scholarly journals Morphologic and antigenic characterization of interferon gamma-mediated persistent Chlamydia trachomatis infection in vitro.

1993 ◽  
Vol 90 (9) ◽  
pp. 3998-4002 ◽  
Author(s):  
W. L. Beatty ◽  
G. I. Byrne ◽  
R. P. Morrison
Keyword(s):  
Chlamydia Trachomatis ◽  
Interferon Gamma ◽  
Chlamydia Trachomatis Infection ◽  
Antigenic Characterization

scholarly journals Identification and Characterization of P0 Protein as a Vaccine Candidate Against Babesia divergens, Blood Parasite of Veterinary and Zoonotic Importance

2022 ◽  
Vol 8 ◽  
Author(s):  
Shimaa Abd El-Salam El-Sayed ◽  
Mohamed Abdo Rizk ◽  
Haitham Eldoumani ◽  
Shimaa Sobhy Sorour ◽  
Mohamad Alaa Terkawi ◽  
...  
Keyword(s):  
Vaccine Candidate ◽  
Fluorescent Antibody ◽  
Specific Interaction ◽  
Blood Parasite ◽  
Antigenic Characterization ◽  
Babesia Divergens ◽  
Field Samples ◽  
P0 Protein

The molecular identification and antigenic characterization of P0 protein in Babesia divergens, a blood parasite of veterinary and zoonotic importance, were carried out in this study for use in developing subunit vaccines against B. divergens infection. Recombinant protein encoding P0 (BdP0) was developed in Escherichia coli, and its antiserum was generated in mice for further molecular characterization. Anti-rBdP0 serum had a specific interaction with the corresponding legitimate B. divergens protein, as confirmed by Western blotting and indirect fluorescent antibody tests. ELISA was used to assess the immunogenicity of BdP0 in a group of 68 bovine field samples, and significant immunological reactivity was found in 19 and 20 positive samples of rBdp0 and B. divergens lysate, respectively. The in vitro growth of B. divergens cultures treated with anti-rBdP0 serum was significantly inhibited (p < 0.05). Furthermore, after 6 h of incubation with 2 mg/ml anti-rBdP0 serum, the ability of pre-incubated free merozoites to invade bovine erythrocytes was reduced by 59.88%. The obtained data suggest the possible use of rBdP0 as diagnostic antigen and may serve as a vaccine candidate against babesiosis caused by B. divergens either in animal or human.

The Gynecologic Health Consequences of Chlamydia trachomatis Infection in Military Servicewomen

2018 ◽  
Vol 36 (06) ◽  
pp. 340-350 ◽  
Author(s):  
Christine Nadeau ◽  
Dennis Fujii ◽  
Jessica Lentscher ◽  
Amanda Haney ◽  
Richard Burney
Keyword(s):  
Chlamydia Trachomatis ◽  
The United States ◽  
Chlamydia Infection ◽  
Tubal Infertility ◽  
Military Health ◽  
Vitro Fertilization ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
The Military

Abstract Chlamydia trachomatis is the most common sexually transmitted bacterial infection in the United States. Within the U.S. military, the age- and race-adjusted chlamydia infection rates among female service members are consistently higher than civilian rates, with a 20% annual acquisition rate among young active-duty women. The sequelae of chlamydia disproportionately impact women in terms of severity and cost. Untreated chlamydia progresses to pelvic inflammatory disease in 40% of cases, and is a leading cause of fallopian tube damage and pelvic adhesive disease resulting in ectopic pregnancy, tubal infertility, and acute and chronic pelvic pain. Tubal infertility is among the leading indications for in vitro fertilization (IVF) nationally and rates among couples undergoing IVF at military treatment centers are double the national average. Collectively, chlamydia infection represents a significant resource burden to the military health care system and, in view of the serious gynecologic health sequelae, a significant threat to the readiness of servicewomen. In this review, we discuss the gynecologic impact of chlamydia infection within the military, the critical gaps for research funding, and opportunities for intervention.

Unsuspected Chlamydia trachomatis infection and in vitro fertilization outcome

1994 ◽  
Vol 171 (5) ◽  
pp. 1208-1214 ◽  
Author(s):  
Steven S. Witkin ◽  
Khalid M. Sultan ◽  
Gregory S. Neal ◽  
Jan Jeremias ◽  
James A. Grifo ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Chlamydia Trachomatis ◽  
Vitro Fertilization ◽  
Chlamydia Trachomatis Infection

scholarly journals An in vitro model of azithromycin-induced persistent Chlamydia trachomatis infection

2017 ◽  
Vol 364 (14) ◽  
Author(s):  
Yaohua Xue ◽  
Heping Zheng ◽  
Zhida Mai ◽  
Xiaolin Qin ◽  
Wentao Chen ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
In Vitro Model ◽  
Chlamydia Trachomatis Infection ◽  
Vitro Model

Antigenic characterization of adult Wuchereria bancrofti filarial nematodes

Parasitology ◽  
1986 ◽  
Vol 93 (3) ◽  
pp. 559-569 ◽  
Author(s):  
T. M. Morgan ◽  
Inge Sutanto ◽  
Purnomo ◽  
Sukartono ◽  
F. Partono ◽  
...  
Keyword(s):  
Culture Medium ◽  
Wuchereria Bancrofti ◽  
Surface Antigens ◽  
Secreted Proteins ◽  
Male And Female ◽  
Antigenic Characterization ◽  
Filarial Nematodes ◽  
Major Surface

SUMMARYAdult Wuchereria bancrofti were recovered from infected Presbytis cristatus monkeys and radio-isotope labelled extrinsically with 125I and in vitro with [35S]methionine. 125I labelling of the surface of adult W. bancrofti permitted a comparison between the major surface antigens of this species and those from the related lymphatic filariae, Brugia malayi and B. pahangi. All species bear a prominent Mr 29 000 surface antigen but among the differences observed were the strongly labelled molecules with Mr 58 000 and 67 000 in W. bancrofti which are extremely faint in the Brugia species. The [35S]methionine label was effectively incorporated into somatic parasite proteins in vitro although it was not possible to identify any secreted proteins in this way. The antigenicity of these products was investigated using a variety of sera from homologous and heterologous infections and the immunoprecipitation patterns highlighted particular differences between somatic proteins of male and female worms. One secreted antigen was detected, however, by virtue of its phosphorylcholine epitopes, in the culture medium of mixed adult worms; medium from male W. bancrofti adults was negative although homogenates of either sex of adult W. bancrofti were strongly positive in the same system.

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