scholarly journals Phase separation in solutions of monoclonal antibodies and the effect of human serum albumin

2011 ◽  
Vol 108 (40) ◽  
pp. 16606-16611 ◽  
Author(s):  
Y. Wang ◽  
A. Lomakin ◽  
R. F. Latypov ◽  
G. B. Benedek
Keyword(s):  
Monoclonal Antibodies ◽  
Phase Separation ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum

Study of the antigenic structure of human serum albumin with monoclonal antibodies

1985 ◽  
Vol 22 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Noëlle Doyen ◽  
Claude Lapresle ◽  
Pierre Lafaye ◽  
Jean-Claude Mazie
Keyword(s):  
Monoclonal Antibodies ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Antigenic Structure

scholarly journals One-Step Preparation of Antifouling Polysulfone Ultrafiltration Membranes via Modification by a Cationic Polyelectrolyte Based on Polyacrylamide

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1017 ◽  
Author(s):  
Tatiana V. Plisko ◽  
Alexandr V. Bildyukevich ◽  
Katsiaryna S. Burts ◽  
Sergey S. Ermakov ◽  
Anastasia V. Penkova ◽  
...  
Keyword(s):  
Phase Separation ◽  
Human Serum Albumin ◽  
Zeta Potential ◽  
Serum Albumin ◽  
Human Serum ◽  
Cationic Polyelectrolyte ◽  
Coagulation Bath ◽  
Ultrafiltration Membranes ◽  
Selective Layer ◽  
One Step

A novel method for one-step preparation of antifouling ultrafiltration membranes via a non-solvent induced phase separation (NIPS) technique is proposed. It involves using aqueous 0.05–0.3 wt.% solutions of cationic polyelectrolyte based on a copolymer of acrylamide and 2-acryloxyethyltrimethylammonium chloride (Praestol 859) as a coagulant in NIPS. A systematic study of the effect of the cationic polyelectrolyte addition to the coagulant on the structure, performance and antifouling stability of polysulfone membranes was carried out. The methods for membrane characterization involved scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), contact angle and zeta-potential measurements and evaluation of the permeability, rejection and antifouling performance in human serum albumin solution and surface water ultrafiltration. It was revealed that in the presence of cationic polyelectrolyte in the coagulation bath, its concentration has a major influence on the rate of “solvent–non-solvent” exchange and thus also on the rate of phase separation which significantly affects membrane structure. The immobilization of cationic polyelectrolyte macromolecules into the selective layer was confirmed by FTIR spectroscopy. It was revealed that polyelectrolyte macromolecules predominately immobilize on the surface of the selective layer and not on the bottom layer. Membrane modification was found to improve the hydrophilicity of the selective layer, to increase surface roughness and to change zeta-potential which yields the substantial improvement of membrane antifouling stability toward natural organic matter and human serum albumin.

Conformational analysis of human serum albumin and its non-enzymatic glycation products using monoclonal antibodies

2011 ◽  
Vol 149 (5) ◽  
pp. 569-580 ◽  
Author(s):  
Keigo Saito ◽  
Kuniko Hamano ◽  
Masatoshi Nakagawa ◽  
Keiko Yugawa ◽  
Jin Muraoka ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Conformational Analysis ◽  
Human Serum

Chromatographic kinetic measurements of human serum albumin adsorption on monoclonal antibodies

1995 ◽  
Vol 8 (1-2) ◽  
pp. 85-89 ◽  
Author(s):  
James Renard ◽  
Claire Vidal-Madjar ◽  
Bernard Sebille ◽  
Claude Lapresle
Keyword(s):  
Monoclonal Antibodies ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Kinetic Measurements ◽  
Albumin Adsorption

scholarly journals Effect of Polyphenylsulfone and Polysulfone Incompatibility on the Structure and Performance of Blend Membranes for Ultrafiltration

Materials ◽  
2021 ◽  
Vol 14 (19) ◽  
pp. 5740
Author(s):  
Tatiana Plisko ◽  
Yana Karslyan ◽  
Alexandr Bildyukevich
Keyword(s):  
Phase Separation ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Pure Water ◽  
Water Flux ◽  
Ultrafiltration Membranes ◽  
Casting Solution ◽  
Blend Membranes ◽  
Pure Water Flux

This study deals with the modification of polyphenylsulfone ultrafiltration membranes by introduction of an incompatible polymer polysulfone to the polyphenylsulfone casting solution to improve the permeability. The correlation between properties of the blend polyphenylsulfone/polysulfone solutions and porous anisotropic membranes for ultrafiltration prepared from these solutions was revealed. The blend polyphenylsulfone/polysulfone solutions were investigated using a turbidity spectrum method, optical microscopy and measurements of dynamic viscosity and turbidity. The structure of the prepared blend flat sheet membranes was studied using scanning electron microscopy. Membrane separation performance was investigated in the process of ultrafiltration of human serum albumin buffered solutions. It was found that with the introduction of polysulfone to the polyphenylsulfone casting solution in N-methyl-2-pyrrolidone the size of supramolecular particles significantly increases with the maximum at (40–60):(60:40) polyphenylsulfone:polysulfone blend ratio from 76 nm to 196–354 nm. It was shown that polyphenylsulfone/polysulfone blend solutions, unlike the solutions of pristine polymers, are two-phase systems (emulsions) with the maximum droplet size and highest degree of polydispersity at polyphenylsulfone/polysulfone blend ratios (30–60):(70–40). Pure water flux of the blend membranes passes through a maximum in the region of the most heterogeneous structure of the casting solution, which is associated with the imposition of a polymer-polymer phase separation on the non-solvent induced phase separation upon membrane preparation. The application of polyphenylsulfone/polysulfone blends as membrane-forming polymers and polyethylene glycol (Mn = 400 g·mol−1) as a pore-forming agent to the casting solutions yields the formation of ultrafiltration membranes with high membrane pure water flux (270 L·m−2·h−1 at 0.1MPa) and human serum albumin rejection of 85%.

II. Lokalisation der Placenta mit 113Inm- Human-Serum-Albumin

1969 ◽  
Vol 08 (01) ◽  
pp. 15-21 ◽  
Author(s):  
K. E. Scheer ◽  
J. Heep ◽  
W. Maier-Borst ◽  
W. J. Lorenz ◽  
H. Sinn ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Vena Cava ◽  
Placenta Praevia ◽  
Wird Eine

ZusammenfassungNach tierexperimentellen Voruntersuchungen wurde die Placentographie mit trägerfreiem 113Inm -HSA als klinische Methode eingeführt. Vor Amniocentesen und bei Verdacht auf Placenta praevia werden Placentographien geschrieben. Den Schwangeren wird eine Aktivität von 500 μCi in die Cubitalvene injiziert. Die der Aktivität entsprechende Indiummenge ist kleiner als 0,1 ng. Die fetale Strahlenbelastung liegt unter lOmrad. Bei Anwendung von 113Inm-HSA entfällt eine Blockade der mütterlichen und fetalen Schilddrüsen. Die genaue Abgrenzung einer Placenta praevia wird nicht durch eine Blasenaktivität beeinträchtigt.Es wurden bisher 19 Placentalokalisationen durchgeführt. In allen Fällen konnte der Placentasitz eindeutig festgestellt werden. Bedingt durch die lange Liegezeit beim Aufnehmen eines Szintigramms kam es in zwei Fällen zu einem Vena-Cava-Kompressions-Syndrom. Zur Verhinderung dieser klinischen Zwischenfälle werden inzwischen Placentographien mit der Anger-Kamera aufgenommen. Mit Hilfe des divergierenden Kollimators konnte der gesamte Abdominalbereich erfaßt werden. Die Aufnahmezeit konnte auf 7 — 10 Minuten verkürzt werden. Die intravenöse injizierte Aktivität betrug bei dieser Methode ebenfalls 500 μCi. Der diagnostische Aussagewert der Kamerabilder ist szintigraphischen Aufnahmen gleichwertig.

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