scholarly journals Repeated exposure of the oral mucosa over 12 months with cold plasma is not carcinogenic in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
K. Evert ◽  
T. Kocher ◽  
A. Schindler ◽  
M. Müller ◽  
K. Müller ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Oral Mucosa ◽  
Atmospheric Pressure ◽  
Cold Plasma ◽  
Histological Analysis ◽  
Atmospheric Pressure Plasma ◽  
Non Invasive ◽  
Carcinogenic Effects ◽  
Cold Atmospheric Pressure Plasma

AbstractPeri-implantitis may result in the loss of dental implants. Cold atmospheric pressure plasma (CAP) was suggested to promote re-osseointegration, decrease antimicrobial burden, and support wound healing. However, the long-term risk assessment of CAP treatment in the oral cavity has not been addressed. Treatment with two different CAP devices was compared against UV radiation, carcinogen administration, and untreated conditions over 12 months. Histological analysis of 406 animals revealed that repeated CAP exposure did not foster non-invasive lesions or squamous cell carcinoma (SCCs). Carcinogen administration promoted non-invasive lesions and SCCs. Molecular analysis by a qPCR screening of 144 transcripts revealed distinct inflammatory profiles associated with each treatment regimen. Interestingly, CAP treatment of carcinogen-challenged mucosa did not promote but instead left unchanged or reduced the proportion of non-invasive lesions and SCC formation. In conclusion, repeated CAP exposure of murine oral mucosa was well tolerated, and carcinogenic effects did not occur, motivating CAP applications in patients for dental and implant treatments in the future.

scholarly journals Long-Term Risk Assessment for Medical Application of Cold Atmospheric Pressure Plasma

Diagnostics ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 210 ◽  
Author(s):  
Rico Rutkowski ◽  
Georg Daeschlein ◽  
Thomas von Woedtke ◽  
Ralf Smeets ◽  
Martin Gosau ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Plasma Medicine ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

Despite increasing knowledge gained based on multidisciplinary research, plasma medicine still raises various questions regarding specific effects as well as potential risks. With regard to significant statements about in vivo applicability that cannot be prognosticated exclusively based on in vitro data, there is still a deficit of clinical data. This study included a clinical follow-up of five probands who had participated five years previously in a study on the influence of cold atmospheric pressure plasma (CAP) on the wound healing of CO2 laser-induced skin lesions. The follow-up included a complex imaging diagnostic involving dermatoscopy, confocal laser scanning microscopy (CLSM) and hyperspectral imaging (HSI). Hyperspectral analysis showed no relevant microcirculatory differences between plasma-treated and non-plasma-treated areas. In summary of all the findings, no malignant changes, inflammatory reactions or pathological changes in cell architecture could be detected in the plasma-treated areas. These unique in vivo long-term data contribute to a further increase in knowledge about important safety aspects in regenerative plasma medicine. However, to confirm these findings and secure indication-specific dose recommendations, further clinical studies are required.

Scar formation of laser skin lesions after cold atmospheric pressure plasma (CAP) treatment: A clinical long term observation

2013 ◽  
Vol 1 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Hans-Robert Metelmann ◽  
Thi Thom Vu ◽  
Hoang Tung Do ◽  
Thi Nguyen Binh Le ◽  
Thi Ha Anh Hoang ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Skin Lesions ◽  
Scar Formation ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
Long Term Observation

scholarly journals Low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
A. Waskow ◽  
D. Butscher ◽  
G. Oberbossel ◽  
D. Klöti ◽  
P. Rudolf von Rohr ◽  
...  
Keyword(s):  
Electron Beam ◽  
Seed Germination ◽  
Atmospheric Pressure ◽  
Cold Plasma ◽  
Atmospheric Pressure Plasma ◽  
Low Energy ◽  
Germination Capacity ◽  
Electron Beam Treatment ◽  
Cold Atmospheric Pressure Plasma ◽  
Low Energy Electron

AbstractSprouts are germinated seeds that are often consumed due to their high nutritional content and health benefits. However, the conditions for germination strongly support the proliferation of present bacteria, including foodborne pathogens. Since sprouts are consumed raw or minimally processed, they are frequently linked to cases of food poisoning. Therefore, a seed decontamination method that provides efficient inactivation of microbial pathogens, while maintaining the germination capacity and quality of the seeds is in high demand. This study aimed to investigate and compare seed decontamination by cold atmospheric-pressure plasma and low-energy electron beam with respect to their impact on seed and seedling quality. The results show that both technologies provide great potential for inactivation of microorganisms on seeds, while cold plasma yielded a higher efficiency with 5 log units compared to a maximum of 3 log units after electron beam treatment. Both techniques accelerated seed germination, defined by the percentage of hypocotyl and leaf emergence at 3 days, with short plasma treatment (< 120 s) and all applied doses of electron beam treatment (8–60 kGy). However, even the lowest dose of electron beam treatment at 8 kGy in this study caused root abnormalities in seedlings, suggesting a detrimental effect on the seed tissue. Seeds treated with cold plasma had an eroded seed coat and increased seed wettability compared to electron beam treated seeds. However, these effects cannot explain the increase in the germination capacity of seeds as this was observed for both techniques. Future studies should focus on the investigation of the mechanisms causing accelerated seed germination and root abnormalities by characterizing the molecular and physiological impact of cold plasma and electron beam on seed tissue.

Fabrication and Performance of a Multidischarge Cold-Atmospheric Pressure Plasma Array

2021 ◽  
pp. 1-8
Author(s):  
Kenneth A. Cornell ◽  
Amanda White ◽  
Adam Croteau ◽  
Jessica Carlson ◽  
Zeke Kennedy ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
And Performance

scholarly journals Cold Atmospheric Pressure Plasma-Activated Medium Induces Selective Cell Death in Human Hepatocellular Carcinoma Cells Independently of Singlet Oxygen, Hydrogen Peroxide, Nitric Oxide and Nitrite/Nitrate

2021 ◽  
Vol 22 (11) ◽  
pp. 5548
Author(s):  
Yan Li ◽  
Tianyu Tang ◽  
Haejune Lee ◽  
Kiwon Song
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Singlet Oxygen ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Proliferative Effect ◽  
Huh7 Cells ◽  
Anti Cancer ◽  
Cold Atmospheric Pressure Plasma ◽  
Nitrite Nitrate

Cold atmospheric pressure plasma (CAP) and plasma-activated medium (PAM) induce cell death in diverse cancer cells and may function as powerful anti-cancer agents. The main components responsible for the selective anti-cancer effects of CAP and PAM remain elusive. CAP or PAM induces selective cell death in hepatocellular carcinoma cell lines Hep3B and Huh7 containing populations with cancer stem cell markers. Here, we investigated the major component(s) of CAP and PAM for mediating the selective anti-proliferative effect on Hep3B and Huh7 cells. The anti-proliferative effect of CAP was mediated through the medium; however, the reactive oxygen species scavenger N-acetyl cysteine did not suppress PAM-induced cell death. Neither high concentrations of nitrite or nitrite/nitrate nor a low concentration of H2O2 present in the PAM containing sodium pyruvate affected the viability of Hep3B and Huh7 cells. Inhibitors of singlet oxygen, superoxide anions, and nitric oxide retained the capacity of PAM to induce anti-cancer effects. The anti-cancer effect was largely blocked in the PAM prepared by placing an aluminum metal mesh, but not a dielectric PVC mesh, between the plasma source and the medium. Hence, singlet oxygen, hydrogen peroxide, nitric oxide, and nitrite/nitrate are not the main factors responsible for PAM-mediated selective death in Hep3B and Huh7 cells. Other factors, such as charged particles including various ions in CAP and PAM, may induce selective anti-cancer effects in certain cancer cells.

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