An online immobilized α-glucosidase microreactor for enzyme kinetics and inhibition assays

RSC Advances ◽  
2015 ◽  
Vol 5 (70) ◽  
pp. 56841-56847 ◽  
Author(s):  
Dong-Mei Liu ◽  
Juan Chen ◽  
Yan-Ping Shi
Keyword(s):  
Capillary Electrophoresis ◽  
Enzyme Kinetics ◽  
Inhibition Kinetics

A novel online α-glucosidase-immobilized microreactor was developed by immobilizing α-glucosidase on capillary inner wall. The microreactor combination with capillary electrophoresis was applied in studying enzyme kinetics and inhibition kinetics.

In vitro monitoring of GTPase activity and enzyme kinetics studies using capillary electrophoresis

2005 ◽  
Vol 383 (1) ◽  
pp. 92-97 ◽  
Author(s):  
Sandro Hillebrand ◽  
Wanius Garcia ◽  
Marcelo Delmar Cantú ◽  
Ana Paula Ulian de Araújo ◽  
Manami Tanaka ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Enzyme Kinetics ◽  
Gtpase Activity ◽  
Kinetics Studies

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

1968 ◽  
Vol 19 (03/04) ◽  
pp. 364-367 ◽  
Author(s):  
H. C Hemker ◽  
P. W Hemker
Keyword(s):  
Enzyme Kinetics ◽  
Blood Clotting ◽  
Competitive Inhibition ◽  
Competitive Inhibitor ◽  
Kinetics Of

SummaryThe enzyme kinetics of competitive inhibition under conditions prevailing in clotting tests are developed and a method is given to measure relative amounts of a competitive inhibitor by means of the t — D plot.

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

1965 ◽  
Vol 13 (01) ◽  
pp. 155-175 ◽  
Author(s):  
H. C Hemker ◽  
P.W Hemker ◽  
E. A Loeliger
Keyword(s):  
Kinetic Analysis ◽  
Enzyme Kinetics ◽  
Blood Clotting ◽  
Enzyme System ◽  
Enzyme Kinetic ◽  
Clotting Time ◽  
Standard Methods

SummaryApplication of the methods of enzyme-kinetic analysis to the results of clotting tests is feasible and can yield useful results. However, the standard methods of enzyme kinetics are not applicable without modifications imposed by the peculiarities of the blood-clotting enzyme system. The influence of the following complicating circumstances is calculated :1. Substrate is not present in excess.2. Only relative measures exist for concentrations of substrate or enzymes.3. Enzymes and substrates are often added together.4. Reagents are not pure.5. Clotting-time is our only measure for clotting-velocity.Formulas are deduced, which makes it possible to recognize the effect of these complications.

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