scholarly journals Characterization of Novel Cement-Based Carboxymethyl Chitosan/Amorphous Calcium Phosphate

2022 ◽  
Author(s):  
Aditya Wisnu Putranto ◽  
Endang Suprastiwi ◽  
Ratna Meidyawati ◽  
Harry Agusnar
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Setting Time ◽  
Milled Powder ◽  
Analysis Data ◽  
Carboxymethyl Chitosan ◽  
Sem Images ◽  
Setting Times ◽  
Parametric Data ◽  
Post Hoc

Abstract Objective This study aimed to analyze, evaluate, and characterize novel cement-based carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP). Materials and Methods The three cement groups studied were gypsum (Gyp), and CMC/ACP—gypsum cement-based 5% (5% CAG) and 10% (10% CAG). The groups were characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), setting time, and scanning electron microscopy (SEM) data. The characterization results were analyzed qualitatively, but the data for setting time were analyzed using SPSS (p < 0.05). Statistical Analysis Data were statistically analyzed. One-way analysis of variance was used to compare numerical (parametric) data between more than two separate groups followed by post hoc Tukey. Results FTIR showed phosphate groups indicate the presence of calcium phosphate in the form of amorphous (ACP) in the CMC/ACP, CMC/ACP post-milled powder, and CMC/ACP cement-based (5% CAG and 10% CAG). XRD showed no difference in the diffraction spectra among the Gyp, 5% CAG, and 10% CAG groups. SEM images revealed that the CMC/ACP cement-based groups (5% CAG and 10% CAG) showed CMC/ACP cluster filled with hollow spaces between the gypsum crystals and aggregations surrounding the gypsum crystals. The CMC/ACP showed envelopes and attached to the crystalline structures of the gypsum. Setting times of 5% CAG and 10% CAG showed significant differences compared with Gyp (p < 0.05). Conclusion The result of our study showed that CMC/ACP cement-based (5% CAG and 10% CAG) demonstrated amorphous characteristic, which can stabilize calcium ions and phosphate group (ACP). In addition, the modification of gypsum using CMC/ACP as cement-based extended the time of setting.

Preparation of Calcium Phosphate Cement Utilizing Dicalcium Phosphate Dihydrate and Calcium Carbonate

2014 ◽  
Vol 608 ◽  
pp. 280-286
Author(s):  
Nudthakarn Kosachan ◽  
Angkhana Jaroenworaluck ◽  
Sirithan Jiemsirilers ◽  
Supatra Jinawath ◽  
Ron Stevens
Keyword(s):  
Calcium Carbonate ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Raw Materials ◽  
Setting Time ◽  
Dicalcium Phosphate ◽  
Dicalcium Phosphate Dihydrate ◽  
Cement Pastes ◽  
Setting Times ◽  
Phosphate Dihydrate

Calcium phosphate cement has been widely used as a bone substitute because of its chemical similarity to natural bone. In this study, calcium phosphate cement was prepared using dicalcium phosphate dihydrate (CaHPO4.2H2O) and calcium carbonate (CaCO3) as starting raw materials. The cement pastes were mixed and the chemistry adjusted with two different aqueous solutions of sodium hydroxide (NaOH) and disodium hydrogen phosphate (Na2HPO4). Concentrations of the solution were varied in the range 0.5 to 5.0 mol/L with the ratio of solid/liquid = 2 g/ml. The cement paste was then poured into a silicone mold having a diameter of 10 mm and a height 15 mm. Setting times for the cement were measured using a Vicat apparatus. XRD, FT-IR, and SEM techniques were used to characterize properties and microstructure of the cement. From the experimental results, it is clear that different concentrations of Na2HPO4 and NaOH have affected the setting times of the cement. The relationship between concentration of NaOH and Na2HPO4 and setting time, including final properties of the cement, is discussed.

scholarly journals Effect of Galla Chinensis on Remineralization of Early Dentin Lesion

2020 ◽  
Vol 14 (04) ◽  
pp. 651-656
Author(s):  
Hend Mahmoud Abdel-Azem ◽  
Ahmed Fawzy Abo Elezz ◽  
Rehab Khalil Safy
Keyword(s):  
Treatment Time ◽  
Analysis Data ◽  
Environmental Scanning ◽  
X Ray ◽  
Parametric Data ◽  
Significant Difference ◽  
Ph Cycling ◽  
The Mean ◽  
Post Hoc

Abstract Objective This study aimed to investigate the efficacy of the Galla chinensis extract (GCE) on early dentin lesion remineralization. Materials and Methods Seventy-two dentin specimens were divided into three groups according to the treatment solution. In group S1, specimens were treated with GCE; in group S2, the specimens were treated with sodium fluoride (NaF); meanwhile, specimens of group S3 were treated with distilled water (DW). Each group was further subdivided into two subgroups according to the treatment time (1 minute and 5 minutes). An in vitro pH-cycling model for 12 days was done. Subsequently, surface microhardness (SMH) of the specimens, elemental analysis, and their micromorphological appearance were evaluated. Statistical Analysis Data were statistically analyzed. One-way analysis of variance was used to compare numerical (parametric) data between more than two separate groups followed by post hoc Tukey. Results There was no significant difference between the mean SMH of dentin between NaF and GCE groups. Regarding the time, the 5-minute treatment with NaF and DW groups recorded higher mean SMH value of dentin than the 1-minute treatment group. Meanwhile, for GCE groups, the 1-minute immersion recorded higher mean SMH value than the 5-minute immersion without any significant difference between them. The microhardness results were confirmed by environmental scanning electron microscope and energy dispersive X-ray analysis results. Conclusion GCE could be used as an effective alternative for dentin remineralization.

Preparation and Characterization of Silk Fibroin/Calcium Phosphate Composite

2011 ◽  
Vol 332-334 ◽  
pp. 1655-1658
Author(s):  
Biao Wang ◽  
Rui Juan Xie ◽  
Yang Yang Huang
Keyword(s):  
Compressive Strength ◽  
Calcium Phosphate ◽  
Silk Fibroin ◽  
Solid Phase ◽  
Setting Time ◽  
Testing Machine ◽  
X Ray Diffraction ◽  
Acicular Crystal ◽  
Setting Times

In this paper, calcium phosphate cement (CPC) was prepared with tetracalcium phosphate (TTCP)/dicalcium phosphate anhydrous (DCPA) system as solid phase and phosphate buffered solution (PBS) as liquid phase, then silk fibroin (SF) was added into CPC to form silk fibroin/calcium phosphate composite. To study the effect of SF on the properties of composite, different mass fraction of SF was added into the composite. The surface morphology was observed by Scanning Electron Microscope. The setting time was investigated by ISO Cement Standard Consistency Instrument. The structure of the composite was studied by X-ray diffraction and infrared spectroscopy. Mechanical properties of samples were tested by Instron Universal Testing Machine. The results showed that the particles of SF could be seen obviously in the surface of all composite, and acicular crystal of hydroxyapatite (HA) was formed in the hardening body of both the composite and the pure CPC. The acicular crystal of HA derived from composite with SF appeared to be thinner. The setting times of the composites were all between 9 to 15min. Compared to pure CPC, the compressive strength and work-of-compressive of composites were all improved. The compressive strength of the composite with 1% SF increased obviously.

scholarly journals Incorporation of Poly(Vinyl Alcohol) for The Improved Properties of Hydrothermal Derived Calcium Phosphate Cements

2018 ◽  
Vol 18 (2) ◽  
pp. 354
Author(s):  
Nurul Nabilah Razali ◽  
Iis Sopyan
Keyword(s):  
Calcium Phosphate ◽  
Vinyl Alcohol ◽  
Setting Time ◽  
Poly Vinyl Alcohol ◽  
Dihydrogen Phosphate ◽  
Distilled Water ◽  
Ammonium Dihydrogen Phosphate ◽  
Hydrothermal Route ◽  
Ringer’S Solution ◽  
Setting Times

Calcium phosphate cement (CPC) has been synthesized via a straightforward hydrothermal route. Calcium oxide and ammonium dihydrogen phosphate were used as calcium and phosphate precursors. The precursors were refluxed in distilled water at 90–100 °C and dried overnight until the calcium phosphate powder was formed. CPC was then produced by mixing the powder and distilled water at the powder-to-liquid (P/L) ratio of 1.5. Poly(vinyl alcohol) (PVA) of 1 to 7% (w/w) was added and its effect on physical properties was investigated. It was proved that PVA addition up to 7% (w/w) has shortened the setting time but decreased the injectability. The PVA free CPC has the initial and final setting times of 71 and 187 min, respectively and the injectability of 99.92%. The compressive strength also increased with the amount of PVA added in CPC. In addition, soaking CPC in Ringer's solution for 7, 14 and 21 days also gave remarkable effects on cohesion, microstructure and mechanical properties of the cement.

Physicochemical and Mechanical Properties of Composite of Chitosan Microspheres/Calcium Phosphate Cement

2007 ◽  
Vol 336-338 ◽  
pp. 1654-1657
Author(s):  
Rui Liu ◽  
Li Min Dong ◽  
Qing Feng Zan ◽  
Chen Wang ◽  
Jie Mo Tian
Keyword(s):  
Mechanical Properties ◽  
Compressive Strength ◽  
Calcium Phosphate ◽  
Bone Cement ◽  
Setting Time ◽  
Chemical Properties ◽  
Phase Evolution ◽  
Chitosan Microspheres ◽  
Setting Times ◽  
Calcium Phosphate Bone Cement

The aim of this work is to improve the mechanical properties of calcium phosphate bone cement (CPC) by appending chitosan microspheres to CPC base. That chitosan degrades rapidly than bone cement has been proved by previous investigations. Porous CPC has low compressive strength because of the pores in it weakening the structure. Additive chitosan microspheres can improve the mechanical properties by bearing the compress with the CPC base and produce pores after degradation. This study investigates the effect of chitosan microspheres on the setting time, mechanical properties, phase evolution and morphology of CPC. The additive proportion of chitosan microspheres ranges from 0 wt% to 30 wt%. Compared with original CPC, the modified CPC has higher compressive strength, without significantly affecting the chemical properties. The phase composition of the CPC is tested by XRD. The microstructures of CPC are observed using SEM. The final setting times range from 5~15 minutes and can be modulated by using different liquid and powder (L/P) ratio.

scholarly journals THE EFFECT OF CARBOXYMETHYL CHITOSAN/AMORPHOUS CALCIUM PHOSPHATE TO GUIDE TISSUE REMINERALIZATION OF DENTIN COLLAGEN

2019 ◽  
pp. 181-183
Author(s):  
ROSDIANA NURUL ANNISA ◽  
NILAKESUMA DJAUHARIE ◽  
ENDANG SUPRASTIWI ◽  
NORMA AVANTI
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Matrix Protein ◽  
Carboxymethyl Chitosan ◽  
Control Group ◽  
Group 14 ◽  
Tem Analysis ◽  
Transmission Electron ◽  
Dentin Collagen

Objective: Carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP) can replace the role of dentine matrix protein 1. Guided tissueremineralization (GTR) is a method of extrafibrillar and intrafibrillar collagen remineralization. This study analyzed the ability of CMC/ACP to achieveintrafibrillar and extrafibrillar remineralization on demineralized dentin.Methods: We divided 12 demineralized occlusal cavities into four groups: 7 days control group, 14 days control group, 7 days CMC/ACP group, and14 days CMC/ACP group. In the control group, the cavities were directly filled with temporary restoration material, while the cavities of the CMC/ACPgroups first underwent application of CMC/ACP and were then filled with temporary restoration material. All samples were stored in a 37°C shakingincubator. 12 samples were analyzed by energy-dispersive X-ray (EDX) spectroscopy and four were analyzed by transmission electron microscope(TEM).Results: From day 7 to day 14, there was a significant increase in calcium and phosphate levels within the CMC/APC groups. The phosphate levelswere much lower than the calcium or minerals formed on the 7th day, in the form of hydroxyapatite. TEM analysis shows that the CMC/ACP groupexhibited more intrafibrillar and extrafibrillar remineralization.Conclusion: CMC/ACP can improve the GTR process.

scholarly journals CONCENTRATION DEPENDENT EFFECTS OF CARBOXYMETHYL CHITOSAN ON DENTIN REMINERALIZATION WITH AMORPHOUS CALCIUM PHOSPHATE

2020 ◽  
pp. 31-33
Author(s):  
HASTI DWI SETIATI ◽  
ENDANG SUPRASTIWI ◽  
DEWA AYU NYOMAN PUTRI ARTININGSIH ◽  
LUH PUTU TRISNA BUDI UTAMI
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Matrix Protein ◽  
Carboxymethyl Chitosan ◽  
Control Group ◽  
Dentin Matrix Protein ◽  
Group 4 ◽  
Occlusal Surfaces ◽  
Group 5 ◽  
Group 2

Objective: Carboxymethyl chitosan (CMC) is a non-collagenous protein analog which has a similar role as dentin matrix protein 1. CMC stabilizes amorphous calcium phosphate (ACP); hence forming nanocomplexes of CMC-ACP. The purpose of this study was to evaluate the effect of CMC concentration in CMC-ACP on dentin remineralization.Methods: Cavities were formed on the occlusal surfaces of freshly extracted premolar teeth. All samples were demineralized and immersed in phosphate-buffered saline and stored in a shaking incubator at 37°C. The teeth were randomly divided into five groups. Group 1 was control group (no treatment), whereas Groups 2, 3, 4, and 5 were treated with CMC-ACP containing 1%, 2.5%, 5%, and 10% CMC. The remineralized layer on the dentin surface was evaluated using scanning electron microscopy and energy-dispersive X-ray analysis.Results: The highest dentin remineralization capacity was achieved in Group 5 (10% CMC), whereas diminishing effects were observed in Group 4 (5% CMC), Group 3 (2.5% CMC), and Group 2 (1% CMC). Although no significant differences in calcium levels were observed between 2.5%, 5%, and 10% CMC groups, phosphate levels differed significantly in all treatment groups.Conclusion: Optimal dentin remineralization was achieved by the application of CMC–ACP containing 2.5% CMC.

scholarly journals THE EFFECTS OF MIXTURES OF VARIOUS CONCENTRATIONS OF CARBOXYMETHYL CHITOSAN/AMORPHOUS CALCIUM PHOSPHATE WITH GYPSUM ON DENTIN REMINERALIZATION

2020 ◽  
pp. 13-15
Author(s):  
IKE DWI MAHARTI ◽  
ENDANG SUPRASTIWI ◽  
HASTI DWI SETIATI ◽  
ERLIYANA YAMIN ◽  
ANDI NURWALINI CAHYANI
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Carboxymethyl Chitosan ◽  
Phosphate Concentration ◽  
X Ray ◽  
Compound Material ◽  
Collagen Protein ◽  
One Way Anova ◽  
Demineralized Dentin ◽  
Scanning Electron

Objective: Carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP) is a non-collagen protein analog with the ability of dentinremineralization, and gypsum is a compound material than can facilitate application of CMC/ACP. CMC/ACP concentration is claimed to increasecalcium and phosphate concentration, which indicates dentin remineralization. The aim of this study is to observe the effects of different concentrationsof CMC/ACP mixed with gypsum on dentin remineralization.Methods: Occlusal cavities were created in 18 freshly extracted teeth, and the teeth were divided into three groups. The first group consisted ofuntreated demineralized dentin, and the second and third groups were treated with mixtures of 2.5% and 5% CMC/ACP and gypsum. All groupswere checked and analyzed on the 14th day using scanning electron microscopy/energy-dispersive X-ray spectroscopy and one-way ANOVA (p<0.05).Results: Significant differences (p=0.001) of calcium and phosphate concentrations were found between Groups I, II, and III (p=0.001), whereas nodifferences were found between Groups II and III.Conclusion: Gypsum and CMC/ACP work synergistically to induce dentin remineralization. The highest dentin remineralization was shown by 5%CMC/ACP-gypsum mixture, but the level was not statistically different from that of 2.5% CMC/ACP-gypsum mixture.

scholarly journals Setting time of construction gypsum, dental plaster, and white orthodontic gypsum

2020 ◽  
Vol 14 (3) ◽  
pp. 167-170
Author(s):  
Imelda Darmawan ◽  
Octarina Willy ◽  
Johan Arief Budiman
Keyword(s):  
Setting Time ◽  
Essential Property ◽  
Initial Setting Time ◽  
Final Setting Time ◽  
Setting Times ◽  
Initial Setting ◽  
Experimental Laboratory ◽  
Dental Plaster ◽  
Post Hoc ◽  
One Way Anova

Background. Dental plaster, white orthodontic gypsum, and construction gypsum have β-hemihydrate particles. Setting time is an essential property of dental gypsum, which can affect the strength of the material. This research aimed to compare construction gypsum, dental plaster, and white orthodontic gypsum’s initial and final setting times. Methods. Three groups were included in this experimental laboratory study: construction gypsum (A), dental plaster (B), and white orthodontic gypsum (C). Each group consisted of 10 samples. Gypsum manipulation consisted of using 120 gr of powder and 60 mL of water. Gypsum powder and water were mixed using a gypsum mixer at 120 rpm. A homogeneous mixture was poured into a mold, and the setting time was measured using a Gillmore needle, according to ASTM C266-03. The initial setting time test was measured using 113.4 grams and a 2.12-mm needle. The final setting time was measured using 453.6 grams and a 1.06-mm needle. This test was repeated until the needle failed to penetrate the gypsum’s surface. All the data were analyzed with one-way ANOVA and post hoc Tukey tests using SPSS 23. Results. The average initial setting time for groups A, B, and C were 10.39±1.19, 16.17±1.40, and 24.46±1.51, respectively. The average final setting time for groups A, B, and C were 15.97±0.79, 24.31±0.88) and 33.37±0.66, respectively. One-way ANOVA and post hoc Tukey tests showed significant differences in the initial and final setting times between the three groups (P<0.05). Conclusion. There were differences in setting time between dental plaster, white orthodontic gypsum, and construction gypsum. The construction gypsum’s setting time is suitable as a type II dental gypsum, according to ADA No.25.

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