Overexpression of cysteine proteinase 2 in Entamoeba histolytica or Entamoeba dispar increases amoeba-induced monolayer destruction in vitro but does not augment amoebic liver abscess formation in gerbils

2001 ◽  
Vol 3 (1) ◽  
pp. 13-20 ◽  
Author(s):  
A. Hellberg ◽  
R. Nickel ◽  
H. Lotter ◽  
E. Tannich ◽  
I. Bruchhaus
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Cysteine Proteinase ◽  
Abscess Formation ◽  
Amoebic Liver Abscess ◽  
Entamoeba Dispar

Entamoeba histolytica: induction of cyclooxygenase-2 expression during amoebic liver abscess formation in hamsters (Mesocricetus auratus)

2004 ◽  
Vol 106 (3-4) ◽  
pp. 119-125 ◽  
Author(s):  
B. Sánchez-Ramı́rez ◽  
M. Ramı́rez-Gil ◽  
I. Vázquez-Moctezuma ◽  
E. Ramos-Martı́nez ◽  
P. Talamás-Rohana
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Mesocricetus Auratus ◽  
Cyclooxygenase 2 ◽  
Abscess Formation ◽  
Amoebic Liver Abscess

Entamoeba histolytica: Inflammatory process during amoebic liver abscess formation involves cyclooxygenase-2 expression in macrophages and trophozoites

2006 ◽  
Vol 114 (3) ◽  
pp. 154-159 ◽  
Author(s):  
A. Gutiérrez-Alarcón ◽  
M. Moguel-Torres ◽  
O. Mata-Leyva ◽  
G. Cuellar-Nevárez ◽  
T. Siqueiros-Cendón ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Inflammatory Process ◽  
Cyclooxygenase 2 ◽  
Abscess Formation ◽  
Amoebic Liver Abscess

scholarly journals Entamoeba histolytica acetyl–CoA synthetase: biomarker of acute amoebic liver abscess

2014 ◽  
Vol 4 (6) ◽  
pp. 446-450
Author(s):  
Lim Boon Huat ◽  
Alfonso Olivos Garcia ◽  
Tan Zi Ning ◽  
Wong Weng Kin ◽  
Rahmah Noordin ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Amoebic Liver Abscess ◽  
Acetyl Coa

Linearolactone and Kaempferol Disrupt the Actin Cytoskeleton in Entamoeba histolytica: Inhibition of Amoebic Liver Abscess Development

2020 ◽  
Vol 83 (12) ◽  
pp. 3671-3680
Author(s):  
José Antonio Velázquez-Domínguez ◽  
Verónica Ivonne Hernández-Ramírez ◽  
Fernando Calzada ◽  
Luis Varela-Rodríguez ◽  
Diana L. Pichardo-Hernández ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Amoebic Liver Abscess

scholarly journals CpG-Oligodeoxynucleotide Is a Potent Adjuvant with an Entamoeba histolytica Gal-Inhibitable Lectin Vaccine against Amoebic Liver Abscess in Gerbils

2006 ◽  
Vol 74 (1) ◽  
pp. 528-536 ◽  
Author(s):  
Catherine P. A. Ivory ◽  
Kathy Keller ◽  
Kris Chadee
Keyword(s):  
Entamoeba Histolytica ◽  
Protozoan Parasite ◽  
Amoebic Liver Abscess ◽  
Interleukin 12 ◽  
Protective Effects ◽  
Cpg Odn ◽  
Cpg Oligodeoxynucleotide ◽  
Th1 Cytokines ◽  
And Control

ABSTRACT The protozoan parasite Entamoeba histolytica causes invasive amoebiasis characterized by amoebic dysentery and liver abscesses (ALA). The E. histolytica galactose/N-acetyl-d-galactosamine-inhibitable lectin (Gal-lectin), an immunogenic surface molecule involved in colonization and invasion, is a promising vaccine candidate against amoebiasis. Gal-lectin is known to induce Th1 cytokines in macrophages and spleen cells in vitro, and a Th1 response is thought to be protective against ALA. In this study, we report the use of cytosine guanine oligodeoxynucleotide (CpG-ODN) as adjuvant to augment Th1 responses against Gal-lectin in the gerbil model of ALA. Gerbils were vaccinated intramuscularly with the native Gal-lectin plus CpG-ODN or a paired non-CpG control GpC-ODN, and control gerbils received CpG-ODN alone. One week after the last boost gerbils were challenged intrahepatically with 106 amoebae. Gerbils receiving CpG-ODN as adjuvant with Gal-lectin were completely protected against the development of ALA, whereas 50% of gerbils receiving GpC-ODN and Gal-lectin developed ALA and 85% of controls developed ALA. Stronger lymphoproliferation in response to the Gal-lectin and higher prechallenge titers of serum Gal-lectin-specific antibodies, capable of blocking amoebic adherence, were observed when CpG-ODN was used as adjuvant. Gerbils vaccinated with CpG-ODN and Gal-lectin also had significantly higher levels of gamma interferon, interleukin-12 (IL-12), and IL-2 mRNA than controls. These data indicate that CpG-ODN can enhance the Th1 responses, which improve the protective effects of Gal-lectin. This is the first report of the use of CpG as a potent Th1 adjuvant with Gal-lectin to increase protection against ALA formation.

Entamoeba histolytica antigenic protein detected in pus aspirates from patients with amoebic liver abscess

2013 ◽  
Vol 134 (4) ◽  
pp. 504-510 ◽  
Author(s):  
Nurulhasanah Othman ◽  
Zeehaida Mohamed ◽  
Maya Mazuwin Yahya ◽  
Voon Meng Leow ◽  
Boon Huat Lim ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Amoebic Liver Abscess ◽  
Antigenic Protein

scholarly journals Protection against Invasive Amebiasis by a Single Monoclonal Antibody Directed against a Lipophosphoglycan Antigen Localized on the Surface of Entamoeba histolytica

1997 ◽  
Vol 186 (9) ◽  
pp. 1557-1565 ◽  
Author(s):  
Alexandra Marinets ◽  
Tonghai Zhang ◽  
Nancy Guillén ◽  
Pierre Gounon ◽  
Barbara Bohle ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Passive Immunization ◽  
Outer Face ◽  
Protective Capacity ◽  
Con A ◽  
Entamoeba Dispar ◽  
Confocal Immunofluorescence ◽  
Scid Mouse Model ◽  
First Time

A panel of monoclonal antibodies was raised from mice immunized with a membrane preparation from Entamoeba histolytica, the pathogenic species causing invasive amebiasis in humans. Antibody EH5 gave a polydisperse band in immunoblots from membrane preparations from different E. histolytica strains, and a much weaker signal from two strains of the nonpathogenic species Entamoeba dispar. Although the exact chemical structure of the EH5 antigen is not yet known, the ability of the antigen to be metabolically radiolabeled with [32P]phosphate or [3H]glucose, its sensitivity to digestion by mild acid and phosphatidylinositol-specific phospholipase C, and its specific extraction from E. histolytica trophozoites by a method used to prepare lipophosphoglycans from Leishmania showed that it could be classified as an amebal lipophosphoglycan. Confocal immunofluorescence and immunogold labeling of trophozoites localized the antigen on the outer face of the plasma membrane and on the inner face of internal vesicle membranes. Antibody EH5 strongly agglutinated amebas in a similar way to concanavalin A (Con A), and Con A bound to immunoaffinity-purified EH5 antigen. Therefore, surface lipophosphoglycans may play an important role in the preferential agglutination of pathogenic amebas by Con A. The protective ability of antibody EH5 was tested in a passive immunization experiment in a severe combined immunodeficient (SCID) mouse model. Intrahepatic challenge of animals after administration of an isotype-matched control antibody or without treatment led to the development of a liver abscess in all cases, whereas 11 out of 12 animals immunized with the EH5 antibody developed no liver abscess. Our results demonstrate the importance and, for the first time, the protective capacity of glycan antigens on the surface of the amebas.

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