O-28: Characterization of IRS-1 molecules containing single SH2-protein binding determinants

2009 ◽  
Vol 104 (S 02) ◽  
pp. 37-38
Author(s):  
Lynne Yenush ◽  
Martin G. Myers ◽  
Timothy C. Grammer ◽  
John Blenis ◽  
Morris F. White
Keyword(s):  
Protein Binding ◽  
Binding Determinants

scholarly journals Ultrafiltration Method for Plasma Protein Binding Studies and Its Limitations

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 382
Author(s):  
Camelia-Maria Toma ◽  
Silvia Imre ◽  
Camil-Eugen Vari ◽  
Daniela-Lucia Muntean ◽  
Amelia Tero-Vescan
Keyword(s):  
Protein Binding ◽  
Plasma Protein Binding ◽  
Plasma Protein ◽  
Specific Binding ◽  
Critical Role ◽  
Volume Ratio ◽  
Binding Studies ◽  
Experimental Conditions ◽  
Key Aspects

Plasma protein binding plays a critical role in drug therapy, being a key part in the characterization of any compound. Among other methods, this process is largely studied by ultrafiltration based on its advantages. However, the method also has some limitations that could negatively influence the experimental results. The aim of this study was to underline key aspects regarding the limitations of the ultrafiltration method, and the potential ways to overcome them. The main limitations are given by the non-specific binding of the substances, the effect of the volume ratio obtained, and the need of a rigorous control of the experimental conditions, especially pH and temperature. This review presents a variety of methods that can hypothetically reduce the limitations, and concludes that ultrafiltration remains a reliable method for the study of protein binding. However, the methodology of the study should be carefully chosen.

scholarly journals Characterization of the adenosine deaminase-adenosine deaminase complexing protein binding reaction.

1990 ◽  
Vol 265 (31) ◽  
pp. 19312-19318
Author(s):  
W.P. Schrader ◽  
C.A. West ◽  
A.D. Miczek ◽  
E.K. Norton
Keyword(s):  
Protein Binding ◽  
Adenosine Deaminase ◽  
Binding Reaction

scholarly journals Interaction cloning and characterization of RoBPI, a novel protein binding to human Ro ribonucleoproteins

RNA ◽  
2000 ◽  
Vol 6 (1) ◽  
pp. 66-78 ◽  
Author(s):  
PASCAL BOUFFARD ◽  
ELIE BARBAR ◽  
FRANCIS BRIÈRE ◽  
GILLES BOIRE
Keyword(s):  
Protein Binding ◽  
Novel Protein

Characterization of high mobility group protein binding to cisplatin-damaged DNA

1992 ◽  
Vol 188 (3) ◽  
pp. 1286-1294 ◽  
Author(s):  
Paul C. Billings ◽  
Richard J. Davis ◽  
Beatrice N. Engelsberg ◽  
Kirsten A. Skov ◽  
Edward N. Hughes
Keyword(s):  
Protein Binding ◽  
High Mobility ◽  
High Mobility Group ◽  
High Mobility Group Protein ◽  
Group Protein ◽  
Damaged Dna

scholarly journals Effect of Protein Binding on Ultrafast DNA Dynamics: Characterization of a DNA:APE1 Complex

2005 ◽  
Vol 89 (6) ◽  
pp. 4129-4138 ◽  
Author(s):  
Sobhan Sen ◽  
Nicole A. Paraggio ◽  
Latha A. Gearheart ◽  
Ellen E. Connor ◽  
Ala Issa ◽  
...  
Keyword(s):  
Protein Binding ◽  
Dna Dynamics

Abstract 1480: Characterization of C35, a novel protein binding partner of p73, in gynaecological cancers

2010 ◽  
Author(s):  
Sanna Ching-shan Wong ◽  
Thomas Ho-yin Leung ◽  
Karen Kar-loen Chan ◽  
Hextan Yuen-sheung Ngan
Keyword(s):  
Protein Binding ◽  
Binding Partner ◽  
Novel Protein

scholarly journals Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids.

1992 ◽  
Vol 4 (4) ◽  
pp. 397-411 ◽  
Author(s):  
V Citovsky ◽  
M L Wong ◽  
A L Shaw ◽  
B V Prasad ◽  
P Zambryski
Keyword(s):  
Nucleic Acids ◽  
Protein Binding ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Virus Movement

scholarly journals Isolation and Characterization of the DNA and Protein Binding Activities of Adenovirus Core Protein V

2014 ◽  
Vol 88 (16) ◽  
pp. 9287-9296 ◽  
Author(s):  
J. Perez-Vargas ◽  
R. C. Vaughan ◽  
C. Houser ◽  
K. M. Hastie ◽  
C. C. Kao ◽  
...  
Keyword(s):  
Protein Binding ◽  
Core Protein ◽  
Isolation And Characterization
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