Emerging investigator series: interacting effects of environmental factors on Daphnia magna removal of Escherichia coli from wastewater

2021 ◽  
Vol 7 (4) ◽  
pp. 726-738
Author(s):  
Seyyed M. H. Abtahi ◽  
Ojaswi Aryal ◽  
Niveen S. Ismail
Keyword(s):  
Escherichia Coli ◽  
Environmental Factors ◽  
Daphnia Magna ◽  
Environmental Conditions ◽  
E Coli

Zooplankton can significantly impact E. coli inactivation in wastewater, but inactivation rates are dependent on environmental conditions.

Disinfection performance of nanosilver and impacts of environmental conditions on viral inactivation by nanosilver

2013 ◽  
Vol 14 (1) ◽  
pp. 150-157 ◽  
Author(s):  
Yang Xu ◽  
Xiaona Chu ◽  
Jiangyong Hu ◽  
Say Leong Ong
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Humic Acid ◽  
Ionic Strength ◽  
Environmental Conditions ◽  
Contact Time ◽  
Chloride Ion ◽  
Viral Inactivation ◽  
E Coli ◽  
High Concentrations

Three types of nanosilver materials, which were commercial, chemically-synthesized and biologically-synthesized, respectively, were compared in terms of the disinfection efficiencies against Escherichia coli and MS2 coliphage in order to pinpoint promising material with the best performance. Disinfection results showed biologically-synthesized silver nanoparticles (referred to hereafter as ‘bio-AgNPs’) had the best disinfection performance, 10 mg/L of which was able to inactivate all the E. coli in 1 min (>6 log removals) and achieved 4 log removals of MS2 coliphage. Bio-AgNPs were therefore selected for further study in terms of effects of the concentration and contact time as well as the impacts of environmental conditions on the viral inactivation. Given the viral inactivation profile of bio-AgNPs shown in this study, it could be concluded that viral inactivation by bio-AgNPs could be inhibited by total organic carbon (TOC) (10 mg/L as humic acid) and chloride ion (5 mg/L) to a large extent while Ca2+/Mg2+/ionic strength only had minor effects on the viral inactivation at high concentrations (188 mg/L as CaCO3 of hardness or 5.6 mM of ionic strength, respectively). This part of the study may help enlighten further mechanism studies on viral inactivation by nanosilver.

scholarly journals The impact of anthropogenic and environmental factors on the variability of Escherichia coli in rivers in southwest Bangladesh

2021 ◽  
Author(s):  
M. M. Majedul Islam ◽  
Md. Atikul Islam
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Surface Water ◽  
Environmental Factors ◽  
Physicochemical Parameters ◽  
Sewage Treatment Plant ◽  
Oxygen Demand ◽  
Faecal Contamination ◽  
E Coli ◽  
The Impact

Abstract Faecal contamination of surface water sources is an important water quality issue worldwide. Although quite a few studies exist on surface water faecal contamination and variability of indicator bacteria, most of the studies have been based on larger river basins and in temperate region. The variability is relatively unknown in local scale and in tropical developing countries. In this study we assess how anthropogenic and environmental factors affect faecal contamination and physicochemical parameters in Rupsha and Bhairab rivers around Khulna city, Bangladesh. Water samples were collected from six locations of the rivers during a wet and dry period in 2018 to measure Escherichia coli (E. coli) concentrations. Water physicochemical parameters—temperature, turbidity, pH, dissolved oxygen, biochemical oxygen demand and chemical oxygen demand were also measured. Higher concentrations of E. coli were found in the sampling sites located near the densely populated urban area compared to the downstream site, which receives fewer amounts of discharges from sewer drains. All the E. coli samples violated bathing water quality standards. E. coli concentrations were found to be correlated positively with precipitation and turbidity. A linear regression model was applied, that explains large part of the variation in E. coli concentration (R2 = 0.42). Water quality index assessment was also ranked the water quality as ‘poor’ category; indicate that the water is unsuitable for uses in domestic and recreational purposes and high health risks involved with the water use. The study findings highlight the problem of untreated sewage discharge into the rivers. Implementation of sewage treatment plant with adequate capacity is highly recommended.

scholarly journals Kajian Budidaya Daphnia magna Menggunakan Air Rebusan Kedelai Dan Air Cucian Beras

PELAGICUS ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 45
Author(s):  
Hernika Simanjuntak ◽  
Ernik Yuliana ◽  
Sinar Pagi Sektiana
Keyword(s):  
Escherichia Coli ◽  
Population Growth ◽  
Daphnia Magna ◽  
Design Method ◽  
Chicken Manure ◽  
Natural Food ◽  
Feed Water ◽  
E Coli ◽  
Significant Difference ◽  
Boiled Water

ABSTRAKDaphnia magna memiliki banyak keunggulan sebagai pakan alami pada budidaya ikan fase larva. Tujuan penelitian ini adalah membandingkan pertumbuhan Daphnia magna pada budidaya dengan menggunakan beberapa sumber air pupuk/pakan. Penelitian ini dilakukan secara eksperimental dengan metode Rancangan Acak Lengkap (RAL) dengan perlakuan yaitu pemberian pupuk pakan: kotoran ayam, air cucian beras, dan air rebusan kedelai, masing-masing pada konsentrasi 2%, 5% dan 10% dengan 3 kali pengulangan. Analisis data yang digunakan adalah uji statistik ANOVA dan dilanjutkan dengan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa pertumbuhan populasi yang lebih baik adalah dengan penambahan pakan/pupuk air rebusan kedelai dengan konsentrasi berturut-turut 10%, 5%, dan 2%. Hasil uji ANOVA menunjukkan perbedaan yang siginifikan antar perlakuan, namun tidak berbeda signifikan pada penggunaan konsentrasi yang berbeda. Hasil uji BNT menunjukkan bahwa air rebusan kedelai konsentrasi 5% dan 10% memberikan respon yang lebih baik dengan rata-rata pertumbuhan populasi 346 ekor dan 534 ekor. Hasil pengujian kandungan protein dan lemak didapatkan hasil bahwa air rebusan kedelai lebih tinggi dibanding yang lainnya yaitu rata-rata sebesar 2,50% dan 5,77%. Pengujian kandungan Escherichia coli didapatkan hasil jika semua perlakuan menunjukkan kandungan E. coli yang negatif.ABSTRACTDaphnia magna has many advantages as natural food in larval stage fish culture. This study aims to compare the growth of Daphnia magna in cultivation using several sources of fertilizer/feed water. This research was conducted experimentally with a completely randomized design method with the treatment of feeding fertilizer: chicken manure, rice washing water, and soy boiled water, each at a concentration of 2%, 5% and 10% with 3 repetitions. The data analysis used was the ANOVA statistical test and continued with the least significant difference test (LSD). The results indicated that the better population growth was the addition of soybean boiled feed/water fertilizer with concentrations of 10%, 5%, and 2%, respectively. The results of the ANOVA test indicated that was a significant difference between treatments, but not significantly different at the use of different concentrations. LSD test results indicated that soybean boiled water with a concentration of 5% and 10% gave a better response with an average population growth of 346 and 534 individuals. The results of testing the protein and fat content showed that the cooking water for soybeans was higher than the others, namely 2.50% and 5.77%, respectively. The test for Escherichia coli content was obtained if all treatments showed negative E. coli content.

scholarly journals The effect of environmental factors on the abundance of cefotaxime-resistant Escherichia coli in Sunter River

2021 ◽  
Vol 909 (1) ◽  
pp. 012006
Author(s):  
Efadeswarni ◽  
F Y Amandita ◽  
N Puspandari ◽  
N Aini
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Environmental Factors ◽  
Pearson Correlation ◽  
Antibiotic Sensitivity ◽  
Environmental Parameters ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Antibiotic Sensitivity Test

Abstract The water quality of the Sunter River in Jakarta was classified as heavily polluted due to activities around the river, both domestic and non-domestic. As one of the environmental parameters for water quality, the presence of Escherichia coli (E. coli) is normally found any natural environment, and under certain conditions it can become resistant to antimicrobials due to genetic mutations. The mutated E. coli produces Extended Spectrum Beta-Lactamase (ESBL) enzymes and has a higher survival ability in antibiotic-contaminated river water, thus potentially endangering public health. This study was aimed to evaluate the effect of environmental factors on the abundance of ESBL producing E. coli and their resistance to antibiotic cefotaxime. Sampling was conducted in six locations representing the upstreams and the downstreams of Sunter River, following the Global Surveillance guidelines. E. coli strains were isolated using Tryptone Bile X-glucuronide (TBX) agar medium (with and without the addition of cefotaxime 4μg/ml) and the antibiotic sensitivity test of ESBL E. coli was conducted by performing a double-disk test. The results showed that the highest average abundance of ESBL E. coli was found in the sample taken from Sindang Station (904.24 x 104 colony per unit (CFU) / 100 mL) and the lowest was from Sunter Station (1,58 x 104 CFU / 100 mL). The results of the Bivariate Pearson correlation analysis showed that temperature, pH, and salinity were negatively correlated with the abundance of ESBL-producing E. coli bacteria.

Environmental Factors Affecting Escherichia coli Concentrations in Striped Venus Clam (Chamelea gallina L.) Harvested in the North Adriatic Sea

2017 ◽  
Vol 80 (9) ◽  
pp. 1429-1435 ◽  
Author(s):  
Giulia Tabanelli ◽  
Chiara Montanari ◽  
Aldo Gardini ◽  
Mirko Maffei ◽  
Chiara Prioli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Environmental Factors ◽  
Adriatic Sea ◽  
Microbiological Quality ◽  
Environmental Parameters ◽  
E Coli ◽  
The North ◽  
Chamelea Gallina ◽  
North Adriatic Sea

ABSTRACT The aim of this work was to evaluate the microbiological quality of striped venus clams (Chamelea gallina L.) harvested in the north Adriatic Sea during an 8-year monitoring period. A total of 387 samples were analyzed to assess the presence of Escherichia coli. Environmental parameters (salinity, pH, dissolved oxygen, seawater temperature, and freshwater outflow) were collected to find out a possible relationship between the E. coli counts and environmental factors. The results evidenced that the microbiological quality of the clams was good, with only about 5% of the samples not complying with European and Italian regulations for this product, that is, with E. coli counts higher than 230 most probable number (MPN) per 100 g of flesh and intravalvar liquid. Statistical analyses revealed a relationship between microbial contamination and the season and water temperature, probably due to the difference in the filtering activity of the mollusks. However, the main factor affecting the E. coli concentration in the clams turned out to be the flow rate of Marecchia, the major river that reaches the sea in the area of harvesting. In fact, a model fitted to evaluate the probability of finding a higher E. coli count in relation to the environmental parameters evidenced that it was an increase of the level of the Marecchia led to a higher probability of elevated E. coli contamination. This result could be explained by the higher supply of both nutrients and coliforms (including E. coli) when the river is higher and by the anthropogenic characteristics of the lands crossed by the river.

scholarly journals Synthesis, Characterisation and Cytotoxicity of Gold Microwires for Ultra-Sensitive Biosensor Development

2020 ◽  
Author(s):  
Nurul Akmal Che Lah ◽  
Robert Gray ◽  
Sonia Trigueros
Keyword(s):  
Escherichia Coli ◽  
Environmental Conditions ◽  
Biomarker Detection ◽  
Gram Negative ◽  
E Coli ◽  
Life Saving ◽  
Early Diagnostic

Abstract With the long-term goal of developing an ultra-sensitive microcantilever-based biosensor for versatile biomarker detection, new controlled bioreceptor-analytes systems are being explored to overcome the disadvantages of conventional ones. Gold (Au) microwires have been used as a probe to overcome the tolerance problem that occurs in response to changes in environmental conditions. However, the cytotoxicity of Au microwires is still unclear. Here, we examined the cytotoxicity of Au microwires systems using both commercial and as-synthesised Au microwires. In vitro experiments show that commercial Au microwires with an average quoted length of 5.6 µm are highly toxic against Gram-negative Escherichia coli (E. coli) at 50 µg/ml. However, this toxicity is due to the presence of CTAB surfactant not by the microwires. Conversely, the as-synthesised Au microwires show non-cytotoxicity even at the maximum viable concentration (330µg/ml). These findings may lead to the development of potentially life-saving cytotoxicity -free biosensors for an early diagnostic of potential diseases.

scholarly journals Unlocked capacity of proteins to attack membranes characteristic of aggregation: the evil for diseases and aging from Pandora’s box

2016 ◽  
Author(s):  
Liangzhong Lim ◽  
Yimei Lu ◽  
Jianxing Song
Keyword(s):  
Escherichia Coli ◽  
Environmental Factors ◽  
Neurodegenerative Diseases ◽  
Ribosomal Protein ◽  
Cellular Aging ◽  
Helical Conformation ◽  
E Coli ◽  
Specific Proteins ◽  
First Time ◽  
Plos Biol

AbstractAggregation of specific proteins is characteristic of a large spectrum of human diseases including all neurodegenerative diseases, while aggregation of non-specific proteins has been now identified to be a biomarker for cellular aging down to Escherichia coli. Previously, as facilitated with our discovery in 2005 that “completely insoluble” proteins could be all solubilized in unsalted water [Song (2009) FEBS Lett. 583: 953], we found that the TDP-43 prion-like domain in fact contains an intrinsic membrane-interacting subdomain [Lim et al. [2016] PLoS Biol. 14, e1002338]. We decrypted that ALS-causing mutations/cofactor-depletion act to render the wellstructured folds of cytosolic VAPB-MSP domain and SOD1 into highly disordered states, thus becoming buffer-insoluble. Most surprisingly, this also unlocks the amphiphilic/hydrophobic regions universally exiting in proteins, which thus acquire a novel capacity in abnormally interacting with membranes [Qin et al. (2013) F1000Res 2-221.v2; Lim (2016) BBA-Biomembranes. 1858: 2223]. Here we aimed extend our discovery to address two fundamental questions: 1) why many E. coli proteins become aggregated in aging; and 2) whether aggregation-prone proteins can also acquire a novel capacity in interacting with membranes; by dissecting the 557-residue S1 ribosomal protein into 7 fragments to disrupt its 6 S1 folds, followed by extensive CD and NMR characterizations. The results reveal that we have successfully eliminated all 6 S1 folds and fragment 4 becomes highly disordered and thus buffer-insoluble. Most strikingly, F4 does acquire a capacity in transforming into a helical conformation in membrane environments. Here, for the first time, our study deciphers that like ALScausing mutants, the disruption of a well-folded E. coli cytosolic protein also unlocks its amphiphilic/hydrophobic regions which are capable of abnormally interacting with membranes. Therefore, proteins, the most important functional players for all forms of life, can transform into membrane-toxic forms triggering diseases and aging, if their hydrophobic/amphiphilic regions are unlocked by genetic, pathological or/and environmental factors, which is characteristic of severe aggregation.

scholarly journals Synthesis, characterisation and cytotoxicity of gold microwires for ultra-sensitive biosensor development

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Nurul Akmal Che Lah ◽  
Robert Gray ◽  
Sonia Trigueros
Keyword(s):  
Escherichia Coli ◽  
Environmental Conditions ◽  
Biomarker Detection ◽  
In Vitro Experiments ◽  
Gram Negative ◽  
E Coli ◽  
Life Saving ◽  
Early Diagnostic

AbstractWith the long-term goal of developing an ultra-sensitive microcantilever-based biosensor for versatile biomarker detection, new controlled bioreceptor-analytes systems are being explored to overcome the disadvantages of conventional ones. Gold (Au) microwires have been used as a probe to overcome the tolerance problem that occurs in response to changes in environmental conditions. However, the cytotoxicity of Au microwires is still unclear. Here, we examined the cytotoxicity of Au microwires systems using both commercial and as-synthesised Au microwires. In vitro experiments show that commercial Au microwires with an average quoted length of 5.6 µm are highly toxic against Gram-negative Escherichia coli (E. coli) at 50 µg/mL. However, this toxicity is due to the presence of CTAB surfactant not by the microwires. Conversely, the as-synthesised Au microwires show non-cytotoxicity even at the maximum viable concentration (330 µg/mL). These findings may lead to the development of potentially life-saving cytotoxicity-free biosensors for an early diagnostic of potential diseases.

scholarly journals The Effect of Environmental Factors on Microbial Growth

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Rhezqy Furwati Jufri
Keyword(s):  
Escherichia Coli ◽  
Environmental Factors ◽  
Microbial Growth ◽  
Inhibitory Effect ◽  
Tetracycline Antibiotics ◽  
Experimental Procedure ◽  
E Coli ◽  
Chemical Factors ◽  
Strong Inhibitory Effect ◽  
Escherichia Coli Bacteria

Chemical factors that have inhibition based on the results of this practicum are Tetracycline and formalin. In this experiment, no type of liquid bath soap was found that inhibited the growth of E. coli. This can occur because of an error in the experimental procedure. Escherichia coli bacteria can grow optimally in the temperature range of 30 ° C. Formalin has a strong inhibitory effect on the growth of Escherichia coli bacteria, as well as Tetracycline antibiotics which can inhibit the growth of E. coli bacteria.

Factors affecting the occurrence of Escherichia coli O157 contamination in irrigation ponds on produce farms in the Suwannee River Watershed

2013 ◽  
Vol 59 (3) ◽  
pp. 175-182 ◽  
Author(s):  
Ganyu Gu ◽  
Zhiyao Luo ◽  
Juan M. Cevallos-Cevallos ◽  
Paige Adams ◽  
George Vellidis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Environmental Factors ◽  
Water Samples ◽  
Fecal Coliforms ◽  
Subsurface Water ◽  
Escherichia Coli O157 ◽  
Culturable Bacteria ◽  
River Watershed ◽  
E Coli ◽  
Suwannee River

Outbreaks of enteritis caused by Escherichia coli O157 associated with fresh produce have resulted in questions about the safety of irrigation water; however, associated risks have not been systematically evaluated. In this study, the occurrence and distribution of the human pathogen E. coli O157 from vegetable irrigation ponds within the Suwannee River Watershed in Georgia were investigated, and the relationship to environmental factors was analyzed. Surface and subsurface water samples were collected monthly from 10 vegetable irrigation ponds from March 2011 to February 2012. Escherichia coli O157 was isolated from enriched filtrates on CHROMagar and sorbitol MacConkey agar media and confirmed by an agglutination test. Presence of virulence genes stx1, stx2 , and eae was tested by polymerase chain reaction. In addition, 27 environmental variables of the sampled ponds were measured. Denaturing gradient gel electrophoresis was conducted for the analysis of bacterial communities in the water samples. Biserial correlation coefficients were calculated to evaluate the log10 colony-forming unit per millilitre correlations between the environmental factors and the occurrence of E. coli O157. Stepwise and canonical discriminant analyses were used to determine the factors that were associated with the presence and absence of E. coli O157 in water samples. All 10 ponds were positive for E. coli O157 some of the time, mainly in summer and fall of 2011. The temporal distribution of this bacterium differed among the 10 ponds. Temperature, rainfall, populations of fecal coliform, and culturable bacteria were positively correlated with the occurrence of E. coli O157 (P < 0.05), while the total nitrogen concentration, oxidation–reduction potential, and dissolved oxygen concentration were negatively correlated with the occurrence of this pathogen (P < 0.05). Temperature and rainfall were the most important factors contributing to the discrimination between samples with and without E. coli O157, followed by bacterial diversity and culturable bacteria population density. Bacterial numbers and diversity, including fecal coliforms and E. coli O157, increased after rainfall (and possibly runoff from pond margins) in periods with relatively high temperatures, suggesting that prevention of runoff may be important to minimize the risk of enteric pathogens in irrigation ponds.

Sign in / Sign up

Export Citation Format

Share Document