Background:
The occurrence of Cancer results in cellular changes that causes the uncontrolled growth and
division of cells. Apoptosis removes cells during development and eliminates the potentially cancerous cells. The bioactive
compounds present in the herbal plant shows cytotoxic activity that result in apoptosis. The traditional herbal plants are
used world-wide both in allopathy and other traditional ways.
Objective:
The main objective of this study is to extract the bioactive compound Quercetin from the medicinally significant
plant Ocimum sanctum and also to develop nanomedicine as Qu-PEG-NiGs.
Materials and Methods:
Leaf extract of the medicinally significant plant Ocimum sanctum (O. sanctum) has been used for
the synthesis of nickel nanoparticles (NiGs) and extraction of quercetin (Qu). The ethanolic extract of Ocimum sanctum is
added to 1 mM Nickel Nitrate (Ni(NO3)2) and stirred for 3 hrs at RT and dried at 60°C for 3hrs and calcinated at 400°C for
2hrs and characterized using Uv-Vis Spectrophotometer, FT-IR, SEM, DLS and Zeta potential. The Quercetin is isolated
from Ocimum sanctum leaf extract using the reflux condenser method. The bio-polymer is being PEG-coated over NiGs
and Quercetin is loaded into it. The apoptosis activity using MCF-7 cells is performed with Qu-PEG-NiGs. The purity of
Quercetin is characterized using HPLC. In order to analyse apoptosis efficiency, MTT assay, Reactive Oxygen Species
(ROS), Cell cycle analysis has been performed.
Results:
The NiGs absorption spectrum gives a peak at 408nm. The FT-IR confirms the presence of particular functional
groups shifting from the compound NiGs and then coated with PEG-Qu-NiGs. The SEM images show the size of NiGs
ranging from 27.3 nm to 40.4 nm with varied morphology such as hexagonal and other irregular shapes. The presence of
Quercetin extracted from the leaf powder is approximately 1.5 mg/g. The ROS results show the Qu-PEG-NiGs induced
efficiency of the apoptosis, while the increased concentrations promote ROS and lead to activation of the apoptosis. The
cell cycle analysis has shown the cytotoxic effect.
Conclusion:
PEG-coated nickel nanoparticles can be used as a promising chemotherapeutic agent against MCF7 breast
cancer cells. It is the evidence to further studies for evaluating Qu-PEG-NiGs anticancer activity on different types of cancer
cells.
Simultaneous determination of catechol and hydroquinone using nickel nanoparticles-4-nitroaniline nanocomposite modified glassy carbon electrode
