scholarly journals Evaluation of reference genes and characterization of the MYBs in xylem radial change of Chinese fir stem

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Kui-Peng Li ◽  
Wei Li ◽  
Gui-Yun Tao ◽  
Kai-Yong Huang
Keyword(s):  
Reference Genes ◽  
Southern China ◽  
Transcript Abundance ◽  
Chinese Fir ◽  
Heartwood Formation ◽  
Evergreen Species ◽  
Old Chinese ◽  
Timber Tree ◽  
Woody Tissues ◽  
Myb Proteins

AbstractThe radial change (RC) of tree stem is the process of heartwood formation involved in complex molecular mechanism. Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.), an evergreen species, is an important fast-growing timber tree in southern China. In this study, the top four stable genes (IDH, UBC2, RCA and H2B) were selected in RC tissues of 15 years old Chinese fir stem (RC15) and the genes (H2B, 18S, TIP41 and GAPDH) were selected in RC tissues of 30 years old Chinese fir stem (RC30). The stability of the reference genes is higher in RC30 than in RC15. Sixty-one MYB transcripts were obtained on the PacBio Sequel platform from woody tissues of one 30 years old Chinese fir stem. Based on the number of MYB DNA-binding domain and phylogenetic relationships, the ClMYB transcripts contained 21 transcripts of MYB-related proteins (1R-MYB), 39 transcripts of R2R3-MYB proteins (2R-MYB), one transcript of R1R2R3-MYB protein (3R-MYB) belonged to 18 function-annotated clades and two function-unknown clades. In RC woody tissues of 30 years old Chinese fir stem, ClMYB22 was the transcript with the greatest fold change detected by both RNA-seq and qRT-PCR. Reference genes selected in this study will be helpful for further verification of transcript abundance patterns during the heartwood formation of Chinese fir.

Effects of competition, age and climate on tree slenderness of Chinese fir plantations in southern China

2020 ◽  
Vol 458 ◽  
pp. 117815 ◽  
Author(s):  
Xiongqing Zhang ◽  
Hanchen Wang ◽  
Sophan Chhin ◽  
Jianguo Zhang
Keyword(s):  
Southern China ◽  
Chinese Fir

scholarly journals Projecting Stand Survival and Basal Area Based on a Self-Thinning Model for Chinese Fir Plantations

2020 ◽  
Vol 66 (3) ◽  
pp. 361-370
Author(s):  
Xiongqing Zhang ◽  
Quang V Cao ◽  
Hanchen Wang ◽  
Aiguo Duan ◽  
Jianguo Zhang
Keyword(s):  
Tree Species ◽  
Southern China ◽  
Critical Role ◽  
Basal Area ◽  
Chinese Fir ◽  
The Self ◽  
Survival Models ◽  
Stand Development ◽  
Growth And Survival ◽  
Stand Growth

Abstract The self-thinning rule has played a critical role in controlling stand stocking and modeling stand development in forest stands. Chinese fir (Cunninghamia lanceolata) is a native and fast-growing tree species used for timber production and is widely grown in southern China. Effective management of this important tree species requires accurate and reasonable predictions of stand growth and survival. Remeasured data from 48 plots distributed in Fujian, Jiangxi, Guangxi, and Sichuan provinces were used to develop models to predict stand survival and basal area based on the self-thinning trajectories. These trajectories were constructed using a self-thinning slope of –1.605, as suggested by Reineke (1933) (Method 1), and the slopes estimated either from two groups of sites (Method 2) or from climate variables (Method 3). Results indicated that the stand growth and survival models using Method 3 performed best, followed by Method 2 and Method 1. In addition, stand growth and survival curves predicted from Method 3 were more similar in shape to those from the observed values, as compared with Method 1. Overall, the models based on the self-thinning lines using climate-sensitive slopes provided reasonable predictions of the stand development dynamics. Therefore, these results facilitate modeling of the relation between stand growth/survival and self-thinning under climate change.

Carbon storage in a chronosequence of Chinese fir plantations in southern China

2013 ◽  
Vol 300 ◽  
pp. 68-76 ◽  
Author(s):  
Guang-Shui Chen ◽  
Zhi-Jie Yang ◽  
Ren Gao ◽  
Jin-Sheng Xie ◽  
Jian-Fen Guo ◽  
...  
Keyword(s):  
Carbon Storage ◽  
Southern China ◽  
Chinese Fir

scholarly journals Variations in biomass, nutrient contents and nutrient use efficiency among Chinese fir provenances

2011 ◽  
Vol 60 (1-6) ◽  
pp. 95-105 ◽  
Author(s):  
Pengfei Wu ◽  
Mulualem Tigabu ◽  
Xiangqing Ma ◽  
Per Christer Odén ◽  
Youlan He ◽  
...  
Keyword(s):  
Southern China ◽  
Nutrient Use Efficiency ◽  
Nutrient Content ◽  
Chinese Fir ◽  
Total Biomass ◽  
Improvement Program ◽  
Nutrient Contents ◽  
Nutrient Use ◽  
Use Efficiency ◽  
Phosphorus And Potassium

Abstract A provenance trial involving 16 Chinese fir provenances was established in southern China in 1979, and biomass, nutrient content and nutrient use efficiency were assessed at the age of 23. One-way analysis of variance revealed significant inter-provenance variation (p<0.0001) in measured variables. The mean total biomass ranged from 98.8±5.3 to 163.3±4.2 t ha−1, and the stemwood accounted for 47–65% of the total biomass, followed by roots (11–24%), stembark (7.4–13.7%), and needles and branches (< 10 %). Much of the nitrogen, phosphorus and potassium were stored in the needles, although the concentration varies among provenances. Whole-tree nutrient use efficiency varied from 115.4±2.1 to 180.2±1.02 g g−1, while efficiency in stemwood production ranged from 53.9±3.1 to 106.3±1.1 g g−1. Provenances did not display consistent variation in all measured variables, suggesting the need for multiple criteria for selection in future tree improvement program. In conclusion, the study reveals the existence of considerable variation in biomass production and nutrient use efficiency among Chinese fir provenances that can be exploited for selecting desirable genotypes for enhancing productivity of Chinese fir plantations.

scholarly journals Seedling emergence and early growth of Chinese fir under different light levels and seed positions: implications for natural regeneration

2018 ◽  
Vol 48 (9) ◽  
pp. 1034-1041 ◽  
Author(s):  
Bo Liu ◽  
Qingqing Liu ◽  
Stefani Daryanto ◽  
Xiangqing Ma ◽  
Si Guo ◽  
...  
Keyword(s):  
Natural Regeneration ◽  
Southern China ◽  
Seedling Emergence ◽  
Soil Surface ◽  
Light Level ◽  
Early Growth ◽  
Chinese Fir ◽  
Light Levels ◽  
Light Requirements ◽  
Moisture Conditions

Chinese fir, Cunninghamia lanceolata (Lamb.) Hook. (Taxodiaceae), is an evergreen conifer primarily distributed in southern China. This species exhibits very poor natural regeneration, possibly due to low light and a thick litter layer. To improve the understanding of the natural regeneration capacity of Chinese fir, in this study, we conducted a shade house experiment to determine the optimum light requirements and seed positions for seedling emergence and early growth. The experiment involved five light levels (100%, 60%, 40%, 15%, 5% of full sunlight) and four seed positions (1 cm beneath the soil surface without litter, on the soil surface without soil–seed contact, on the soil surface and covered with litter, and 1 cm beneath the soil surface and covered with litter). Seedling emergence was highest at 5%–15% sunlight, whereas seedling height, root length, root mass, stem mass, leaf mass, and total mass were highest at 60% sunlight. For each light level, seed position significantly affected emergence and growth. The above-litter position inhibited seedling emergence and survival, while the below-litter position favored seedling emergence and early growth, particularly under high light levels. Based on these results, to enhance natural regeneration of Chinese fir, we recommend periodical thinning to increase light into the understory after successful seedling emergence. We also recommend sowing seeds deeper into the litter to improve soil contact and moisture conditions.

scholarly journals Transcriptome analysis of immature xylem in Chinese fir at different developmental phases

2016 ◽  
Author(s):  
Yunxing Zhang ◽  
Xiaojiao Han ◽  
Jian Sang ◽  
Xuelian he ◽  
Mingying Liu ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Transcriptome Sequencing ◽  
Molecular Mechanisms ◽  
De Novo ◽  
Southern China ◽  
Wood Formation ◽  
Chinese Fir ◽  
Nac Transcription Factors ◽  
Transcriptome Variation ◽  
Developmental Phases

Background: Chinese fir [Cunninghamia lanceolata (Lamb .) Hook.], is one of the most important native tree species for timber production in southern China. An understanding of overall fast growing stage, stem growth stage and senescence stage cambium transcriptome variation is lacking. We used transcriptome sequencing to identify the repertoire of genes expressed during development of xylem tissue in Chinese fir, aiming to delineate the molecular mechanisms of wood formation. Results: We carried out transcriptome sequencing at three different cultivation ages (7Y, 15Y and 21Y) generating 68.71 million reads (13.88 Gbp). A total of 140,486 unigenes with a mean size of 568.64 base pairs (bp) were obtained via de novo assembly. Of these, 27,427 unigenes (19.52%) were further annotated by comparison to public protein databases. A total of 5,331 (3.79%) unigenes were mapped into 118 pathways by searching against the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG). Differentially expressed genes (DEG) analysis identified 3, 16 and 5,899 DEGs from the comparison of 7Y vs. 15Y, 7Y vs. 21Y and 15Y vs. 21Y, respectively, in the immature xylem tissues, including 2,638 significantly up-regulated and 3,280 significantly down-regulated genes. Besides, five NAC transcription factors, 190 MYB transcription factors, and 34 WRKY transcription factors were identified respectively from Chinese fir transcriptome. Conclusion: Our results revealed the active transcriptional pathways and identified the DEGs at different cultivation phases of Chinese fir wood formation. This transcriptome dataset will aid in understanding and carrying out future studies on the molecular basis of Chinese fir wood formation and contribute to future artificial production and applications.

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