Influence of the measurement configuration on the results of Raman microspectroscopy of human hair

2022 ◽  
Vol 52 (1) ◽  
pp. 36-41
Author(s):  
N N Brandt ◽  
E I Travkina ◽  
E V Mikhal'chik ◽  
A Yu Chikishev
Keyword(s):  
Human Hair ◽  
Optical Axis ◽  
Spectroscopic Studies ◽  
Original Method ◽  
Raman Microspectroscopy ◽  
Measuring Setup ◽  
Experimental Facilities ◽  
Accuracy Of Measurement ◽  
Confocal Raman Microspectroscopy ◽  
Chemical Influence

Abstract Increasing interest in spectroscopic studies of human hair raises the question about the accuracy of measurement of their spectra and requires optimisation of experimental facilities. An original method of obtaining transverse hair sections without using a microtome and chemical influence is proposed. The results obtained by confocal Raman microspectroscopy of human hair differently oriented with respect to the optical axis of the measuring setup are compared. It is shown that, in addition to expected changes in the spectra measured at different distances from the hair periphery in the direction to its centre, the spectra measured in the case of hair excitation perpendicular and parallel to its axis are also considerably different.

Raman spectroscopic studies of vitamin A content in the liver: a biomarker of healthy liver

The Analyst ◽  
2015 ◽  
Vol 140 (7) ◽  
pp. 2074-2079 ◽  
Author(s):  
K. Kochan ◽  
K. M. Marzec ◽  
E. Maslak ◽  
S. Chlopicki ◽  
M. Baranska
Keyword(s):  
Vitamin A ◽  
Hepatic Stellate Cells ◽  
Liver Steatosis ◽  
Stellate Cells ◽  
Spectroscopic Studies ◽  
Raman Microspectroscopy ◽  
Raman Spectroscopic ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman ◽  
Healthy Liver

Confocal Raman microspectroscopy was used in this study to identify hepatic stellate cells (HSCs) from healthy mice and mice with untreated and treated liver steatosis.

Confocal Raman Microspectroscopy Using a Stigmatic Spectrograph and CCD Detector

1994 ◽  
Vol 48 (2) ◽  
pp. 232-235 ◽  
Author(s):  
K. P. J. Williams ◽  
G. D. Pitt ◽  
D. N. Batchelder ◽  
B. J. Kip
Keyword(s):  
Focal Plane ◽  
Optical Axis ◽  
Depth Resolution ◽  
Raman Microspectroscopy ◽  
Ccd Detector ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

Confocal Raman microspectroscopy has previously used pinholes placed at the back focal plane of the microscope to provide depth resolution along the optical axis. The process of optimizing the pinhole alignment can often be difficult and time-consuming. We demonstrate a different approach to setting up a confocal Raman microscope using a stigmatic spectrograph and a CCD detector. This arrangement is easy to use and provides a depth resolution of ∼2 μm.

Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa

The Analyst ◽  
2010 ◽  
Vol 135 (6) ◽  
pp. 1370 ◽  
Author(s):  
Konrad Meister ◽  
Diedrich A. Schmidt ◽  
Erik Bründermann ◽  
Martina Havenith
Keyword(s):  
Raman Microspectroscopy ◽  
Analytical Tool ◽  
Human Spermatozoa ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

scholarly journals Molecular Composition Of Organelles In A Living Yeast Cell Studied By Confocal Raman Microspectroscopy

2003 ◽  
Vol 9 (S02) ◽  
pp. 1082-1083
Author(s):  
Yu-San Huang ◽  
Takeshi Karashima ◽  
Masayuki Yamamoto ◽  
Hiro-o Hamaguchi
Keyword(s):  
Yeast Cell ◽  
Raman Microspectroscopy ◽  
Molecular Composition ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

Assessment of the “Skin Reservoir” of Urea by Confocal Raman Microspectroscopy and Reverse Iontophoresis in vivo

2007 ◽  
Vol 24 (10) ◽  
pp. 1897-1901 ◽  
Author(s):  
Valentine Wascotte ◽  
Peter Caspers ◽  
Johanna de Sterke ◽  
Michel Jadoul ◽  
Richard H. Guy ◽  
...  
Keyword(s):  
Raman Microspectroscopy ◽  
Reverse Iontophoresis ◽  
Skin Reservoir ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

Correction of optical distortions in dry depth profiling with confocal Raman microspectroscopy

2011 ◽  
Vol 42 (6) ◽  
pp. 1330-1334 ◽  
Author(s):  
J. Pablo Tomba ◽  
María de la Paz Miguel ◽  
Claudio J. Perez
Keyword(s):  
Depth Profiling ◽  
Raman Microspectroscopy ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

Confocal Raman microspectroscopy of the skin

2011 ◽  
Vol 21 (6) ◽  
pp. 851-863 ◽  
Author(s):  
Matthias Förster ◽  
Marie-Alexandrine Bolzinger ◽  
Gilles Montagnac ◽  
Stéphanie Briançon
Keyword(s):  
Raman Microspectroscopy ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman
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