯ ООЦИТОВ КРУПНОГО РОГАТОГО СКОТА С ИСПОЛЬЗОВАНИЕМ СТРУКТУРНЫХ КОМПОНЕНТОВ ОВАРИАЛЬНЫХ ФОЛЛИКУЛОВ *

2019 ◽  
pp. 20-22
Author(s):  
T.I. KUZMINA ◽  
I.V. CHISTYAKOVA
Keyword(s):  
Granulosa Cells ◽  
Follicular Fluid ◽  
Bovine Serum ◽  
Egg Quality ◽  
Fetal Bovine Serum ◽  
Reproductive Technologies ◽  
Blastocyst Stage ◽  
Preimplantation Embryos ◽  
Cultural Systems ◽  
Fetal Bovine

Создание эффективной унифицированной системы дозревания донорских ооцитов обеспечит повышение результативности инновационных клеточных репродуктивных технологий. В исследовании проведен сравнительный мониторинг показателеймейотического созревания ооцитов коров, созревших в различных системах, дополненных структурными компонентами фолликулов (СКФ стенки фолликулов, клетки гранулезы, белки) и фолликулярной жидкостью,а также потенций к развитию из них доимплантационных эмбрионов. Анализу подверглись ооциты, прокультивированные в следующих системах:среда ТС199 с добавлением 10 фетальной бычьей сыворотки (ФБС), 50 мкг/мл эстрадиола, 10 мкг/мл лютеинизирующего гормона (ЛГ), 10 мкг/мл фолликулостимулирующего гормона (ФСГ) среда ТС199 с 10 эстральной сывороткой коров среда ТС199 с 50 жидкости из фолликулов диаметром 9 мм среда ТС199 с добавлением белков фолликулярной жидкости молекулярной массой 65 кДасреда ТС199 с 10 ФБС и 1106 клеток гранулезы среда ТС199 с 10 ФБС и тканью фолликула. В культуральные среды ко всем исследованным группам ооцитов добавляли антибиотики. Использование CКФ обеспечило значительное снижение доли ооцитов с дегенерированным хроматином, что способствовало увеличению уровня доимпланационных эмбрионов на стадии бластоцисты. Так, доля бластоцист, развившихся из ооцитов, созревших в среде со стенками фолликулов,составила43,5. В этой же группе выявлен минимальный уровень дегенерированных зародышей (6,45). Полученные данные предлагается использовать при моделировании систем дозревания ооцитов коров с целью повышения качества яйцеклеток.The creation of an effective unified maturation system of donor oocytes provides an increase in the efficiency of innovative cellular reproductive technologies. The comparative analysis of the meiotic maturation indicators of bovine oocytes, which were matured in different cultural systems modified by follicular structural components (FSC follicular walls, granulosa cells, proteins) and follicular fluid, as well as the potential for preimplantation embryonic development were evaluated in this study. Oocytes matured in following cultural systems: medium TC199 supplemented with 10 fetal bovine serum and 50 g/ml of estradiol, 10 g/ml of luteinizing hormone (LH), 10 g/ml of folliclestimulating hormone (FSH) medium TC199 with 10 estrous cow serum medium TC199 with 50 liquid from follicles with a diameter of 9 mm medium TC199 supplemented with the follicular fluid proteins with molecular weight 65 kDa medium TC199 with 10 fetal bovine serum and 1106 granulosa cells medium TC199 with the addition of 10 fetal bovine serum and follicle tissues were analyzed. Antibiotics were added to cultural media of all experimental groups of oocytes. The usage of FSC ensured the decrease in the proportion of oocytes with degenerated chromatin, which contribute the rise of the level of preimplantation embryos at the blastocyst stage. Thus, the proportion of blastocysts developed from oocytes matured in medium supplemented with follicular walls was 43.5. In the same experimental group, the number of degenerated embryos was 6.45. The obtained data are supposed to be used for modeling the cultural systems of cow oocytes in order to improve the egg quality.

scholarly journals Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells

2020 ◽  
Vol 27 (2) ◽  
pp. 653-658
Author(s):  
Guan-Young Teo ◽  
Abdullah Rasedee ◽  
Nagi. A. AL-Haj ◽  
Chaw Yee Beh ◽  
Chee Wun How ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Bovine Serum ◽  
Breast Cancer Cells ◽  
Fetal Bovine Serum ◽  
Erythropoietin Receptor ◽  
Receptor Expression ◽  
Fetal Bovine

scholarly journals Prevalence of Bovine Viral Diarrhea Virus Genotypes and Antibody against those Viral Genotypes in Fetal Bovine Serum

1998 ◽  
Vol 10 (2) ◽  
pp. 135-139 ◽  
Author(s):  
Steven R. Bolin ◽  
Julia F. Ridpath
Keyword(s):  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Neutralizing Antibody ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Fetal Bovine ◽  
Viral Diarrhea Virus

One thousand lots of pooled fetal bovine serum (FBS) were tested for contamination with bovine viral diarrhea virus (BVDV) and/or for contamination with neutralizing antibody against BVDV. Noncytopathic or cytopathic BVDV was isolated from 203 lots of FBS. Analysis of the viral isolates identified 115 type 1 and 65 type 2 BVDV isolates. An additional 23 virus isolates were mixtures of >2 BVDV isolates and were not classified to viral genotype. Further characterization of the type 1 viruses identified 51 subgenotype 1a and 64 subgenotype 1b BVDV isolates. Viral neutralizing antibody was detected in 113 lots of FBS. Differential viral neutralization indicated that type 1 BVDV induced the antibody detected in 48 lots of FBS and type 2 BVDV induced the antibody detected in 16 lots of FBS.

scholarly journals Current Research on Polyelectrolyte Nanostructures: From Molecular Interactions to Biomedical Applications

Macromol ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 155-172
Author(s):  
Aristeidis Papagiannopoulos
Keyword(s):  
Physical Properties ◽  
Molecular Interactions ◽  
Interdisciplinary Research ◽  
Biomedical Applications ◽  
Electrostatic Interactions ◽  
Soft Matter ◽  
Extracellular Polysaccharides ◽  
Polymer Science ◽  
Biological Matter ◽  
Biomedical Field

Polyelectrolytes have been at the center of interdisciplinary research for many decades. In the field of polymer science and soft matter, they have provided the dimensions of electrostatic interactions, which opens a vast variety of opportunities for new physical properties and applications. In biological matter, polyelectrolytes are present in many forms, from extracellular polysaccharides to complex DNA molecules and proteins. This review discusses the recent research on polyelectrolytes covering the fundamental level of their conformations and nanostructures, their molecular interactions with materials that have close relevance to bioapplications and their applications in the biomedical field. This approach is motivated by the fact that the polyelectrolyte research is constantly active in all the aforementioned levels and continually affects many critical scientific areas.

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