One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)

2017 ◽  
Vol 53 (80) ◽  
pp. 11040-11043 ◽  
Author(s):  
Yuanyuan Sun ◽  
Hui Tian ◽  
Chenghui Liu ◽  
Yueying Sun ◽  
Zhengping Li

A target-triggered loop-mediated isothermal amplification (TT-LAMP) mechanism is developed for simple one-step but highly sensitive detection of microRNAs.

RSC Advances ◽  
2021 ◽  
Vol 11 (39) ◽  
pp. 23975-23984
Author(s):  
Xue Yang ◽  
Yixia Ren ◽  
Hongmei Chai ◽  
Xiufang Hou ◽  
Zhixiang Wang ◽  
...  

Four fluorescent 2D Zn-MOFs based on a flexible triangular ligand and linear N-donor ligands are hydrothermally prepared and used to detect nitrobenzene in aqueous solution with high sensitivity, demonstrating their potential as fluorescent sensors.


2017 ◽  
Vol 9 (6) ◽  
pp. 1031-1037 ◽  
Author(s):  
Jingtao Liu ◽  
Yu Ding ◽  
Lifei Ji ◽  
Xin Zhang ◽  
Fengchun Yang ◽  
...  

Hexavalent chromium (Cr(vi)) is one of the most toxic heavy metal pollutants in groundwater, and thus the detection of Cr(vi) with high sensitivity, accuracy, and simplicity and low cost is of great importance.


RSC Advances ◽  
2020 ◽  
Vol 10 (67) ◽  
pp. 41110-41119
Author(s):  
Chaozhan Chen ◽  
Bin Ran ◽  
Zhenxing Wang ◽  
Hongli Zhao ◽  
Minbo Lan ◽  
...  

PDMS-based micropillar array electrodes with increased surface area and surface modification were developed to detect biomarkers with high sensitivity.


2015 ◽  
Vol 41 (04) ◽  
pp. 251-255
Author(s):  
Lih-Chiann Wang ◽  
Dean Huang

H7N9 avian influenza viruses have circulated in the human population and poultry flocks in China since 2013. H7N9 virus monitoring is imperative in Taiwan due to the frequent contact between China and Taiwan. Traditional viral molecular detection methods using RT-PCR and sequencing are time- and labor-intensive, thus a simpler and cheaper method with high sensitivity and specificity is worth developing. We successfully detected human and wild bird H7N9 viruses in this study using reverse transcription loop-mediated isothermal amplification (RT-LAMP) and oligonucleotide microarray. The detection limit was as low as one viral copy number. The specific matching reaction between the templates and microarray probes during hybridization ensured the high detection effectiveness. The excellent sensitivity and specificity of the RT-LAMP-microarray makes it a powerful H7N9 surveillance approach in Taiwan.


Sign in / Sign up

Export Citation Format

Share Document