scholarly journals Intracellular green fluorescent protein–polyalanine aggregates are associated with cell death

2000 ◽  
Vol 348 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Julia RANKIN ◽  
Andreas WYTTENBACH ◽  
David C. RUBINSZTEIN
Keyword(s):  
Cell Death ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Cag Repeat ◽  
Cag Repeats ◽  
Spinocerebellar Ataxia Type ◽  
Aggregate Formation ◽  
Green Fluorescent ◽  
Intracellular Aggregates

Eight diseases, exemplified by Huntington's disease and spinocerebellar ataxia type 1, are caused by CAG-repeat expansion mutations. The CAG repeats are translated into expanded polyglutamine tracts, which are associated with deleterious novel functions. While these diseases are characterized by intraneuronal aggregate formation, it is unclear whether the aggregates cause disease. We have addressed this debate by generating intracellular aggregates with green fluorescent protein (GFP) fused to 19-37 alanines. No aggregates were seen in cells expressing native GFP or GFP fused to seven alanines. Aggregate-containing cells expressing GFP fused to 19-37 polyalanines show high rates of nuclear fragmentation compared with cells expressing the same constructs without aggregates, or cells expressing GFP fused to seven alanines. This suggests an association between aggregate formation and cell death.

Incorporation of green fluorescent protein into the essential envelope glycoprotein B of herpes simplex virus type 1

2002 ◽  
Vol 105 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Corinne Potel ◽  
Karin Kaelin ◽  
Isabelle Gautier ◽  
Pierre Lebon ◽  
Jacques Coppey ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Green Fluorescent Protein ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Envelope Glycoprotein ◽  
Fluorescent Protein ◽  
Green Fluorescent ◽  
Simplex Virus

scholarly journals Quantification of green fluorescent protein in cellular supernatant by capillary electrophoresis with laser-induced fluorescence detection for measurement of cell death

Talanta ◽  
2010 ◽  
Vol 81 (3) ◽  
pp. 948-953 ◽  
Author(s):  
Kristian E. Swearingen ◽  
Wendy P. Loomis ◽  
Benjamin Kehimkar ◽  
Brad T. Cookson ◽  
Norman J. Dovichi
Keyword(s):  
Cell Death ◽  
Capillary Electrophoresis ◽  
Green Fluorescent Protein ◽  
Fluorescence Detection ◽  
Fluorescent Protein ◽  
Laser Induced Fluorescence ◽  
Laser Induced Fluorescence Detection ◽  
Green Fluorescent

scholarly journals Replication-Competent Rhabdoviruses with Human Immunodeficiency Virus Type 1 Coats and Green Fluorescent Protein: Entry by a pH-Independent Pathway

1999 ◽  
Vol 73 (8) ◽  
pp. 6937-6945 ◽  
Author(s):  
Eli Boritz ◽  
Jennifer Gerlach ◽  
J. Erik Johnson ◽  
John K. Rose
Keyword(s):  
Human Immunodeficiency Virus ◽  
Green Fluorescent Protein ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Neutralizing Antibodies ◽  
Fluorescent Protein ◽  
Gene Encoding ◽  
Immunodeficiency Virus ◽  
Green Fluorescent

ABSTRACT We describe a replication-competent, recombinant vesicular stomatitis virus (VSV) in which the gene encoding the single transmembrane glycoprotein (G) was deleted and replaced by anenv-G hybrid gene encoding the extracellular and transmembrane domains of a human immunodeficiency virus type 1 (HIV-1) envelope protein fused to the cytoplasmic domain of VSV G. An additional gene encoding a green fluorescent protein was added to permit rapid detection of infection. This novel surrogate virus infected and propagated on cells expressing the HIV receptor CD4 and coreceptor CXCR4. Infection was blocked by SDF-1, the ligand for CXCR4, by antibody to CD4 and by HIV-neutralizing antibody. This virus, unlike VSV, entered cells by a pH-independent pathway and thus supports a pH-independent pathway of HIV entry. Additional recombinants carrying hybrid env-G genes derived from R5 or X4R5 HIV strains also showed the coreceptor specificities of the HIV strains from which they were derived. These surrogate viruses provide a simple and rapid assay for HIV-neutralizing antibodies as well as a rapid screen for molecules that would interfere with any stage of HIV binding or entry. The viruses might also be useful as HIV vaccines. Our results suggest wide applications of other surrogate viruses based on VSV.

High-Throughput Technology: Green Fluorescent Protein to Monitor Cell Death

2005 ◽  
pp. 121-138 ◽  
Author(s):  
Marylène Fortin ◽  
Ann-Muriel Steff ◽  
Patrice Hugo
Keyword(s):  
Cell Death ◽  
Green Fluorescent Protein ◽  
High Throughput ◽  
Fluorescent Protein ◽  
High Throughput Technology ◽  
Green Fluorescent

scholarly journals Intracellular green fluorescent protein‒polyalanine aggregates are associated with cell death

2000 ◽  
Vol 348 (1) ◽  
pp. 15 ◽  
Author(s):  
Julia RANKIN ◽  
Andreas WYTTENBACH ◽  
David C. RUBINSZTEIN
Keyword(s):  
Cell Death ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

scholarly journals Protective Role of Autophagy in Palmitate-Induced INS-1 β-Cell Death

Endocrinology ◽  
2008 ◽  
Vol 150 (1) ◽  
pp. 126-134 ◽  
Author(s):  
Sung-E Choi ◽  
Sung-Mi Lee ◽  
Youn-Jung Lee ◽  
Ling-Ji Li ◽  
Soo-Jin Lee ◽  
...  
Keyword(s):  
Cell Death ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Mammalian Target Of Rapamycin ◽  
Protective Role ◽  
Target Of Rapamycin ◽  
Β Cells ◽  
Β Cell ◽  
Green Fluorescent

Autophagy, a vacuolar degradative pathway, constitutes a stress adaptation that avoids cell death or elicits the alternative cell-death pathway. This study was undertaken to determine whether autophagy is activated in palmitate (PA)-treated β-cells and, if activated, what the role of autophagy is in the PA-induced β-cell death. The enhanced formation of autophagosomes and autolysosomes was observed by exposure of INS-1 β-cells to 400 μm PA in the presence of 25 mm glucose for 12 h. The formation of green fluorescent protein-LC3-labeled structures (green fluorescent protein-LC3 dots), with the conversion from LC3-I to LC3-II, was also distinct in the PA-treated cells. The phospho-mammalian target of rapamycin level, a typical signal pathway that inhibits activation of autophagy, was gradually decreased by PA treatment. Blockage of the mammalian target of rapamycin signaling pathway by treatment with rapamycin augmented the formation of autophagosomes but reduced PA-induced INS-1 cell death. In contrast, reduction of autophagosome formation by knocking down the ATG5, inhibition of fusion between autophagosome and lysosome by treatment with bafilomycin A1, or inhibition of proteolytic degradation by treatment with E64d/pepstatin A, significantly augmented PA-induced INS-1 cell death. These findings showed that the autophagy system could be activated in PA-treated INS-1 β-cells, and suggested that the induction of autophagy might play an adaptive and protective role in PA-induced cell death. Autophagy is activated in palmitate-treated insulinoma-1 beta cells, and the induction of autophagy plays a protective role in palmitate-induced beta cell death.

scholarly journals Application of green fluorescent protein to measure antimicrobial efficacy and the kinetics of cell death against Escherichia coli

2017 ◽  
Vol 141 ◽  
pp. 67-72
Author(s):  
Richard Greenhalgh ◽  
Malcolm Greenhalgh ◽  
Fadwa Alshareef ◽  
Geoffrey D. Robson
Keyword(s):  
Escherichia Coli ◽  
Cell Death ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Antimicrobial Efficacy ◽  
Green Fluorescent ◽  
Kinetics Of
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