scholarly journals Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
T. D. Jayasena Kaluarachchi ◽  
Paul M. Campbell ◽  
Rajitha Wickremasinghe ◽  
Shalindra Ranasinghe ◽  
Renu Wickremasinghe ◽  
...  

AbstractThe endemic strain of Leishmania donovani in Sri Lanka causes cutaneous leishmaniasis (CL) rather than more common visceral form. We have visualized biofilms and profiled the microbiome of lesions and unaffected skin in thirty-nine CL patients. Twenty-four lesions (61.5%) were biofilm-positive according to fluorescence in situ hybridization. Biopsies of biofilm-positive lesions were dominated by Pseudomonas, class Bacilli and Enterobacteriaceae and distinguished by significantly lower community evenness. Higher relative abundance of a class Bacilli OTU was detected in wound swabs versus contralateral skin. Wound swabs and biopsies had significantly distinct microbiome profiles and lower diversity compared to unaffected skin. Greater abundances of potentially pathogenic organisms were observed in wet ulcers, lesions with high parasite loads and large wounds. In summary, more than half of L. donovani associated CL wounds harboured biofilms and the wounds exhibited a distinct, less diverse, microbiome than unaffected skin.

2019 ◽  
Vol 113 (4) ◽  
pp. 180-190
Author(s):  
Thilini Dilhara Jayasena Kaluarachchi ◽  
Manjula Manoji Weerasekera ◽  
Andrew J. McBain ◽  
Shalindra Ranasinghe ◽  
Renu Wickremasinghe ◽  
...  

2009 ◽  
Vol 141 (4) ◽  
pp. 266
Author(s):  
N. Dinhopl ◽  
M.M. Mostegl ◽  
B. Richter ◽  
H. Weissenböck

Author(s):  
Thilini Jayasena Kaluarachchi ◽  
Rajitha Wickremasinghe ◽  
Manjula Weerasekera ◽  
Surangi Yasawardene ◽  
Andrew J McBain ◽  
...  

Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.


Author(s):  
Gary Bassell ◽  
Robert H. Singer

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.


2007 ◽  
Vol 177 (4S) ◽  
pp. 596-597
Author(s):  
Joseph P. Alukal ◽  
Bobby B. Najari ◽  
Wilson Chuang ◽  
Lata Murthy ◽  
Monica Lopez-Perdomo ◽  
...  

2006 ◽  
Vol 175 (4S) ◽  
pp. 287-288 ◽  
Author(s):  
Juliann M. Dziubinski ◽  
Michael F. Sarosdy ◽  
Paul R. Kahn ◽  
Mark D. Ziffer ◽  
William R. Love ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document