Flavonoid profiling by LC-MS IN 14 – 3-3ν knockout mutants of Arabidopsis thaliana during drought stress conditions

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
F Nabbie ◽  
O Shperdheja ◽  
J Millot ◽  
J Lindberg ◽  
B Peethambaran
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Conditions ◽  
Knockout Mutants

scholarly journals Alterations of Lysine Modifications on the Histone H3 N-Tail under Drought Stress Conditions in Arabidopsis thaliana

2008 ◽  
Vol 49 (10) ◽  
pp. 1580-1588 ◽  
Author(s):  
Jong-Myong Kim ◽  
Taiko Kim To ◽  
Junko Ishida ◽  
Taeko Morosawa ◽  
Makiko Kawashima ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Histone H3 ◽  
Stress Conditions

The role of SORBITOL DEHYDROGENASE in Arabidopsis thaliana

2012 ◽  
Vol 39 (6) ◽  
pp. 462 ◽  
Author(s):  
Marta Nosarzewski ◽  
A. Bruce Downie ◽  
Benhong Wu ◽  
Douglas D. Archbold
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Sorbitol Dehydrogenase ◽  
Primary Role ◽  
Dependent Manner ◽  
Plant Leaves ◽  
Wild Type ◽  
Concentration Dependent Manner ◽  
Knockout Mutants

SORBITOL DEHYDROGENASE (SDH, EC 1.1.1.14) catalyses the interconversion of polyols and ketoses (e.g. sorbitol ↔ fructose). Using two independent Arabidopsis thaliana (L.) Heynh. sdh knockout mutants, we show that SDH (At5g51970) plays a primary role in sorbitol metabolism as well as an unexpected role in ribitol metabolism. Sorbitol content increased in both wild-type (WT) and mutant plant leaves during drought stress, but mutants showed a dramatically different phenotype, dying even if rewatered. The lack of functional SDH in mutant plants was accompanied by accumulation of foliar sorbitol and at least 10-fold more ribitol, neither of which decreased in mutant plants after rewatering. In addition, mutant plants were uniquely sensitive to ribitol in a concentration-dependent manner, which either prevented them from completing seed germination or inhibited seedling development, effects not observed with other polyols or with ribitol-treated WT plants. Ribitol catabolism may occur solely through SDH in A. thaliana, though at only 30% the rate of that for sorbitol. The results indicate a role for SDH in metabolism of sorbitol to fructose and in ribitol conversion to ribulose in A. thaliana during recovery from drought stress.

scholarly journals Free prolin content in Arabidopsis thaliana Cat2 and Cat3 knockout mutants under salt stress

2016 ◽  
Vol 14 (1) ◽  
pp. 3-7 ◽  
Author(s):  
N. O. Didenko ◽  
I. M. Buzduga ◽  
R. A. Volkov ◽  
I. I. Panchuk
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Sodium Chloride ◽  
Cellular Response ◽  
Resistance Mechanisms ◽  
Stress Conditions ◽  
Free Proline ◽  
Knockout Mutants ◽  
Knockout Line

Aim. In plants, the enzymatic and non-enzymatic environmental stress resistance mechanisms function in a concerted manner, but the role of specific isoforms of antioxidant enzymes and their relationship to low molecular weight protective compounds is poorly understood. To investigate this question free proline levels were compared under salt stress conditions in wild-type Arabidopsis thaliana and knockout mutants for the catalase genes Cat2 and Cat3. Methods. Free proline content was measured under various treatments of plants with sodium chloride. Results. It was shown that under salt stress conditions free proline increase is impaired in leaves of mutants lacking CAT2 and CAT3 activity. In addition, cat3 knockout line shows a reduced basal level of proline under non-stress conditions. Conclusions. Suppression of response to salt stress points to a possible link between the rearrangement of the antioxidant system in the Cat genes knockouts and the regulation of protective cellular response to salt stress.Key words: Arabidopsis thaliana, knockout mutants, catalase, proline, sodium chloride.

scholarly journals Alterations of Lysine Modifications on the Histone H3 N-Tail under Drought Stress Conditions in Arabidopsis thaliana

2009 ◽  
Vol 50 (10) ◽  
pp. 1856-1856 ◽  
Author(s):  
J.-M. Kim ◽  
T. K. To ◽  
J. Ishida ◽  
T. Morosawa ◽  
M. Kawashima ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Histone H3 ◽  
Stress Conditions

scholarly journals F-box protein At1g08710 negatively regulates root length and imparts drought stress tolerance in Arabidopsis thaliana

2020 ◽  
Author(s):  
Venkateswara Rao ◽  
Virupapuram Vijaybhaskar
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Stress Conditions ◽  
Stress Adaptation ◽  
List Type ◽  
E3 Ligases ◽  
Drought Stress Tolerance ◽  
Activator Protein ◽  
F Box Protein

AbstractPlants experience abiotic stresses throughout their life cycle and accordingly respond to tide over the unfavorable conditions. Drought or water deficit is one such condition to which plants respond in various ways including the ubiquitin proteasome system (UPS). Ubiquitin E3 ligases are a diverse family of protein complexes of which Skp1□Cullin□F□box (SCF) class mediate the ubiquitination and subsequent proteolytic turnover of proteins. F□box protein subunit plays crucial role in imparting specificity for selective degradation of target proteins. Here we report the function of Arabidopsis F-box protein At1g08710 in drought stress adaptation. As F-box protein is a constituent of SCF complex, here it is shown interacting with ASK1 and Cullin1. F-box protein localizes to both nucleus and membrane. F-box gene transcript accumulates highly in root and is altered in response to drought stress conditions. F-box protein interacts with a transcriptional co-activator protein ADA2b. F-box mutant plants displayed better growth under drought stress conditions compared to the wild type with a reduced accumulation of H2O2 and malondialdehyde (MDA). Drought responsive genes RD29A, RD22, ABI3 expression is also induced in F-box mutant plants. These results indicate F-box protein At1g08710 plays a role in drought stress adaptation in Arabidopsis thaliana.HighlightsF-box gene At1g08710 encodes a nuclear, membrane localized protein.F-box protein At1g08710 interacts with transcriptional co-activator protein ADA2b.F-box protein imparts drought stress tolerance by modulating H2O2 and MDA content.Drought response genes RD29A, RD22, ABI3 expression is induced in F-box mutant plants.

Tissue specificity of expression of heat shock gene ATHSP70-10 in Arabidopsis thaliana seedlings under normal and stress conditions

2020 ◽  
Vol 2020 (3) ◽  
pp. 37-47
Author(s):  
L.Ye. Kozeko ◽  
◽  
E.L. Kordyum ◽  
Keyword(s):  
Arabidopsis Thaliana ◽  
Heat Shock ◽  
Water Deficit ◽  
Tissue Specificity ◽  
Abiotic Factors ◽  
Root Apex ◽  
Stress Conditions ◽  
Heat Shock Gene ◽  
Organ Specificity ◽  
Pcr Analysis

Mitochondrial heat shock proteins of HSP70 family support protein homeostasis in mitochondria under normal and stress conditions. They provide folding and complex assembly of proteins encoded by mitochondrial genome, as well as import of cytosolic proteins to mitochondria, their folding and protection against aggregation. There are reports about organ-specificity of mitochondrial HSP70 synthesis in plants. However, tissue specificity of their functioning remains incompletely characterized. This problem was studied for mitochondrial AtHSP70-10 in Arabidopsis thaliana seedlings using a transgenic line with uidA signal gene under normal conditions, as well as high temperature and water deficit. Under normal conditions, histochemical GUS-staining revealed the expression of AtHSP70-10 in cotyledon and leaf hydathodes, stipules, central cylinder in root differentiation and mature zones, as well as weak staining in root apex and root-shoot junction zone. RT-PCR analysis of wild-type seedlings exposed to 37°C showed rapid upregulation of AtHSP70-10, which reached the highest level within 2 h. In addition, the gradual development of water deficit for 5 days caused an increase in transcription of this gene, which became more pronounced after 3 days and reached a maximum after 5 days of dehydration. Histochemical analysis showed complete preservation of tissue localization of AtHSP70-10 expression under both abiotic factors. The data obtained indicate the specific functioning of mitochondrial chaperone AtHSP70-10 in certain plant cellular structures.

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